Rapid Detecion of N-Acetylneuraminic Acid from False Clownfish using HPLC-FLD for Symbiosis to Host Sea Anemone
Keywords:N-acetylneuraminic acid, false clownfish, HPLC-FLD, sea anemone
The symbiosis of false clownfish to its host sea anemone is unique as sea anemone is known to discharge toxin to approaching prey. This study aims to investigate by which biochemical property of the false clownfish mucus that enables the fish to adapt to the stinging tentacles of the sea anemone. N-acetylneuraminic acid (Neu5Ac) is a member of the sialic acid family that is secreted in the mucus of many marine and terrestrial organisms. It is important for cell recognition in endocrine regulation and cell immune system.Â Mucus samples were collected from sea anemone Heteractis magnifica and three fish species, Amphiprion ocellaris, Abudefduf sexfasciatus, and Thalassoma lunare.Â Samples were prepared byderivatization with thiobarbituric acid prior to rapid detection by HPLC-FLD.Â The principal result showed that false clownfish, A. ocellaris, significantly lacks Neu5Ac (1.636 mg/ml) as compared to other reef fish tested (50.433 mg/ml and 71.893 mg/ml respectively).Â As Neu5Ac is detected by the tentacles of sea anemone to trigger toxin discharge, it is concluded that the lack of Neu5Ac by false clownfish protects it from being stung.
Anderluh, G., & Macek, P. (2002).Cytolytic peptide and protein toxins from sea anemones (Anthozoa: Actiniaria). Toxicon, 40(2), 111â€“24.
Karthikayalu, S., Rama, V., & Venkatesan, R. (2010). Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica. The Journal of Venomous Animals and Toxins Including Tropical Diseases, 16(3), 514â€“518.
Mebs, D. (2009). Chemical biology of the mutualistic relationships of sea anemones with fish and crustaceans.Toxicon, 54(8), 1071â€“4.
Ricciardi, F., Boyer, M., & Ollerton, J. (2010). Assemblage and interaction structure of the anemonefish-anemone mutualism across the Manado region of Sulawesi, Indonesia. Environ. Biol. Fish.DOI: 10.1007/s10641-010-9606-0
Ollerton, J., Mccollin, D., Fautin, D. G., & Allen, G. R. (2007). Finding NEMO : nestedness engendered by mutualistic organization in anemonefish and their hosts. Proceedings of the Royal Society of London. Series B, Biological Sciences, 274 (February), 591â€“598.
Palaniappan, S. S., Azizah, M. N. S., & Yusuf, Y. (2003). Notes on the Occurrence of Amphiprion (Perciformes : Pomacentridae ) in Peninsular Malaysia. Asian Fisheries Sciences, 16, 235â€“240.
Mariscal, R. N. (1970). The nature of the symbiosis between Indo-Pacific anemone fishes and sea anemones. Marine Biology, 6(1), 58â€“65.
Holbrook, S. J., & Schmitt, R. J. (2004).Growth, reproduction and survival of a tropical sea anemone (Actiniaria): benefits of hosting anemonefish. Coral Reefs, 24(1), 67â€“73.
Porat, D. (2004). Effects of anemonefish on giant sea anemones: Ammonium uptake, zooxanthella content and tissue regeneration. Hydrobiologia, 530/531, 513â€“520.
Roopin, M., & Chadwick, N. E. (2009). Benefits to host sea anemones from ammonia contributions of resident anemonefish. Journal of Experimental Marine Biology and Ecology, 370(1-2), 27â€“34.
Szczebak, J. T., Henry, R. P., Al-Horani, F. A., & Chadwick, N. E. (2013). Anemonefish oxygenate their anemone hosts at night.The Journal of Experimental Biology, 216(Pt 6), 970â€“6.
Lubbock, R. (1980). Why are clownfishes not stung by sea anemnones?Proceedings of the Royal Society of London. Series B, Biological Sciences, 207(1166), 35â€“61.
Elliott, J. K., Mariscal, R. N., & Roux, K. H. (1994).Do anemonefishes use molecular mimicry to avoid being stung by host anemones? Journal of Experimental Marine Biology and Ecology, 179(1), 99â€“113.
Norton, R. S. (2009).Structures of sea anemone toxins. Toxicon, 54(8), 1075â€“88.
Ozacmak, V. H., Thorington, G. U., Fletcher, W. H., & Hessinger, D. A. (2001).N-acetylneuraminic acid (NANA) stimulates in situ cyclic AMP production in tentacles of sea anemone (Aiptasia pallida): possible role in chemosensitization of nematocyst discharge. The Journal of Experimental Biology, 204(Pt 11), 2011â€“2020.
Kimura, M., Hama, Y., Sumi, T., Asakawa, M., Rao, B. N., Horne, A. P., Nakagawa, H. (1994). Characterization of a deaminated neuraminic acid-containing glycoprotein from the skin mucus of the loach, Misgurnus anguillicaudatus.. The Journal of Biological Chemistry, 269(51), 32138â€“32143.
Sumi, T., Hama, Y., Nakagawa, H., Maruyama, D., & Asakawa, M. (2004).Purification and further characterization of a glycoprotein from the skin mucus of Japanese eels. Journal of Fish Biology, 64, 100â€“115.
Chen, H., Wang, P., Chen, Y., & Li, H. (2011).Determination of Total Sialic Acid Content in Human Milk by HPLC-FLD.é™ˆæµ·å¨‡, çŽ‹è, é™ˆè·ƒ, ç‰. é£Ÿå“ç§‘å¦, 32(16), 308â€“311.
Eddy, F. & Fraser, J. (1982). Sialic acid and mucus production in rainbow trout (Salmo gairdneri richardson) in response to zinc and seawater. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 73, 357-359.
Severi, E., Hood, D., & Thomas, G. (2007).Sialic acid utilization by bacterial pathogens. Microbiology, 153, 2817-2822.
Schlesinger, A., Zlotkin, E., Kramarsky-Winter, E., & Loya, Y. (2009). Cnidarian internal stinging mechanism.Biological Sciences / The Royal Society, 276(1659), 1063â€“1067.
Thorington, G. U., McAuley, V., & Hessinger, D. A. (2010). Effects of Satiation and Starvation on Nematocyst Discharge, Prey Killing, and Ingestion in Two Species of Sea Anemone. Biol. Bull., 219(2), 122â€“131.
Todaro, D. & Watson, G. (2012). Force-dependent discharge of nematocysts in the sea anemone Haliplanella luciae (Verrill).Biology Open, 1 (6), 582-587.
Krayesky, G., Sherry L., Mahoney, J. L., Kinler, K. M., Peltier, S., Calais, W., Allaire, K., & Watson, G. M. (2010). Regulation of spirocyst discharge in the model sea anemone, Nematostella vectensis. Marine Biology, 183-193.
Mebs, D. (1994).Anemonefish symbiosis: vulnerability and resistance of fish to the toxin of the sea anemone. Toxicon, 32 (9), 1059-1068.
Elliott, J. K., & Mariscal, R. N. (1997). Acclimation or innate protection of anemonefishes from sea anemones? Copeia, 1997(2), 284â€“289.
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