Somatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) for Synthetic Seed Production


  • Totik Sri Mariani School of Life Sciences and Technology, Bandung Institute of Technology,
  • Darjat Sasmitamiharja
  • Devi Mienanti
  • Sjafrul Latif
  • Gale Ginting
  • Hiroshi Miyake


Somatic embryogenesis, oil palm, synthetic seed


Somatic embryogenesis of oil palm in liquid medium has been developed since 1999 by Touchet et al. However, synthetic seed production of oil palm has not been performed. Synthetic seed is useful for long time storage and low cost delivery to far away plantation. In the present study, methods from somatic embryogenesis up to synthetic seed production were performed. Friable embryogenic callus was induced, embr yogenic cell suspension was initiated, development and maturation of somatic embryo were conducted. The maturation of somatic embryos were performed in two step. First step was inducing the accumulation of storage protein by arginine and glutamine. Second step was inducing dessication tolerance by Abscisic acid. After the somatic embryos maturing, they were dessicated for 2 hours on sterilized filter paper. Then, dessicated somatic embryo were mixed in 3% alginate solution and dropped one by one to 100 mM CaCl2 solution to form beads of synthetic seeds. The synthetic seeds were then germinated on germination medium containing gibberelic acid. The synthetic seeds germinated after one month on germination media and on sterilized soil.

Author Biography

Totik Sri Mariani, School of Life Sciences and Technology, Bandung Institute of Technology,

School of Life Sciences and Technology

Rank :A


Aberlenc-Bertossi, F, Noirot M and Duval Y. 1999. BA enhances the germination of oil palm somatic embryos derived from embryogenic suspension cultures. Plant Cell, Tissue and Organ Culture 56:53-57

Choi, Y.E. and Jeong, J.H. 2002. Dormancy induction of somatic embryo of Siberian Ginseng by high sucrose concentrations enhances the conservation of hydrated artificial seeds abd dehydration resistance. Plant Cell Rep. 20: 1112-1116

Filho, J.C.B and Hattori, K. 1997. Embryogenic callus formation histological studies from Stevia reboudiana (Bert.) Bertoni floret explants. R Bras Fisiol.Veg. 9 (3):185-188

Fitriani, N. 2002. Perkembangan embrio somatik dari kotiledon kacang hijau (Vigna radiata (L) Wilczek). Tesis Pascasarjana ITB. Bandung.

Fujii J A A., Slade D., Aguirre-Rascon J. and Redenbaugh K. 1992. Field Planting of Alfalfa Artificial seeds. In Vitro Cell. Dev. Biol. 28P:73-80

Gray D J, Purohit A. 1991. Somatic embryogenesis and development of synthetic seed technology. Critical Reviews in Plant Sciences 10(1): 33-61.

Gupta P.K., Timmis R., Carlson W., Timmis K, Grob J., Welty E and C. Carpenter. 2000.

Advances in conifer tree improvement through somatic embryogenesis. In Challenges of plant and agriculture sciences to the crisis of biosphere on earth in the 21st century. Watanabe K., & Komamine A (eds.). Landes Bioscience publ. P. 303.

Gupta, P.K., Timmis R., and W. Carlson. 2003. Abstract of Congress on In Vitro Biology. Oregon. P. 14A

Hartmann H.T., Kester D.E., Davies Jr. F.T. and Geneve R.L. 1997. Plant Propagation, Principles and Practices. Sixth edition. Prentice Hall, New Jersey. 130-134

Jayanthi M, Mandal P K. 2001. Plant regeneration through somatic embryogenesis and RAPD analysis pf regenerated plants in Tylophora indica (Burm.f.Merrill.) In Vitro Cell. Dev. Biol. Plant. 37:576-580

Komamine, Atsushi. 2003. My Way with Plant Cell Cultures : Significance of

Experimental Systems in Plant Biology. In Vitro Plant. Vol 39(2) : 63-73. Kreuger, Marc. 1996. Induction of plant somatic embryogenesis in liquid medium. Den Haag.

Mariani T S, Miyake H and Takeoka Y. 1998. Changes in surface structure during direct somatic embryogenesis in rice scutellum observed by scanning electron microscopy. Plant Production Science 1(3): 223-231

Mariani T S., Miyake H., and Takeoka Y. 2000. Improvement of Direct Somatic Embryogenesis in Rice by Selecting the Optimal Developmental Stage of Explant and Applying Desiccation Treatment. Plant Production Science. 3 (2): 114-123

Mariani, T.S., Miyake, H. and Y. Takeoka. 2002. Direct Somatic Embryogenesis in Rice (Oryza sativa L.) : Structural and Developmental patterns. Jurnal Matematika dan Sains. 7(2): 53-56

McKersie, B.D. 1995. Somatic embryogenesis in alfalfa. A model for the development of dry artificial seed technology. IN Seed Development and Germination. J. Kigel and G Galili (eds). Marcel Dekker, NY. Chap. 31: pp. 833-846.

Noerhadi, E. 1988. Embryogenesis somatik sebagai alternatif untuk produksi pengadaan tanaman. Seminar Bioteknologi Tanaman. Jakarta. p.4

Nurwendah, I. 2002. Pengamatan struktur permukaan embrio somatik selama embriogenesis somatik pada bawang putih (Allium sativum L. cv. Lumbu Hijau) dengan scanning electron microscopy (SEM). Skripsi Sarjana ITB. Bandung. Osmotek Ltd. 2002. CD-ROM.

Osuga, K and Komamine , A. 1994. Synchronization of somatic embryogenesis from carrot cells at high frequency as a basis for the mass production of embryos. Plant Cell Tissue Org. Culture. 39. 125-135

Puspitawati, R.P. 1997. Embriogenesis pada kacang hijau (Vigna radiata) varietas wallet. Tesis Sarjana ITB. Bandung.

Ramayanti, O. 2003. Pengaruh metil jasmonat pada embriogenesis somatik Lithospermum erythrorhizon Siebb. Et Zucc. Skripsi. Departemen Biologi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Teknologi Bandung.

Redenbaugh K, Slade D, Viss P and Fujji J A. 1987. Encapsulation of somatic embryos in synthetic seed coats. Hortscience 22(5): 803-809

Tahardi, J.S. Parwono, T.W., Siswanto, Santoso, D and Naatmadja R. (ed.). 1997. Somatic embryogenesis from cell suspension culture of oil palm. Proc. Of the BITG Workshop on oil palm Improvement through Biotechnology. Bogor

Teixeira J B, Sondahl M R & Kirby E G. 1993. Somatic embryogenesis from immature zygotic embryos of oil palm. Plant Cell, Tissue and Organ Culture 34: 227-233

Teixeira J B., Sondahl M R., Nakamura T., & Kirby E G. 1995. Establishment of oil palm cell suspensions and plant regeneration. Plant Cell Tissue and Organ Culture 40:405-411

Tokuhara, K, Mii M. 2001. Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In Vitro Cell. Dev. Biol. Plant 37:457-461.

Tonon G., Berardi G., Rossi C., Bagnaresi U. 2001. Synchronized Somatic Embryo Development in Embryogenic suspensions of Fraxinus angustifolia. In Vitro Plant. Vol 37(4) : 46 2-465

Touchet (de) B, Duval Y and Pannerier C. 1991. Plant regeneration from embryogenic suspension cultures of oil palm (Elaeis guineensis Jacq). Plant Cell Report. 10: 529-532




How to Cite

Mariani, T. S., Sasmitamiharja, D., Mienanti, D., Latif, S., Ginting, G., & Miyake, H. (2014). Somatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) for Synthetic Seed Production. Asian Journal of Applied Sciences, 2(3). Retrieved from




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