Somatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) for Synthetic Seed Production

Authors

  • Totik Sri Mariani School of Life Sciences and Technology, Bandung Institute of Technology,
  • Darjat Sasmitamiharja
  • Devi Mienanti
  • Sjafrul Latif
  • Gale Ginting
  • Hiroshi Miyake

Keywords:

Somatic embryogenesis, oil palm, synthetic seed

Abstract

Somatic embryogenesis of oil palm in liquid medium has been developed since 1999 by Touchet et al. However, synthetic seed production of oil palm has not been performed. Synthetic seed is useful for long time storage and low cost delivery to far away plantation. In the present study, methods from somatic embryogenesis up to synthetic seed production were performed. Friable embryogenic callus was induced, embr yogenic cell suspension was initiated, development and maturation of somatic embryo were conducted. The maturation of somatic embryos were performed in two step. First step was inducing the accumulation of storage protein by arginine and glutamine. Second step was inducing dessication tolerance by Abscisic acid. After the somatic embryos maturing, they were dessicated for 2 hours on sterilized filter paper. Then, dessicated somatic embryo were mixed in 3% alginate solution and dropped one by one to 100 mM CaCl2 solution to form beads of synthetic seeds. The synthetic seeds were then germinated on germination medium containing gibberelic acid. The synthetic seeds germinated after one month on germination media and on sterilized soil.

Author Biography

Totik Sri Mariani, School of Life Sciences and Technology, Bandung Institute of Technology,

School of Life Sciences and Technology

Rank :A

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Published

2014-06-15

How to Cite

Mariani, T. S., Sasmitamiharja, D., Mienanti, D., Latif, S., Ginting, G., & Miyake, H. (2014). Somatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) for Synthetic Seed Production. Asian Journal of Applied Sciences, 2(3). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/1394

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