Study on Multiplication and Germination of Protocorm-like Bodies (PLB) in Phalaenopsis sp.
Keywords:BAP, plb, germination, multiplication, NAA, plantlet
Phalaenopsis, one of plant genus in Orchidaceae, is commercially produced as pot plant and cut flower because it has high economical value in world market, including Indonesia. Several micropropagation methods have been developed for Phalaenopsis, but not all of these methods can be used for commercial micropropagation because of differences in survival rate, PLB formation and plantlet regeneration. Therefore, continued studies about the most important stages in Phalaenopsis micropropagation, which are multiplication and germination stages, are needed. Combination of NAA and BAP produce PLB formation and multiplication. Objectives of this study were to determine the effect of NAA and BAP concentrations in PLB multiplication, and germination of PLB from this multiplication results grown in medium without plant growth regulators. Effects of NAA and BAP combinations, which are 0.1 ppm NAA and 1 ppm BAP; 0.1 ppm NAA and 5 ppm BAP; 0.1 ppm NAA and 10 ppm BAP; 0.1 ppm NAA and 20 ppm BAP; 1 ppm NAA and 1 ppm BAP; 1 ppm NAA and 5 ppm BAP; 1 ppm NAA and 10 ppm BAP; 1 ppm NAA and 20 ppm BAP; with control without NAA and BAP, in multiplication medium were observed and compared with the results of PLB germination. PLB multiplication observed on ten weeks of incubation showed that multiplication of Phalaenopsis PLB was optimum in 0.1ppm NAA and 5 ppm BAP medium (20.6 PLBs per PLB and has green color). Statistical analysis one-way ANOVA with post hoc Tukey test (p <0.05 showed that results of PLB multiplication between control medium and all treatment medium was significantly different, except for 0.1 ppm NAA and 5 ppm BAP, 0.1 ppm NAA and 10 ppm BAP, 0.1 ppm NAA and 20 ppm BAP treatments. In 0.1 ppm NAA treatment was resulted more PLB than 1 ppm NAA treatment. Observation of PLB germination was performed after PLB was incubated in germination medium for 42 weeks. This observation showed germination of Phalaenopsis PLB optimally occurred in PLB that has previously cultured in medium multiplication with combination of 1 ppm NAA and 10 ppm BAP (108 plantlets).
Chen, J.T. dan Chang, W.C. Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biologia Plantarium 50 (2): 169-173. 2006
Tokuhara, K. dan Mii, M. Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Reports 13: 7-11. 1993.
Vasil, I.K. Thorpe, T.A.. Plant Cell and Tissue Culture. Dordrecht: Kluwer Academic Publishers. 1994
Pierik, R.L.M.. In Vitro Culture of Higher Plants. Dordrecht: Kluwer Academic Publishers. 1998
George, E.F., Hall, M.A., De Klerk, G-J.. Plant Propagation by Tissue Culture: Volume 1. The Background. 3rd ed. Dordrecht: Springer. 2008
Arditti, J. dan Ernst, R. Micropropagation of Orchids. Canada: John Wiley & Sons. 1993
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