Oocyte Activation By Calcium Ionophore A23187 in Intracytoplasmic Sperm Injection
Keywords:Calcium ionophore A23187, fertilization rate, embryos quality, oocyte activation
The present study evaluated the effect of calcium ionophore A23187 on oocytes in intracytoplasmic sperm injection (ICSI) cycles using sperm from different sources. The 80 cycles evaluated were divided into four subgroups according to sperm quality and origin (normospermia- oligoathenoteratospermia â€“ epidermal â€“testicular), subgroups were further split into experimental groups, depending on whether or not treated with calcium ionophore. For each experimental subgroup, ICSI outcomes were compared between groups. In conclusion we found that treatment the oocyts with calcium ionophore increase the fertilization rate in oligoathenoteratospermia, epididymal (PESA) & testicular (TESE) patients without any effect on the embryos quality.
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