Simple Micropropagation by Indirect Somatic Embryogenesis in Sugarcane (Saccharum officinarum L.)

Authors

  • Totik Sri Mariani School of Life Sciences and Technology, Bandung Institute of Technology,
  • Sony Suhandono
  • Dini Ermavitalini
  • Mr. Ihsan
  • Tita Puspita

Keywords:

Sugarcane, 2, 4-D, somatic embryo, plantlet

Abstract

The simple method for producing sugarcane somatic embryos was developed by reducing concentration of 2,4-D. Callus of sugarcane was maintained on MS medium supplemented by 3 ppm of 2,4-D (D3). The callus surface changed into white opaque colour  (white nodule) when the concentration of 2,4-D was decreased to 1.5 ppm (D1.5). Thereafter, the somatic embryos developed from the white nodule. There are various developmental stage of sugarcane somatic embryos on D1.5 medium, namely globular, early scutellar, late scutellar, coleoptilar embryos. When the 2,4-D was omitted from the medium (D0), the coleoptilar somatic embryos germinated , formed shoot and root. The germinated somatic embryos were growing well forming plantlet. This simple method offers a convenient tool for micropropagation of sugarcane.

 

Author Biography

Totik Sri Mariani, School of Life Sciences and Technology, Bandung Institute of Technology,

School of Life Sciences and Technology

Rank :A

References

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Published

2014-04-22

How to Cite

Mariani, T. S., Suhandono, S., Ermavitalini, D., Ihsan, M., & Puspita, T. (2014). Simple Micropropagation by Indirect Somatic Embryogenesis in Sugarcane (Saccharum officinarum L.). Asian Journal of Applied Sciences, 2(2). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/1012

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