Micropropagation of Phalaenopsis â€˜R11 x R10â€™ Through Somatic Embryogenesis Method
Keywords:somatic embryo, protocorm like bodies, maltose, sucrose, Phalaenopsis
Phalaenopsis â€˜R11 x R10â€™ is one of hybrid orchids with is a potential commercial orchids as cut flowers and potted plants. Therefore, it is important to increase Phalaenopsis â€˜R11 x R10â€™ production both in quantities and quality. Unfortunately it was difficult to propagate vegetative because Phalaenopsis is monopodial orchid which can not propagate by using keiki. Besides, the availability of good quality orchid seeds is limited due to genetic variability in traditional breeding. Micropropagation method has been providing good quality clones of orchid hybrid in large quantities with minimum genetic variability. In this research two carbohydrate variation (sucrose and maltose) with different concentrations (1%, 3%, and 6%) were tested on PLB proliferation as one of somatic embryogenesis method for improving it. The objective of this study has to evaluate the effect of sucrose and maltose in proliferation stage. The effect of 1%, 3%, and 6% sucrose, 1%, 3%, and 6% maltose, and 3% sucrose as control on proliferation, germination, and plantlet conversion of the PLB. After ten weeks observation, highest proliferation was shown on 3% sucrose as control (71.2 PLBs per PLB). Based on statistical analysis one-way ANOVA with post hoc test tukey test (p<0,05), the result shown significantly different response to sugar concentration and variation. Observation PLB germination was performed after incubating PLB proliferation germination for ten weeks. After eight weeks observation on conversion medium, highest PLB conversion (7 plantlets per ten PLBs) was achieved in PLB with 6% maltose treatment. It is concluded that 6% maltose is the optimum concentration in promoting plantlet conversion.
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