Oxidative Stress and Antioxidant Vitamins Status of Diabetic Subjects Supplemented with Antioxidant Rich Supplement (<em>Alphabetic</em>) Attending Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria
Keywords:Antioxidant, Diabetic, Malondialdehyde, hyperglycemia, Alphabetic
Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion / insulin action or both which could leads to increase level of oxidative stress and decrease in antioxidant defense system and may play role in the development of diabetic complications. The present study examines the effects of antioxidant rich supplements (Alphabetic) on antioxidant status of one hundred (100) diabetic subjects attending Usmanu Danfodiyo University Teaching Hospital (UDUTH) for at least one year and the results were compared with those of fifty (50) diabetes mellitus non-supplemented and fifty (50) apparently healthy non-diabetic subjects. Standard analytical procedures were used for all the parameters measured including the effects of supplementation on Fasting Blood Glucose, oxidative stress marker (MDA), Reduced gluthathione and antioxidant vitamins (C, E and Î²-carotene) parameters were evaluated. The results revealed that the supplementation significantly (PË‚0.05) reduce Serum glucose and Malondialdehyde and also significantly (PË‚0.05) increases Vitamin C, Vitamin E and Reduced glutathione compared with the non-supplement group. The findings suggests that supplementation with Alphabetic might reduce the risk of oxidative stress associated with diabetes mellitus leading to diabetic complications and might be beneficial in the routine treatment of diabetes mellitus.
Saidu, Y., Nwachukwu, F.C., Bilbis, L.S., Faruk, U.Z. and Abbas, A.Y. (2010). Hypoglycaemic and Hypolipidemic Effects of Root Extracts of Albizzia chevalieri in Alloxan-induced Diabetic Rats. Nig J of Basic and Applied Sci. 18(1): 72-78.
Ladan, M.J., Umar R.A., Hassan S.W., Shehu B. (2007). Glycemic Status and Lipid Profile of Diabetes in Sokoto, Nigeria. Asian J of Biochem. 2: 183- 187
ADA (American Diabetes Association). (2012). â€œDiagnosis and classification of diabetes mellitus,â€ Diabetes Care, 35(1). Pp. 64â€“71. Antioxidants: A review. J. of Biochem and Mol. Tox., 17(1): 24.
Cade, W. T. (2008)â€œDiabetes-related microvascular and macrovascular diseases in the physical therapy setting,â€ Phy. Ther., 88(11): 1322â€“1335.
Whiting, D.R., Guariguata, L., Wei,l C. and Shaw, J. (2011). IDF Diabetes Atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Res Clin Pract. 94:311â€“321.
Shaw, J.E. , Sicree, R.A., Zimmet, P.Z. (2010). Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res. Clin. Pract. 87: 4-14.
WHO (World Health Organization) Report (2011). Prevalence of Diabetes Mellitus. Retrieved on 2012-11-10-11.
Wild, S., Roglic, G., Green, A., Sicree, R. and King, H. (2004). Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Dia. Car., 27: 1047-1053.
Leduc, C., Coonishish, J., Haddad, P. and Cuerrier, A. (2006). Plants used by the Cree Nation of Eeyou Istchee (Quebec, Canada) for the treatment of diabetes: a novel approach in quantitative ethnobotany. J Ethnopharmacol. 105: 55-63.
Amos, A.F., McCarty, D.J. and Zimmet, P. (1997). The Rising Global Burden of Diabetes and its complication: Estimates and Projections to the year 2010. Dia. Med. 14:81-85.
Chase, H.P., Jackson, W.E., Hoops, S.L., Cockerham, R.S., Archer, P.G. and Oâ€™Brien, D. (1989). Glucose control and the renal and retinal complications of insulin-dependent diabetes. JAMA. 261:1155â€“1160.
Bilbis, L. S. (2008). Ê»Friends or FoesÊ¼ Reactive oxygen species in health and diseases. Usmanu Danfodiyo University Central University Serminar Series, Augues 28th 2008. Pp. 14
Chong, Z.Z., Li, F. and Maiese, K. (2005). Oxidative stress in the brain: Novel cellular targets that govern survival during neurodegenerative disease. Prog Neurobiol., 75: 207-246.
Ha, H. and Lee, H.B. (2000). Reactive oxygen species as glucose signaling molecules in mesangial cells cultured under high glucose. Kidney Int., 77: 19-25.
Maritim, A.C., Sanders, R.A. and Watkins, J.B. (2003). Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxico.l 17: 24-38
Rains, J. L., & Jain, S. K. (2011). Oxidative stress, insulin signaling, and diabetes. Free Radic Biol Med. 50(5): 567-575.
Daniel, W. W. W. (1999). Biostatistics: a foundation for analysis in the health science. 7th Ed. New York, NY: Wiley. 180- 185, 268- 270.
Trinder, P. (1969). Determination of blood glucose in blood using glucose oxidase with an alternative oxygen acceptor, Annals of Clin Biochem, 6:24-25.
Pett, L. B., (1940). Fat soluble vitamins. Ann. Rev. of Biochem. 14: 525-560.
Baker, H. and Frank, O. (1986). Determination of serum tocopherol. In: clinical biochemistry, 6th edition. Eimememenn medical books, London, Pp; 902-903.
Hashim S. A. and Schuttringer G. R. (1966). Rapid determination of tocopherol inmacro- and microquantities of plasma. Results obtained in various nutrition and metabolic studies. Am. J. Clin. Nutri; 19(2):137- 14.
Patterson, J.W., Lazarow, A. (1959). Sulfhydryl protection against dehydroascorbic acid diabetes. J. Biol Chem., 186: l41-144.
Shah, J. K. and Walker, A. M. (1989). Quantitative Determination of Malondialdehyde, Biochem. Biopy. Acta. 15 (11): 207-211.
Asayama, K., Uchida, N., Nwakele, T., Hayashibe, H. and Dobashi, K., (1993). Antioxidant in serum of children with type l DM. Free rad. Bio med. 15(6):597-602
Zingg, J. M., Ricciarelli, R. and Azzi, A. (2000). Scavenger receptors and modified lipoproteins. Fatal attraction. IUBMB Life. 49:397-403.
Paolisso, G. and Giugliano, D. (1996). Oxidative stress and insulin action: is there a relationship? Diabetologia. 39:357-36Diseases and Disorders Vol
Kalaivanam, K.N. Mala, D., Sara, R. (2006). Lipid peroxidation in type ll diabetes mellitus. I J of dia in develop coun. 26:30-32
Fridivich, I. (1995). Superoxide radical and superoxide dismutase. Ann rev biochem. 64:97-112.
George, O. (2009). Antioxidants properties of Vitamins. J of American Fam phy. 60: 895-904
Mates, J.M. and Sanchez-Jimenez, F.M. (1999). Antioxidant enzymes and their implications in pathophysiologic processes. Front Biosci. 15: 339-345
Satyanarayana, U., and Chakrapani, U. (2006). Biochemistry. Books and Allied (P) Ltd. Third edition Pp: 409-412.
Sarita, A. Shinde, Anita, D. Deshmukh, Adinath, N. Suryakar, Umesh, K. More, Mona, A. Tilak. (2014). The levels of oxidative stress and antioxidants in diabetes mellitus before and after diabetic treatment with or without antioxidants. Indian J of Basic and App Med Res. 3(2): 455-460.
Britton, K., Baynes, J.W., Peter, U. (1995). Role of oxidative stress in development of complication of diabetes. J of Dia. 40: 305-320
West, I. C. (2000). Radicals and oxidative stress in diabetes. Diabet Med. 17:171â€“ 180.
Aliyu, M., Lawal, M., Mojiminiyi, F., Saidu, Y. and Bilbis, L.S. (2005). Serum antioxidant vitamin levels in in Non-insulin dependant diabetes mellitus subject in Sokoto, Nigeria. J Nig. Soc. For exper. biol.17(2): 107-114
Moorodian, A.D. and Morley, B. (1987). Narrative review a rational approch to stating insulin therapy. Ann Inter med. 145(2): 125-134.
Rasilainens, K. Kuther, S.R. and Peter U.K. (2002). Type ll DM. J dia. 82:201-212
Polidori, M. C. (2005). Plasma level of lipophilic antioxidants in every old patients with type ll diabetes. Dia met res rev. 16:15-19
Broekmans,W.M.R., Klopping-Ketelaars, C.R.W.C and Schuurman (2000). Fruits and vegetable increase plasma carotenoids and Vitamins reduce homocysteine in human. J of Nutri 130(6): 1578-1583.
Pryor W.A. (1996). Vitamin E and Carotenoid Abstracts. 1994 Studies of Lipid-Soluble Antioxidants. Vim. E Resrch and Inform. Serv.publishers.
Fuller, C. J. Chandalia, M. and Garg, A. (1996). Alpha tocopheryl acetate supplementation at pharmacological doses decrease low-density lipoprotein oxidative susceptibility but not protein glycation in patients with diabetes mellitus. A J clinical nitri., 63: 753-759
Chung, A. M., Yu, J.J. and Liu, D.Z. (1998). Alpha tocopherol reduces lipid peroxidation stress of erythrocyte membrane in type ll diabetes mellitus patients with retinopathy. Dia., 40: 380-385
Terry, C. (2005). Diabetes Mullitus. J of clin nutri. 82: 3685-3698
Wali,U., M.U Jogana, A.L Zarummai and Y. Saidu. (2011). Antioxidant Vitamins and Trace Elements Status of Diabetics in Sokoto, Nigeria. Nig J of Basic and App Sci. 19(1):130- 134.
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