Identification of Bacteria and Fungi Contamination of Some Classrooms at Srinakharinwirot University, Ongkharak Campus

Authors

  • Pattakorn Buppan Department of Health Promotion, Faculty of Physical Therapy, Srinakharinwirot University, 63 Moo 7 Rangsit-Nakhon Nayok Highway, Tambon Ongkharak, Amphur Ongkharak, Nakhonnayok 26120
  • Apisara Sommatas
  • Pareena Nakprasom
  • Natawan Noicharoen
  • Dollava Sangsuvan

DOI:

https://doi.org/10.24203/ajas.v7i5.5983

Keywords:

microorganism in the air, indoor air quality, classroom, contamination

Abstract

The air quality of air inside the classroom is an important factor effect to health of people. The objective of the study was to estimation the air quality and identification of bacteria and fungi contamination in the air inside the classroom at Srinakharinwirot University Ongkharak Campus. The open plate technique was used to collect samples in the morning at 8.30 a.m. (before students enter to the classroom) and in the afternoon at 4.30 p.m. (after students left the classroom) between November 2018 to April 2019. The result showed that the average amounts of bacteria and fungi were 67.60 to 352.50 and 69.60 to 370.80 CFU/dm2/h, respectively. The evaluations of the index of microbial air contamination (IMA) were fair to very poor. However, the concentrations of airborne bacteria and fungi were not higher than the proposed air quality index. Therefore, the air quality of classrooms had a good hygienic standard. The most isolated bacteria were Enterobacter spp., Escherichia coli, Staphylococcus spp., Bacillus spp., and Pseudomonas spp. and fungi were found to include Cladosporium spp., Aspergillus spp., Penicillium citrinum and Neurospora crassa. So, the air quality of air inside classroom control management is appropriate to be used to reduce bacteria and fungi contamination in the classroom.

 

Author Biography

Pattakorn Buppan, Department of Health Promotion, Faculty of Physical Therapy, Srinakharinwirot University, 63 Moo 7 Rangsit-Nakhon Nayok Highway, Tambon Ongkharak, Amphur Ongkharak, Nakhonnayok 26120

Department of Health Promotion, Faculty of Physical Therapy, Srinakharinwirot University

References

Hayleeyesus SF, Manaye AM. Microbiological quality of indoor air in University Libraries. Asian Pacific J Trop Biomed. 2014; 4: 312-317.

Liu MH, Tung TH, Chung FF, Chuang LC, Wan GH. High total volatile organic compounds pollution in a hospital dental department. Environ Monit Assess. 2017; 189: 571-578.

Wemedo SA, Ede PN, Chuku A. Interaction Between Building Design and Indoor Airborne Microbial Load in Nigeria. Asian J Biol Sci. 2012; 5: 183-191.

Gilbert Y, Veillette M, Duchaine C. Airborne bacteria and antibiotic resistance genes in hospital rooms. Aerobiologia. 2010; 26: 185-194.

Huttunen R, Åttman E, Aittoniemi J, Outinen T, Syrjänen J, Kärki T, Lyytikäinen O. Nosocomial bloodstream infections in a Finnish tertiary care hospital: a retrospective cohort study of 2175 episodes during the years 1999–2001 and 2005–2010. Infect Dis (Lond). 2015; 47: 20-26.

Alangaden JG. Nosocomial fungal infections: epidemiology, infection control, and prevention. Infect Dis Clin North Am. 2011; 25: 201-225.

Patterson KC, Strek ME. Diagnosis and treatment of pulmonary aspergillosis syndromes. Chest. 2014; 146: 1358-1368.

Vonberg RP, Gastmeier P. Nosocomial aspergillosis in outbreak settings. J Hosp Infect. 2006; 63: 246-54.

Perdelli F, Sartini M, Spangnolo AM, Dallera M, Lomardi R, Cristina ML. A problem of hospital hygiene: the presence of Aspergilli in hospital wards with different air-conditioning features. Am J Infect Control. 2006; 34: 264-268.

Kavita N, Jyoti G. Microbial air contamination in a school. Int J Curr Microbiol App Sci 2013; 2: 404-410.

Pasquarella C, Pitzurrat O, Savino A. The index of microbial air contamination. J Hosp Infect. 2000; 46: 241-256.

Leyral G, Joffin JN. 1998. Technical Microbiology: 2, Technical Documentation. 2nd Edition, CRDP d’Aquitaine, Bordeaux, 304 p.

Rajash B, Rattan LI. Essential of Medical Microbiology. 4th Edition, Jayppe Brothers Medical Publishers, New Delhi, 2008; 415-451.

Department of Occupational Safety and Health, Ministry of Human Resources, Malaysia. Industry Code of Practice on Indoor Air Quality 2010.

Stryjakowska-Sekulska M, Piotraszewska-Pajak A, Szyszka A, Nowicki M, Filipiak M.Microbiological quality of indoor air in university rooms. Polish J Enviro Stud. 2007; 16: 623-632.

Jakkapong N, Sirilak C, Waralee B. Microbial air contamination in laboratory rooms, faculty of science, Payap University. KKU Sci J. 2014; 42: 341-349.

Siriporn S, Ganjana N. Ambient microbial contamination in different hospital scales. KKU Res J. 2012; 12: 92-101.

Krisaneeya S. Airborne bacteria and fungi in the hospital and the sampling method comparison. Thai J Health Promot Environ. 2005; 29: 113-224.

Downloads

Published

2019-10-18

How to Cite

Buppan, P., Sommatas, A., Nakprasom, P., Noicharoen, N., & Sangsuvan, D. (2019). Identification of Bacteria and Fungi Contamination of Some Classrooms at Srinakharinwirot University, Ongkharak Campus. Asian Journal of Applied Sciences, 7(5). https://doi.org/10.24203/ajas.v7i5.5983