Study of Mg-Hydroxyapatite Composite with various composition of Hydroxyapatite which obtained From Cow Bones in Simulation Body Fluid (SBF)


  • Jojor Lamsihar Manalu
  • Bambang Soegijono University of Indonesia
  • Decky Jusiana Indrani


Keywords— Magnesium, hydroxyapatite from cow bone, composites


The objective of the present study was to synthesize biodegradable Mg-hydroxyapatite (Mg-HAp) composites for bone replacement. Hydroxyapatite (HAp) powders were synthesized from bioresources cow bone in a cost effective and ecofriendly way. Cow bone was converted to hydroxyapatite by a heat treatment method at different temperatures. Hydroxyapatite was characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM), Energy Dispersive X-Ray spectroscopy (EDX) and FT-IR. The results show a uniform distribution of HAp with a crystal size range of 400-800 nm. The Mg–HAp composites were prepared by mixing different amounts of HAp powders to Mg powder and finally the powder was pressed to make samples. The microstructure of Mg–HAp composite was examined by optical microscope (OM).The results show a uniform distribution of HAp particles along the boundary between matrix particles.. The relative densities of the composites initially increased with increase the amount of HA addition. The theoretical and experimental hardness of the composites are compared with  the addition of  3 to 9 wt%  HAp. Addition of HAp decreases the crystal size of the nanocomposites. The mechanical properties, i.e. hardness  is evidently increased with increasing HAp content up to 5 wt%.


Author Biography

Bambang Soegijono, University of Indonesia

Lecturere and researcher at Departement of Physics

University of Indonesia


Kannan B.M, Raman S.R.K. In vitro degradation and mechanical integrity of calcium containing magnesium alloys I modified Simulated Body Fluid: Biomaterials , 29, 14,2003

Staiger M P, Pietak A M, Huadmai J and Dias G . Magnesium and its alloys as orthopedic biomaterials: a review . Biomater : 27, 1728,2006

Witte F., Kaese V., H. HaferkamP, E. Switzer, A. Meyer-Lidenberg, C.J. Wirth, H. Windhagen. In Vivo Corrosion of Four Magnesium Alloys and The Associated Bone Response" : Biomaterials, 26,3557 , 2005

Witte F., Reinfenrath J., Muller P'P.,Crosstack H-A., Nellesen J., Bach F.W., Bormann D, Rudert M. ,,Cartilage Repair on Magnesium Scaffolds Used as a Subchondral Bone : Replacement". Mateialwissenschaft und Weksrffiechnik : 37 (6), 504-508', 2006

Yu G, Zhang E, Pan F, Yang K, : In vivo corrosion behavior of Mg-Mn-Zn alloy for bone implant application J. Biomed. Mater. Res. 83, 703, 2007

Song G, Corros. Sci., Recent progress in corrosion and protection of magnesium alloys, 49, 1696.2007

Jarcho M, Bolen C.H, Thomas M.B, Bobick J, Kay J F, Doremus R H., J. Mater. : Hydroxylapatite synthesis and characterization in dense polycrystalline form.Sci., 11, 2027. 1976

Khalil K. A, Kim S W, Dharmaraj N, Kim H Y., : Corrosion Passivation in Simulated Body Fluid of Magnesium/ Hydroxyapatite Nanocomposites Sintered by High Frequency Induction Heating Int. J. Appl. Ceram. Techol., 8, 523, 2011

Kim S W, Cockcroft S.L, Khalil K. A, Ogi K, A-structural Materials Properties microstructure and Processing Mater. Sci. Eng. A, 527, 4926, 2010

Khalil K. A, Kim S W, Kim H Y, Novel mechanism to improve toughness of the hydroxyapatite bioceramics using high-frequency induction heat sintering, Mater. Sci. Eng. A, 456, 368. 2007

X. Gu, W. Zhou, Y. Zheng, L. Dong, Y. Xi, D. Chai, A study on alkaline heat treated Mg–Ca alloy for the control of the biocorrosion rate. Mater. Sci..Eng. C 30, 827,832, 2010

Ooi, C. Y.; Hamdi, M.; Ramesh, S. Properties of hydroxyapatite produced by annealing of bovine bone. Ceram. Int. : 33, 1171-1177, 2007

Dachun, L.; Wei, C. Preparation and characterization of natural hydroxyapatite from animal hard tissues. Key Eng. Mat.: 342, 343, 2007

Ivankovic, H.; Gallego Ferrer, G.; Tkalcec, E.; Orlic, S.; Ivankovic, M. Preparation of highly porous hydroxyapatite from cuttlefish bone. J. Mater. Sci.-Mater. Med: 20, 1039-1046, 2009

Kim, S.; Park, P.; Kim, Y. Study on acute subcutaneous toxicity of hydroxyapatite sinter produced from tuna bone in Sprague—Dawly rats. Korean J. Life Sci.: 11, 97–10, 2001

Manalu J L, Soegijono B, Indrani D J, Characterization of Hydroxyapatite Derived from Bovine Bone Asian Journal of Applied Sciences : 3(4), 758-765, 2015




How to Cite

Manalu, J. L., Soegijono, B., & Indrani, D. J. (2016). Study of Mg-Hydroxyapatite Composite with various composition of Hydroxyapatite which obtained From Cow Bones in Simulation Body Fluid (SBF). Asian Journal of Applied Sciences, 4(4). Retrieved from




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