Variation of the <i>In Vitro</i> and Phenological Behaviours of 6 Cocoa Hybrids Using the Discriminant Model in the Second Year of the Study


  • Emmanuel Auguste Issali CNRA (Centre National de Recherche Agronomique)
  • Hervé Cedessia Kéassemon Koné
  • Deless Fulgence Thiémélé
  • Sangaré Abdourahamane


Promising hybrids, Culture media PCG, SCG and ED medium, Callogenesis, somatic embryogenesis, flowering level, Côte d’Ivoire


To analyse the variation of the in vitro and phenological behaviours, typological analyses were performed. Six newly promising cocoa hybrids known as L120-A2, L126-A3, L231-A4, L232-A9, L233-A4 and L330-A9 were used. Three culture media namely PCG1, PCG3 and PCG4, using DKW basal salt medium, only differing in hormonal concentration, were sown with staminodes and petals from these hybrids. SCA6 and C151-61 were included as controls. The 6 cocoa hybrids were monitored for 3 months at the end of which the callogenesis and embryogenesis variables were scored on each them. Collected data were processed via the Principal Component (PCA), Hierarchical Cluster (HCA) and Factorial Discriminant Analyses (FDA). Regarding the PCA, the flowering level, fructification level and leaves flush as well as number of embryogenic explants, embryos number yielded per embryogenic explant and embryogenesis percentage were found to be relevant. As for the FDA, only the flowering level, fructification level and number of callogenic explants were revealed to be pertinent. This postulates that the pertinentness seems to vary as a function of the analysis. Genotypes L120-A2 and L126-A3 expressed the highest callogenesis and flowering level compared to 4 others hybrids. They are half sibs with IMC67 as a common male parent. The line whose the equation is written Z = - 41.365 + 0.907*Nivflo discriminated the 3 clusters identified in the proportion of 100%. It allowed not only the describing of clusters, but also the predicting of the cluster of belonging of a new individual from its flowering level values. The aforementioned hybrids could be used to produce cocoa aroma, theobromin and cocoa butter from cell suspensions in bioreactors for biotechnology purposes. Such cultures should be achieved when flowering and fructification are high.

Author Biography

Emmanuel Auguste Issali, CNRA (Centre National de Recherche Agronomique)

Côte d'Ivoire is not represented among countries cited


Whitlock B. A., Bayer C., Baum D. A. Phylogenetic relationships and floral evolution of the Byttnerioideae

(″Sterculiacaea″ or Malvaceae s.l.) based on sequences of the chloroplast gene, ndhF. John V; Freudenstein Editeur.

System. Bot.. Volume 26 .Pp. 420-437, 2001.

ICCO. Yearly report 2004 / 2005. Copyright © 2006. The International Cocoa Organization (ICCO). Commonwealth

House, 1-19 New Oxford Street. Londres WC1A-1NU, Royaume-Uni. Pp. 12, 2005.

Anonyme, Rapport annuel de la zone franc. Banque de France. Eurosystème. Pp. 279, 2004.

ICCO. Célébration du cacao. Organisation Internationale du Cacao (ICCO). Publié par K. P. Parteners in Publishing

Ltd. Imprimé et relié par Interprint, (Malte). Pp. 115, 2000.

Mossu G. Le cacaoyer. Editeurs Lienhart & Cie, Aubenas d’Ardèche, France. ACCT-CTCAR. Pp. 159, 1990.

Ngoran J. A. K. Amélioration de la production du cacaoyer. Rapport Annuel d’Activités 1989, IRCC – Côte

d’Ivoire. Pp. 36-50, 1988.

Kofler, L., Croissance et développement des plantes. Gauthier Villar, Paris. Pp 234, 1969.

Tan C.L. and Furtek D.B. Development of an in vitro regeneration system for Theobroma cacao from mature

tissues. Plant Sci., Vol. 164. Pp. 407-412, 2003.

Issali A. E., Traoré A., Ngoran J. A. K., Koffi K. E., Sangaré A., Relationship between some phenological

parameters and somatic embryogenesis in Theobroma cacao L., J. of Crop Sci. and Biotech.

Vol. 11. Pp. 23-30, 2009.

Issali A. E., Traore A., Koffi K. E., N’goran K. A. J., Sangaré. Characterization of callogenic and

embryogenic of some genotypes of cocoa (Theobroma cacao L.,) under selection in Côte d’Ivoire, Biotech.

Vol. 7. Pp. 51-58, 2008.

Issali A. E. Climat, phénologie, génétique et embryogenèse somatique du cacaoyer. Publication de ma thèse

de Doctorat d’Université. Editions Universitaires Européennes (EUE), Parution 4 février 2011. Pp. 168, 2011a.

Issali A. E., Traore A., Minyaka E., Allou D., Sangare A., Diarrassouba N., Lékadou T. T., Toure M.,

Resemblance between somatic embryo-derived plantlets and plantlets bearing cotyledons of Theobroma cacao L., in

relation to their growth in the greenhouse using the typology. Am. J. Biotechnol. Mol. Sci, Vol. 1. Pp. 17-29, 2011b.

Issali A. E. Climate, phenology, genetics and embryogenesis in Theobroma cacao L., Lambert Academic

Publishing (LAP), doctoral dissertation defended at Cocody-Abidjan University, Côte d’Ivoire. Pp. 108-109, 2012.

Lachenaud, P., Eskes, A., N’Goran, J.A.K., Clement, D., Kebe , I., Tahi, M., Cilas, C. Premier cycle de

sélection recurrente en Côte d’Ivoire et choix des géniteurs du second cycle. In: Proceedings of the 13 th

International Cocoa Research Conference, 9–14 October 2000, Kota Kinabalu, Sabah, Malaysia, Pp. 9–14, 2001.

Maximova S. N., Alemanno L., Young A., Ferriere N., Traore A., and Guiltinan M. J., 2002. Efficiency, genotypic

variability and cellular origin of primary and secondary somatic embryogenesis of Theobroma cacao L., In Vitro

Cell. Dev. Biol. Plant, Vol. 38. Pp 252–259, 2002.

Li Z., Traoré A., Maximova S. and Guiltinan, M. J., 1998. Somatic embryogenesis and plant regeneration from

floral explants of cocoa (Theobroma cacao L.) using Thidiazuron, In vitro Cell Dev. Biol. Plant, Vol. 34. Pp. 293-

, 1998.

Heller, R. Esnault, R. Lance, C. Physiologie végétale. Tome 2. Développement. 5e Edition mise à jour et

augmentée. Masson. Paris-Milan-Barcelone. Pp. 315, 1995.

Augé R. Beauchesne G., Bocon-Gibod J., Decourtye L., Digat B., Galandrin J. C., Minier R., Morand J. C., Vidalie

H. La culture in vitro et ses applications horticoles. Edition revue. J. B. Baillière. Technique et documentation

(Lavoisier). Pp. 143, 1984

Web site references : web site proposing solution regarding multivariate analysis.




How to Cite

Issali, E. A., Koné, H. C. K., Thiémélé, D. F., & Abdourahamane, S. (2014). Variation of the <i>In Vitro</i> and Phenological Behaviours of 6 Cocoa Hybrids Using the Discriminant Model in the Second Year of the Study. Asian Journal of Applied Sciences, 2(6). Retrieved from

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