Phyllogenetic Molecular Ectorhizosphere Bacteria from Medicinal Plant <i>Ageratum conyzoides L. </i>Employing Amplified Ribosomal DNA Restriction Analyses (ARDRA)
Keywords:
A. conyzoides, ARDRA, ectorhizosphere, phyllogeneticAbstract
The study on phyllogenetic molecular employing Amplified Ribosomal DNA Restriction Analyses (ARDRA) of ectorhizosphere bacteria from medicinal plant Ageratum conyzoides has been conducted. The aim of the research is firstly, to study restriction pattern of Hha1 or Msp1 in 16S rDNA resulting in vitro amplification from plasmid recombinant and secondly, to study phyllogenetic molecular from ectorhizosphere bacteria. The method was used chromosomal DNA isolation, in vitro amplification of 16S rDNA, insert verification applying Polymerase Chain Reaction (PCR) , restriction of amplicon of 16S rDNA with Hha1 or Msp1, and phyllogenetic construction employing MVSP 3.2. Restriction pattern showed genetic diversity of ectorhizosphere bacteria. Phyllogenetic analyses revealed endophyte bacteria has three big clusters. Shanon-Wiener diversity showed that ectorhizosphere bacteria have high diversity. Phyllogenetic revealed the genetic diversity of endophyte bacteria in A. conyzoides.
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