GUS Gene Expression in Somatic Embryo of Oil Palm (<i>Elaeis guineensis</i> Jacq.)

Authors

  • T. S. Mariani School of Life Sciences and Technology, Bandung Institute of Technology, Ganesha 10, Bandung 40132, Indonesia
  • D. Ermavitalini
  • T. Mitsutaka
  • T. F. Chia
  • H. Miyake

Abstract

Oil palm is an oil producing plant, which produce 5 to 7 tons of oil/ acre/ year. In this study, we carried out transformation of oil palm by GUS gene. The expression of GUS gene was obtained in 25 minutes soaking and 5 minutes vacuum infiltration method. The colour of GUS gene expression is blue.

Author Biography

T. S. Mariani, School of Life Sciences and Technology, Bandung Institute of Technology, Ganesha 10, Bandung 40132, Indonesia

A

References

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o Mariani, T.S., Sjafrullatif, Wardjo, N.G., Miyake, H. 2014a. Initiation and proliferation of globular somatic embryos of oil palm (Elais guineensis Jacq.) observed by inverted and transmission electron microscopy. International Journal of Basic and Applied Sciences. Vol 14 (01):1-7

o Mariani, T.S., Sasmitamihardja, D.,Mienanti,D., Sjafrullatif, Ginting, G., Miyake, H. 2014b. Somatic embryogenesis of oil palm (Elaeis guineensis Jacq.) for synthetic seed production. Asian Journal of Applied Sciences. Vol 02(03): 358-367

o Muad, M.R. (2000). Genetic transformation of oil palm by chitinase gene mediated by Agrobacterium tumefaciens. School of Bioscience and Biotechnology, Faculty of Science and Technology, UKM

o Parveez, G.K.A., Chowdhury, M.K.U, and Saleh, N.M. (1998). Biological parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) embryogenic calli via microprojectile bombardment, An International Journal Industrial Crops and Products. 8:17-27

o Steeves, T.A. and Sussex, I.M. 1994. Patterns in Plant Development. Second Edition. Cambridge University Press.

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Published

2015-10-26

How to Cite

Mariani, T. S., Ermavitalini, D., Mitsutaka, T., Chia, T. F., & Miyake, H. (2015). GUS Gene Expression in Somatic Embryo of Oil Palm (<i>Elaeis guineensis</i> Jacq.). Asian Journal of Applied Sciences, 3(5). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/3109