Micropropagation of Phalaenopsis ‘R11 x R10’ Through Somatic Embryogenesis Method


  • Febriya Antensari
  • Totik Sri Mariani School of Life Sciences and Technology, Bandung Institute of Technology,
  • Adhityo Wicaksono


somatic embryo, protocorm like bodies, maltose, sucrose, Phalaenopsis


Phalaenopsis ‘R11 x R10’ is one of hybrid orchids with is a potential commercial orchids as cut flowers and potted plants. Therefore, it is important to increase Phalaenopsis ‘R11 x R10’ production both in quantities and quality. Unfortunately it was difficult to propagate vegetative because Phalaenopsis is monopodial orchid which can not propagate by using keiki. Besides, the availability of good quality orchid seeds is limited due to genetic variability in traditional breeding. Micropropagation method has been providing good quality clones of orchid hybrid in large quantities with minimum genetic variability. In this research two carbohydrate variation (sucrose and maltose) with different concentrations (1%, 3%, and 6%) were tested on PLB proliferation as one of somatic embryogenesis method for improving it. The objective of this study has to evaluate the effect of sucrose and maltose in proliferation stage. The effect of 1%, 3%, and 6% sucrose, 1%, 3%, and 6% maltose, and 3% sucrose as control on proliferation, germination, and plantlet conversion of the PLB. After ten weeks observation, highest proliferation was shown on 3% sucrose as control (71.2 PLBs per PLB). Based on statistical analysis one-way ANOVA with post hoc test tukey test (p<0,05), the result shown significantly different response to sugar concentration and variation. Observation PLB germination was performed after incubating PLB proliferation germination for ten weeks. After eight weeks observation on conversion medium, highest PLB conversion (7 plantlets per ten PLBs) was achieved in PLB with 6% maltose treatment. It is concluded that 6% maltose is the optimum concentration in promoting plantlet conversion.


Author Biography

Totik Sri Mariani, School of Life Sciences and Technology, Bandung Institute of Technology,

School of Life Sciences and Technology

Rank :A


Arditti, J.and R, Ernst. 1993. Micropropagation of Orchids. John Wiley and Sons, Inc. : Canada

Biahoua, A and L. Bonneau. 1999. Control of in vitro somatic embryogenesis of spindle tree (Euonymus europaeus L) by the sugar type and the osmotic potensial of culture medium. Plant Cell Reports 19: 185-190

George, E. F., M.A. Hall and G.J De Klerk. 2008. Plant Propagation by Tissue Culture 3rd Edition Volume I. The Background. Springer : Netherlands

Ishii, Y., T. Takamura, M. Goi and M. Tanaka. 1998. Callus Induction and Somatic Embryogenesis of Phalaenopsis. Plant Cell Reports 17: 446-450

Jheng, F.Y., Y.Y. Do, Y.W. Liauh, J.P. Chung and P.L. Huang. 2006. Enhancement of Growth and Regeneration Efficiency from Embryogenic callus cultures of Oncidium ‘Gower Ramsey’ by Adjusting Carbohydrate Sources. Plant Science 170: 1133-1140

Li, S.H., C.S. Kuoh, Y.H. Chen, H.H. Chen. and W. H. Chen. 2005. Osmotic Sucrose Enhancement of Single-Cell Embryogenesis and transformation Efficiency in Oncidium. Plant Cell, Tissue and Organ Culture 81: 183-192

Park, S.Y., H.N. Murthy, and K.Y. Paek. 2002. Rapid Propagation of Phalaenopsis from Floral Stalk-Derived Leaves. In vitro Cell, Development, Biology-Plant 38: 168-172

Stasolla, C. and E.C. Yeung, E. C. 2003. Recent Advances in Conifer Somatic Embryogenesis: Improving Somatic Embryo Quality. Plant cell. Tissue and Organ Culture 74: 15-35

Trigiano, R.N. and D.J. Gray. 2005. Plant Development and Biotechonolgy. CRC Press LLC : Florida




How to Cite

Antensari, F., Mariani, T. S., & Wicaksono, A. (2014). Micropropagation of Phalaenopsis ‘R11 x R10’ Through Somatic Embryogenesis Method. Asian Journal of Applied Sciences, 2(2). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/1001




Most read articles by the same author(s)