Effect of Gonadotrophic Hormones and Insulin-like Growth Factor I (IGF-i) on Maturation of Mammalian Oocytes in vitro

Abd El Wahab El Ghareeb, Amr El Ahwany, Mohamed Khamis, Tawfik Al Aref

Abstract


Mammalian oocyte in vitro maturation (IVM) is a fundamental technique in human-assisted reproduction clinic and reproductive biology research, which can provide high quality oocytes for gamete and embryo engineering. However, IVM remains a challenge in mammalian species, and has only been achieved with limited degrees of success. A number of hormones and growth factors have been reported to affect the in vitro maturationof oocytes. In the present study, we have investigated the effects of gonadotrophic hormones and insulin-like growth factor 1(IGF-I) on the in vitro maturation of rabbit oocytes.

Our study was carried out to investigate the role of adding follicle-stimulating hormone (FSH), luteinizing hormone (LH) or the combination of both hormones with Insulin-like growth factor 1 (IGF-I) on the IVM of rabbit oocytes, as rabbit is considered as an important model system for human reproduction. Unstimulated ovaries of female rabbits were encountered. Oocytes were collected using slicing technique. The cumulus oocyte complexes (COCs) containing immature oocytes at germinal vesicle (G.V) stage were counted, washed, and then cultured in different concentrations of FSH, LH, combination of both FSH and LH with Insulin-like growth factor 1 (IGF-I) .The oocyte maturation was examined and our study revealed that the addition of certain concentrations of hormone combinations (FSH + LH) with Insulin-like growth factor 1 (IGF-I) could significantly improve the IVM and increase the percentage of oocytes reaching MII phase.

 


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