Comparative Study of Leuco Dyes as Reagents for Spectrophotometric Determination of Arsenic (â…¢)


  • Minori Kamaya Kogakuin University, 2665-1, Nakano-machi, Hachioji-city, Tokyo
  • Yusuke Ito
  • Yuki Otomura
  • Elena Ginatullina


arsenic(â…¢), leuco dyes, iodine, spectrophotometry


In this paper spectrophotometric determination arsenic (III) is described. The method is based on the two step reaction. First step is arsenic (Ⅲ) and iodate reaction in the acid medium of pH=1.1. In this step, iodine is liberated proportionally according to the amounts of arsenic (Ⅲ). Next step is the reaction of liberated iodine with leuco dyes in pH= 4-5. In this work, the leuco dyes was comparatively studied. The employed leuco dyes are malachite green (LMG), leuco crystal violet (LCV) and leuco berberin blue Ⅰ (LBB). The oxidized leuco dyes was determined by spectrophotometry. Beer’s law is obeyed in the range of 0.1-0.1μg mL-1 as arsenic. The molar coefficient for arsenic (Ⅲ) was around 4×104 l mol-1 cm-1 with each leuco dyes. In these leuco dyes, LBB was suitable as reagent because not influenced temperature, concentration of iodate and LBB, and rapid reaction time.




Mandal BK, Suzuki KT, “Arsenic round the world: a reviewâ€, Talanta, vol. 58, pp. 201-215, 2002.

Morita K, Kaneko E, “Spectrophotometric determination of arsenic in water samples based on micro particles formation of ethyl violet-molybdoarsenatâ€, Anal Sci, vol. 22, pp.1085-1089, 2006.

Dasgupta PK, Huiliang HL, Zhang GF, Cobb GP, “Photometric measurement of trace As(â…¢) and As(â…¤) in drinking waterâ€, Talanta, vol. 58, pp.153-164, 2002.

Ferrerira MA, Barros AA, “Determination of As(â…¢) and Arsenic(â…¤) in natural waters by cathodic stripping voltammetry at a hanging mercury drop electrodeâ€, Anal Chim Acta, vol. 459, pp.151-159, 2002.

Kopanicia M, Novotny L, “Determination of traces of arsenic(â…¢) by anoidic stripping voltammetry in solutions, natural waters and biological materialsâ€, Anal Chim Acta, vol. 368, pp. 211-218, 1998.

Bundelaska JM, Stafilov T, Appadjian S, “Direct analysis of natural waters for arsenic species by hydride generation atomic absorption spectrometryâ€, Int J Environ Anal Chem, vol.85, pp. 199-207, 2005.

Heitkemper DT, Vela NP, Stewart KR, Westphal CS, “Determination of total and speciated arsenic in rice by ion chromatography and inductively coupled plasma mass spectrometryâ€, J Anal At Spectrom, vol. 16, pp.299-306, 2001.

Boadu M, Osae EK, Golow AA., Serfor-Armah Y, Nyarko BJB, “Determination of arsenic in some water bodies, untreated ore and tailing samples at Konnongo in Ashanti region of Ghana and its surrounding town and villages by instrumental neutron activation analysisâ€, J Radioanal Nucl Chem, vol. 249, pp.581-585, 2001.

Gupta VK, Agarwal S, “PVC based 5,10,15,20-tetrakis (4-methoxyphenyl) porphyrinatocobalt(â…¡) membrane potentiometric sensor for arseniteâ€, Talanta, vol. 65, pp.730-734, 2005.

Gomez MM, Kovecs M, Palacios MA, Pizarro I, Camara C, “Effect of the mineralization method on arsenic determination in marine organisms by hydride generation atomic fluorescence spectroscopyâ€, Microchim Acta vol.150, 9-14, 2005.

Abdolmohammad-Zahen H, Jouyban A, Amini R, “Ultratrace determination of arsenic in water samples by electrothermal atomic absorption spectrometry after pre-concentration with Mg-Al-Fe ternary layered double hydroxide nano-sorbentâ€, Talanta, vol. 116, pp. 604-610, 2013.

Karayunlu S, Ay U, “Spectrophotometric determination of total inorganic arsenic with hexamethylene ammonium-hexamethylenedithiocarbamate in nonionic triton X-100 micellar mediaâ€, J Anal Chem, vol. 65, pp. 244-248, 2010.

Deepa K, Lingappa Y, “A simple spectrophotometric method for the determination of arsenic in industrial and environmental samples using 2,4-dihydroxy benzophenone-2-amino thiophenolâ€, Spectrochim.Acta, Part A, vol. 124, pp.102-107, 2014.

Cherian T, Narayama B, “A new spectrophotometric method for the determination of arsenic in environmental and biological samplesâ€, Anal Lett, vol. 38, pp.2207-2216, 2005.

Yuji S, Kato T, Nukatsuka I, Ohzeki K, “Spectrophotometric determination of arsenic(â…¢) based on solid phase extraction of the arsenic-APDC complex and the conversion to the copper complexâ€, Bunseki Kagaku, vol.52, pp. 1153-1158, 2003.

Pasha C, Narayana B, “Determination of arsenic in environmental and biological samples using toluidine blue or safranine O by simple spectrophotometric methodâ€, Bull Environ Contam Toxico, vol. l 81, pp. 47-51, 2008.

Revanasiddappa HD, Dayananda BP, Kumar TNK, “A sensitive spectrophotometric method for the determination of arsenic in environmental samplesâ€, Environ Chem Lett, vol. 5, pp.151-155, 2007.

Pillai A, Sunita G, Gupta VK, “A new system for the spectrophotometric determination of arsenic in environmental and biological samplesâ€, Anal Chim Acta, vol. 408, pp.111-115, 2000.

Agrawal O, Sunita G, Gupta VK, “A sensitive colorimetric method for the determination of arsenic in environmental and biological samplesâ€, J Chin Chem Soc, vol. 46, 641-645,1999.

Karayannis MI, Tzouwara-Karayanni SM, Hadjiioannou TP, “Kinetic study and analytical application of the iodate-arsenite reaction in strongly acidic solutionsâ€, Anal Chim Acta, vol. 70, pp. 351-357, 1974.




How to Cite

Kamaya, M., Ito, Y., Otomura, Y., & Ginatullina, E. (2015). Comparative Study of Leuco Dyes as Reagents for Spectrophotometric Determination of Arsenic (â…¢). Asian Journal of Applied Sciences, 3(1). Retrieved from