Genetic Relatedness among Iraqi, Jordanian and Iranian TYLCV Based on the Identity Analysis
Keywords:
Begomovirus, Geminivirus, PCR amplification products, Basrah, electorphoresis profileAbstract
To look for genetic relatedness among a new iraqi Tomato Yellow Leaf Curl Virus (TYLCV) and the Jordanian and Iranian ones, identity coefficient was calculated. Amplification Chain Reaction (PCR) technique was used for this purpose from degenerated primers of the begomoviruses coat protein gene. A identity from 94 to 95% was calculated, indicating that the Iraqi isolate is effectively a TYLCV. From the viewpoint of the phyletic relationships, the identity between Iraqi and Iranian TYLCV isolates was 95%, as against 85% for those from Iraq and Jordan. However, it was showed that DNA from Iraqi is not of recombinant origin, implying that these three isolates separately evolve.
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