In Vitro Anti-melanogenesis and Collagen Biosynthesis Stimulating Activities of Star Grass (<i>Hypoxis aurea </i>Lour.) Extracts

Authors

  • Korawinwich Boonpisuttinant Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathumthani 12130, Thailand
  • Usa Sodamook Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathumthani 12130, Thailand
  • Warintorn Ruksiriwanich Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
  • Supanida Winitchai Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10300, Thailand

Keywords:

Anti-melanogenesis, Collagen biosynthesis, Star Grass, Hypoxis aurea Lour.

Abstract

Star Grass (SG) or Hypoxis aurea Lour. is the Thai traditional plant from genus Hypoxidaceae, which has been used to treat acnes, dark spots and blemish. However, there is no scientific report for bioactivity of SG especially the bioactivity claiming for cosmeceutical uses. This present study was investigated the anti-melanogenesis and collagen biosynthesis stimulating of the SG extracts, in order to evaluate the possible use as a whitening or anti-aging agent for cosmeceuticals. It was found that the phytochemicals detected in these extracts were glycosides (deoxysugars) and tannins. The leave extract from Star grass prepared by the maceration with 95%(v/v) ethanol at ambient temperature (SGLE) demonstrated the highest free radical scavenging activity by DPPH assay, tyrosinase inhibition activity by modified dopachrome method and anti-melanogenesis on B16F10 cells which was comparable to vitamin C (p< 0.05) ; and stimulation of collagen biosynthesis on human skin fibroblasts, which was superior than vitamin C (p< 0.05). This study has suggested that the leaves from Star grass extracted by 95%(v/v) ethanol (SGLE) can be applied as whitening and anti-aging agents for cosmeceuticals.


Author Biography

Warintorn Ruksiriwanich, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand

References

Cheng ZQ, Dan Y, Qing-yun M, Yu-Qing L, Hu JM, Jiang HZ, Wang PZ, Li N, Jun Z, You-xing Z. Two New Phenolic Glycosides from Hypoxis aurea Lour. Bull Korean Chem Soc 2009; 30(10): 2446-2448

Cheng ZQ, Dan Y, Qing-yun M, Yu-qing L, Jun Z, You-xing Z. Chemical Constituents from Hypoxis aurea Lour. Nat Prod Res & Develop 2011; 23: 1002.

Manosroi A, Boonpisuttinant K, Winitchai S, Manosroi J. Free Radical Scavenging and Tyrosinase Inhibition Activity of Oil and Sericin Extracted from Thai Native Silkworms Bombyx mori. Pharm. Biol. 2010; 48(8): 855-860.

García-Molina F, Fenoll LG, Morote JC, García-Ruiz PA, Rodríguez-López JN, García-Cánovas F and Tudela J. Opposite effects of peroxidase in the initial stages of tyrosinase-catalysed melanin biosynthe sis. Inter. J. Biochem. & Cell Biol. 2005; 37: 1179-1196.

Hoogduijn MJ, Cemeli E, Ross K, Anderson D, Thody AJ, Wooda JM. Melanin protects melanocytes and keratinocytes against H2O2-induced DNA strand breaks through its ability to bind Ca2+. Exp. Cell Res. 2004; 294: 60-67.

Briganti S, Camera E, Picardo M. Chemical and Instrumental Approaches to Treat Hyperpigmen tation. Pigment Cell Res 2003; 16(2): 101-110.

Huey-Chun H, Wan-Yu H, Yu-Lin N, Tsong-Min C. Inhibition of melanogenesis and antioxi dant properties of Magnolia grandiflora L. flower extract. BMC Compl. & Alternative Med. 2012; 12(72): 1-9.

Tengamnuay P, Pengrungruangwong K, Pheansri I, Likhitwitayawuid K. Artocarpus lakoocha heartwood extract as novel cosmetic ingredient: evaluation of the in vitro anti tyrosinase and in vivo skin whitening activities. Inter. J. Cosmetic Sci. 2006; 28: 269-276.

Epstein EH. Alpha I (III), Human skin collagen: Release by pepsin digestion and preponderance in foetal life. J. Biol. & Chem. 1974; 249: 3225-31.

Yabe N, Matsui H. Ampelopsis brevipedunculata (Vitaceae) extract stimulates collagen synthesis through superoxide generation in the serum-free cultures of rat dermal fibroblasts and Ito cells. J. Ethnopharmacol. 1997; 56: 67-76.

Manosroi J, Boonpisuttinant K, Manosroi W., Manosroi A. Anti-cancer activities on HeLa cell lines of anti-cancer medicinal recipes selected from Thai/Lanna medicinal plant recipe database MANOSROI II. J. Ethnopharmacol. 2012; 142: 422-431.

Maizura M, Aminah A., Wan WM. Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract. Inter. Food Res. J. 2011; 18: 529-534.

Boonpisuttinant K, Manosroi A, Rahmat D, Monosroi J. Enhancement of in vitro membrane permeation and anti-proliferative on cancer cell lines of aqueous extracts from Thai anti-cancer medicinal plants by entrapping in chitosan and chitosan-TGA nanoparticles. J. Adv. Sci. Lett. 2012;17: 206-216.

Soo-Jin H, Seok-Chun K, Seon-Heui C, Do-Hyung K, Heung-Sik P, Young-Ung C, Daekyung K, Won-Kyo J, You-Jin J. Effect of phlorotannins isolated from Ecklonia cava on melanogenesis and their protective effect against photo-oxidative stress induced by UV-B radiation.Toxicology in Vitro 2009; 23(6): 1123–1130.

Ramires PA, Romito A, Cosentino F, Milella E. The infuence of titania/ hydroxyl apatite composite coatings on in vitro osteoblasts behaviour. Biomaterials 2001; 22: 1467-1474.

Manosroi A, Jantrawut P, Akihisa T, Manosroi W, Manosroi J. In vitro anti-aging activities of Terminalia chebula gall extract. Pharmaceutical Biol 2010; 1-13.

Oladele KA, Oluwatooyin OF, Yemisi AA. Influence of Processing Techniques on the Nutrients and Antinutrients of Tigernut (Cyperus esculentus L.). World J. Dairy & Food Sci. 2009; 4(2): 88-93.

Zulkifli KS, Abdullah N, Abdullah A, Aziman N, Kamarudin WSSW. Bioactive Phenolic Compounds and Antioxidant Activity of Selected Fruit Peels. International Conference on Environment. Chem & Biol 2012;49(14): 66-70.

Gomez-Cordove C, Bartolome B, Vieira W, Virador VM. Effects of wine phenolics and sorghum tanninson tyrosinase activity and growth of melanoma cells. J. agri. & food chem 2001;49(3): 1620-1624.

Sergent T, Piront N, Meurice J, Toussaint O, Schneider YJ. Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium. Chemico-Biological Interact. 2010; 188(3): 659-667.

Fawole OA, Makunga NP, Opara UL. Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract. BMC Compl. & Alternative Med. 2012; 12(200): 2-11.

Kozina LS, Borzova IV, Arutiunov VA, Ryzhak GA. Role of Oxidative Stress in Skin Aging. Advances in Gerontol. 2013; 3(1): 18–22.

Pujimulyani D, Raharjo S, Marsono Y, Santoso U. The effect of blanching on antioxidant activity and glycosides of white saffron (Curcuma mangga Val.). Inter. Food Res. J. 2012; 19(2): 617-621.

Oszmianski J, Wojdylo A, Lamer-Zarawska E, Swiader K. Antioxidant tannins from Rosaceae plant roots. Food Chem. 2007; 579-583.

Chang TS. Natural Melanogenesis Inhibitors Acting Through the Down-Regulation of Tyrosinase Activity. Materials 2012; 5: 1661-1685.

Jenq-Sheng C, Ke Liang BC , Deng-Fwu H, Zwe-Ling K. In Vitro Cytotoxicitiy of Silica Nanoparticles at High Concentrations Strongly Depends on the Metabolic Activity Type of the Cell Line Article. Environ. Sci. Technol. Lett. 2007; 41(6): 2064-2068.

Calabrese V, Cornelius C, Cuzzocrea S, Iavicoli I, Rizzarelli E.Hormesis, cellular stress response and vitagenes as critical determinants in aging and longevity. Molecular Aspects of Medicine. 2011; 32(4-6): 279-304.

Kawabata T, Cui MY, Hasegawa T, Takano F, Ohta T. Anti-inflammatory and anti-melanogenic steroidal saponin glycosides from Fenugreek (Trigonella foenumgraecum L.) seeds. Planta Med. 2011; 77(7): 705-10.

Zhong-Quan C, Dan Y, Yu-Qing L, Jiang-Miao H, He-Zhong J, Peng-Cheng W, Ning L, Jun Z, You-Xing Z. Two New Phenolic Glycosides from Hypoxis aurea Lour. Bull. Korean Chem. Soc. 2009; 30(10): 2446-2448.

Phetdee K., Rattanananee K, Teaktong T, Viyoch J. Tamarind seed coat extract reduces melanin production via tyrosinase melanocytes. J. Biol. Sci. 2012; 12(4): 239-245.

Agyare C, Lechtenberg M, Deters A, Petereit F, Hensel A. Ellagitannins from Phyllanthus muellerianus (Kuntze) Exell.: Geraniin and furosin stimulate cellular activity, differentiation and collagen synthesis of human skin keratinocytes and dermal fibroblasts. Phytomed. 2011; 18: 617–624.

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Published

2014-08-14

How to Cite

Boonpisuttinant, K., Sodamook, U., Ruksiriwanich, W., & Winitchai, S. (2014). In Vitro Anti-melanogenesis and Collagen Biosynthesis Stimulating Activities of Star Grass (<i>Hypoxis aurea </i>Lour.) Extracts. Asian Journal of Applied Sciences, 2(4). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/1531

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