TY - JOUR AU - Mohammed, S. M. H. AU - Khalil, E. A. G. AU - Idris, F. M. PY - 2014/10/31 Y2 - 2024/03/28 TI - Toll-Like Receptor-2 Gene [TLR2] Polymorphism and Susceptibility to Mycobacteria Tuberculosis Infections in Sudanese Patients JF - Asian Journal of Applied Sciences JA - AJAS VL - 2 IS - 5 SE - Articles DO - UR - https://www.ajouronline.com/index.php/AJAS/article/view/1571 SP - AB - <p>Tuberculosis [TB] is a major public health threat and a leading cause of death worldwide. Innate immunity plays an important role in the host defense against <em>M. tuberculosis</em> through recognition Toll-Like Receptors [TLRs]. Polymorphisms in <em>TLR2 [Arg753Gln]</em> gene have been reported to be associated with increased susceptibility to TB.<strong> </strong>This study aimed to determine possible roles for <em>TLR2 [Arg753Gln]</em> gene polymorphism in susceptibility to <em>M. tuberculosis</em> infections in a cohort of Sudanese individuals. Following informed consent, 141 individuals with smear positive tuberculosis and latent TB infections [mean age 19±38.3 years; male: female ratio  2:1]  and 197   apparently healthy individuals [mean age 30±14; male female ration of 1:5] were enrolled. DNA was extracted from EDTA-blood using the guanidine chloride method. PCR was carried out using <em>TLR2 gene</em> specific primers, Forward: [5'CATTCCCCAGCGCTTCTGCAAGCTCC-3'] and Reverse: [5'GGAACCTAGGACTTTATCGCAGCTC-3')]. Genotyping was done by RFLP using <em>MSP1</em>restriction enzyme. The <em>TLR2 Arg753Gln</em> mutant allele was not detected in any of the study groups.<strong> </strong><em>TLR2 Arg753Gln</em> gene  polymorphisms plays little or no role in host susceptibility to<em> Mycobacterium tuberculosis</em> infections  in Sudanese population.</p><p> </p> ER -