TY - JOUR AU - Mariani, Totik Sri AU - Purnaning, Arry Setya AU - Latif, Dan Sjafrul PY - 2014/10/15 Y2 - 2024/03/29 TI - Effect of Glutamine Addition in Maturation Stage on the Germination and Plantlet Conversion of Oil Palm (Elaeis Guineensis Jacq.) Somatic Embryo JF - Asian Journal of Applied Sciences JA - AJAS VL - 2 IS - 5 SE - Articles DO - UR - https://www.ajouronline.com/index.php/AJAS/article/view/1696 SP - AB - <p>The enhancement of oil palm productivity due to increasing of world demand should be supported by stocks of good quality seedling. Besides from the seed, recently the oil palm seedling has been produced through micropropagation of oil palm. The method of oil palm somatic embryogenesis could provide seedling in large quantity with minimum genetic variation. However, there is still a problem in embrio germination and plantlet conversion due to immature somatic embryo. In this study, we performed evaluation of the effect of glutamine amino acid in maturation stage on germination and plantlet conversion of somatic embryo. In addition, we also conducted histological evaluation of germinated somatic embryo by using light microscopy. The observation of germination and plantlet conversion was performed after incubating on the medium supplemented with glutamine for 3 weeks. The germination medium was without glutamine. The result showed that the highest germination percentage (82.5%) in germination medium for 10 weeks. This result after incubated on  the medium supplemented with 10 mM glutamine for 3 weeks. Whereas the conversion into plantlet was achieved on the medium supplemented with 20 mM glutamine.</p> ER -