Antimicrobial, Antioxidant Activities <i>in vitro </i>and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant
Keywords:Antimicrobial, antioxidant, free radicals, Combretum racemosum, polyphenols
AbstractThis study assessed polyphenolic contents, antioxidant and antimicrobial activities of the solvent fractions obtained from Combretum racemosum leaf extract. Liquid-liquid extraction method was employed to separate the crude aqueous methanolic leaf extract of C. racemosum into n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (n-BuOH) fractions. Antibacterial activities were determined using agar- well diffusion and agar dilution methods. In vitro free radical scavenging activity of the fractions was measured by 2, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picryl dihydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) and nitric oxide (NO) assays. MIC of the EtOAc fraction ranged from 0.63 mg/ml to 1.25 mg/ml while that of n-BuOH and DCM fractions ranged from 0.31mg/ml to 1.25 mg/ml and 0.31 mg/ml to 0.63 mg/ml respectively. The n-BuOH fraction contains highest phenolic compounds (phenol and flavonoids), followed by EtOAc, crude, hexane and DCM. Total antioxidant capacity of the fractions follows the same order as the antioxidant. NO radical scavenging activities of all the fractions showed stronger activity than that of the reference ascorbic acid (AA). The greatest antiradical activity was found in butanol fraction; IC50 for DPPH, NO and FRAP are 38.39Â±7.20Î¼g/ml; 0.11Â±0.03 Î¼g/ml and 180.5203Â±0.86mg/ml respectively. Our findings indicate the potentials of C. racemosum leaf extracts as source of antioxidants and antimicrobials of natural origin
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