Effect of Micro-bubbles Ozone for Inactivation of <i>Escherichia coli </i>O157:H7 on Fresh-cut Pineapple cv. Phu Lae

Authors

  • A. Chuajedton The Graduate School Chiang Mai University, Chiang Mai 52000
  • H. Aoyagi Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba Ibaraki 305-8572
  • J. Uthaibutra Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400
  • K. Whangchai Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400

Keywords:

Escherichia coli, fresh-cut, micro-bubbles ozone, pineapple

Abstract

This study investigated the effect of micro-bubbles ozone (MBO) for inactivation of Escherichia coli O157:H7 on fresh-cut pineapple cv. “Phu Laeâ€. Fresh-cut pineapples were dip-inoculated with Escherichia coli O157:H7 (approximately 108 CFU/ml) and washed with MBO concentration of 0.14, 0.12, 0.06, and 0.03 mg O3/L at room temperature (28±1ºC) for 5, 10, 15 and 30 min, respectively ; then stored at room temperature (28±1ºC). The results showed that the population of Escherichia coli O157:H7 on fresh-cut pineapples washed with MBO for 10 min significantly reduced after storage for 2 days (less than 1 log10CFU/g) when compared to all treatments and the control (distilled water). Result from scanning electron microscope (SEM) obviously shown that less Escherichia coli O157:H7 in biofilms when compared to control. Therefore, the application of micro-bubbles ozone potentially enhance the ability of pathogenic control and prolonged shelf life of fresh-cut fruits

References

Office of Agricultural Economics. Statistical Export – import. Retrieved September 1, 2015, from

http://www.oae.go.th/oae_report/export_import/export_result.php

Jennylynd, B. J. & Ngarmsak, T. (2010). Processing of fresh-cut tropical fruits and vegetables: A technical guide.

Food and Agriculture Organization of the United Nations Regiolan Office for Asia and the Pacific Bangkok, 2010.

González, G. A., Ruiz-Cruz, S., Crua-Valenzuela, R., Rodríguez-Félix, A. and Wang, C. Y. 2004. Physiological and

quality changes of fresh-cut pineapple treated with antibrowning agents. Lebensmittel-Wissenschaft und-

Technologie 37: 369-376.

Nguyen-the, C. and Carlin, F. 1994. The microbiology of minimally processed fresh fruits and vegetables. Critcal

Review in Food Science and Nutrition 34(4): 371-401.

Puthmee, T. Matulaprungsan, B. and Kanlayanarat, S. 2010. Cutting styles of fresh-cut pineapple cv. Trad Seethong on quality after storage. Agricultural Science Journal 41(3/1)(Suppli.): 125-128.

Graham, D. M. 1997. Use of ozone for food processing. Food Technology 51: 72-75.

Xu, L. 1999. Use of ozone to improve the safety of fresh fruits and vegetables. Food Technology 53(10): 58–61, 63.

Hampson, B.C. and Fiori, S.R. 1997. Applications of ozone in food processing operations. Proceeding International

Ozone Association PAG, Lake Tahoe, Nev. 261-267.

Kim, J-G., Yousef, A-E. and Dave, S. 1999. Application of ozone for enhancing the microbiological safety and

quality of food: a review. Journal of Food Protection 62(9):1071-1087.

Kondo, F., Utoh, K., and Rostamibashman, M. 1989. Sterillizing effect of ozone water and ozone ice on various

microorganisms. Food Technology 53(10):58–61.

Takahashi, M., Chiba, K. and Li, P. 2007. Formation of hydroxyl radicals by collapsing ozone microbubbles under

strong acid conditions. The Journal of Physical Chemistry 111: 11443-11446.

Marui, T. 2013. An introduction to micro/nano-bubbles and their applications. Systemics, Cybernetics and

Informatics 11(4):68-73.

Li, P., Takahashi, M. and Chiba, K. 2012. Degradation of phenol by collapse of microbubbles. Chemosphere 75:

-1375.

Inatsu, Y., Kitakawa, T., Nakamura, N., Kawasaki, S., Nei, D., Bari, M. L. and Kawamoto, S. 2011. Effectiveness of

stable ozone microbubble water on reducing bacteria on the surface of selected leafy vegetables. Food Science and

Technology Research 17 (6): 479-485.

Fukumoto, Y., Hashizume, K. and Nishimura, Y. 2010. Development of supply system of microbubble ozonated

water in agriculture. Horticulture Environmental Biotechnology. 5 (1): 21-27.

Hoigne, J. and Bader, H. 1980. Bestimmung von Ozon und Chlordioxid im Wasser mit der Indigo-Methode. Vom

Wasser 55:261.

Zimmerman, W.B, Tesar, V., Butler, Simon, B. and Bandulasena, HC. H. 2015. Microbubble generation. (Institute

of Thermomechanics) Retrieved from ftp://tepeu.sisal.unam.mx/microbubbles/ZimmermanTesaratal

Reviewmicrobubblegeneration.pdf

Ozone. 1999. EPA Guidance Manual Alternative Disinfectants and Oxidant. Downloaded from http://water.epa.gov/lawsregs/rulesregs/sdwa/mdbp/upload_2001__12_mdbp_alter_chapt_3.pdf.

Jacek, M. 2012. Method for measuring ozone concentration in ozone-treated water. Przeglad elektrotechniczny

(electrical review) 88: 253-255.

Williams, R. C., Sumner, S. S. and Golden, A. A. 2004. Survival of Escherichia coli O157:H7 and Salmonella in

apple cider and orange juice as affected by ozone and treatment temperature. Journal of Food Protection

(11):2381-2386.

Keskinen, L. A., Burke, A. and Annous, B. A. 2009. Efficacy of chlorine, acidic electrolyzed water and aqueous

chlorine dioxide solutions to decontaminate Escherichia coli O157:H7 from lettuce leaves. International Journal of

Food Microbiology 132:134-140.

Anupunpisit, V., Thanomsub, S., Chanphetch, S., Watcharachaipong, T., Poonkhum, R. and Srisukonth, C. 2002.

Effects of ozone treatment on cell growth and ultrastructural changes in bacteria. The Journal of general and

applied microbiology 48:193-199.

Curtiellas, V., Gutiérrez, M., Sánchez, E., Fernández, I., Baluja, Ch., Bataller, M., Rodríguez, S. and Oncheta, O.

Characterization of E. coli cell lysis by ozone. Proceeding of the 17th Ozone World Congress, Strasbourg :

France.

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Published

2016-03-05

How to Cite

Chuajedton, A., Aoyagi, H., Uthaibutra, J., & Whangchai, K. (2016). Effect of Micro-bubbles Ozone for Inactivation of <i>Escherichia coli </i>O157:H7 on Fresh-cut Pineapple cv. Phu Lae. Asian Journal of Applied Sciences, 4(1). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/3586