Release Activity of Encapsulated <i>Lactobacillus plantarum </i>NBRC 3070 in Optimum Alginate - <i>Aloe vera</i> Matrices during Simulated Gastric Fluid (SGF) and Simulated Intestinal Fluid (SIF) Exposure
Keywords:Probiotic, Microbial Biotechnology
Probiotic encapsulation approach has the potential to protect microorganisms and to deliver them into the gut. Because of the promising preclinical and clinical results, probiotics have been incorporated into a range of products. However, there are still many challenges to overcome with respect to the encapsulation process and the conditions prevailing in the gut. Thus in this study, the release activity of encapsulated L. plantarum NBRC 3070 and Aloe vera gel within alginate coated chitosan matrices during simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) exposure were investigated. There were four groups of beads prepared in this study: 1) Encapsulated probiotic and Aloe vera within alginate beads (chitosan coated), 2) Encapsulated probiotic within alginate beads (chitosan coated), 3) Encapsulated probiotic and Aloe vera within alginate beads (uncoated) and 4) Encapsulated probiotic alone within alginate beads (uncoated). Encapsulation process was carried out using extrusion method. The optimized composition of alginate matrix (1.34% w/v) and Aloe vera gel (1.99% w/v) were used.Â In order to investigate their release activity, all beads were exposed in Simulated Gastric (SGF) at pH 2.5 and Simulated Intestinal Fluids (SIF) at pH 6.5 for 120 min and 270 min, respectively. Based on the findings, alginate-Aloe vera beads with chitosan coated was able to protect L. plantarum NBRC 3070 during SGF exposure with only 1 log10 cfu/mL reduction. The presence of Aloe vera gel in the beads improved the survivability of the cells. Encapsulated cells were observed successfully slow released of cells from the beads after exposure in SIF. Scan Electron Microscope (SEM) result had shown that cross link activity of the optimum alginate-Aloe vera with chitosan coating resulted in better survival of cells after simulated gastro and able to deliver sufficient probiotic dose to intestinal region. The combinations were able to improve encapsulated cells survivability during low acidic environment passage and release activity into the intestinal target region.Â Â
Goldin, B. R., â€œProbiotics and Health Claims: From History to
Future; Probiotics and Health Claimsâ€, First Edition, Blackwell
Publishing Ltd, United Kingdom, 2011.
Sanders, M. E., "Probiotics: Definition, Sources, Selection, and
Uses", Clinical Infectious Diseases, 46 (Supplement 2), pp.
Kotzampassi, K., E. J. Giamarellos-Bourboulis, "Probiotics for
infectious diseases: More drugs, less dietary supplementation",
International Journal of Antimicrobial Agents, vol. 40, no 4, pp.
Chen, S., Q. Zhao, "Development of a novel probiotic delivery
system based on microencapsulation with protectantsâ€, Applied
Microbiology and Biotechnology, vol. 93, no. 4, pp. 1447-1457,
Vernazza, C. L., Rabiu, B. A., Gibson, G. R., â€œHuman colonic
microbiology and the role of dietary intervention: Introduction
to prebioticsâ€, Prebiotics: Development and application,
Chichester: John Wiley & Sons Press, pp. 1â€“2, 2006.
N. P. Shah, A. G. d. Cruz and J. d. A. F. Faria, â€œProbiotic and
Prebiotic Foods: Technology, Stability and Benefits to Human
Healthâ€, New York, Nova Science Publisher, Inc. pp. 25-39,
Nazirah, M. Y. H., S. N. Syahirah, â€œScreening of Aloe vera
medium with different carbon and nitrogen sources for
Lactobacillus acidophilus cultivation using fractional factorial
design (FFD)â€, Proceedings - 2013 IEEE 3rd International
Conference on System Engineering and Technology, ICSET,
Moore, S and Kailasapathy, K., â€œMicroencapsulation: Science
and technologies for probiotics and prebiotics. In: Probiotic and
prebiotic Foods: Technology, Stability and Benefits to the
Human Healthâ€, Nova Science Publishers, NY. ISBN. 978-1-
Syahirah, S. N., Y. Anida, â€œOptimization of Aloe vera and
alginate as encapsulating matrices for Lactobacillus acidophilus
using FCCD-RSM approachâ€, Proceedings - 2013 IEEE 3rd
International Conference on System Engineering and
Technology, ICSET. 2013
Krasaekoopt, W., B. Bhandari, â€œEvaluation of encapsulation
techniques of probiotics for yoghurtâ€, International Dairy
Journal, vol. 13, no.1, pp. 3-13, 2003.
Annan, N. T., A. D. Borza, â€œEncapsulation in alginate-coated
gelatin microspheres improves survival of the probiotic
Bifidobacterium adolescentis 15703T during exposure to
simulated gastro-intestinal conditionsâ€, Food Research
International, vol. 41, no.2, pp.184-193, 2008.
Ling-Xia Pan, Xiu-Juan Fang, Zhen Yu, Yang Xin, Xiao-Ying Liu,
Lu-E Shi, Zhen-Xing Tan., â€œEncapsulation in alginateâ€“skim milk
microspheres improves viability of Lactobacillus bulgaricus in
stimulated gastrointestinal conditionsâ€, Journal International
Journal of Food Sciences and Nutrition, vol.64, no. 3, 2013.
GullÃ³n B1, GullÃ³n P, Tavaria F, Alonso JL, Pintado M., â€œIn vitro
assessment of the prebiotic potential of Aloe vera mucilage and
its impact on the human microbiotaâ€, Food Function, vol. 6, no.
, pp 525-31, 2013.
Zanjani MAK, Tarzi BG, Sharifan A, Mohammadi N, Bakhoda H
and Madanipour MM., â€œMicroencapsulation of Lactobacillus casei
with calcium alginate-resistant starch and evaluation of survival
and sensory properties in cream-filled cakeâ€, African Journal of
Microbiology Research, vol. 6, pp. 5511-5517, 2012.
Homayouni A, Azizi A, Ehsani MR, Yarmand MS and Razavi SH.,
â€œEffect of microencapsulation and resistant starch on the
probiotic survival and sensory properties of synbiotic ice
creamâ€, Food Chemistry, vol. 111, pp. 50-55, 2008.
Bouhadir KH, Lee KY, Alsberg E, Damm KL, Anderson KW,
Mooney DJ., â€œDegradation of partially oxidized alginate and its
potential application for tissue engineeringâ€, Biotechnology
Progress, vol. 17, pp. 945â€“950, 2001.
Borchers A.T., Selmi C., Meyers F.J., Keen C.L., Gershwin M.E.,
â€œProbiotics and immunityâ€, Journal of Gastroenterology, vol. 44,
pp. 26â€“46, 2009.
Elizabeth C. Verna, â€œUse of probiotics in gastrointestinal
disorders: what to recommend?â€, Therapy Advance in
Gastroenterology, vol. 3, no. 5, pp. 307â€“319, 2010.
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