Variation of the <i>In Vitro</i> and Phenological Behaviours of 6 Cocoa Hybrids Using the Discriminant Model in the Second Year of the Study
Keywords:Promising hybrids, Culture media PCG, SCG and ED medium, Callogenesis, somatic embryogenesis, flowering level, CÃ´te dâ€™Ivoire
To analyse the variation of the in vitro and phenological behaviours, typological analyses were performed. Six newly promising cocoa hybrids known as L120-A2, L126-A3, L231-A4, L232-A9, L233-A4 and L330-A9 were used. Three culture media namely PCG1, PCG3 and PCG4, using DKW basal salt medium, only differing in hormonal concentration, were sown with staminodes and petals from these hybrids. SCA6 and C151-61 were included as controls. The 6 cocoa hybrids were monitored for 3 months at the end of which the callogenesis and embryogenesis variables were scored on each them. Collected data were processed via the Principal Component (PCA), Hierarchical Cluster (HCA) and Factorial Discriminant Analyses (FDA). Regarding the PCA, the flowering level, fructification level and leaves flush as well as number of embryogenic explants, embryos number yielded per embryogenic explant and embryogenesis percentage were found to be relevant. As for the FDA, only the flowering level, fructification level and number of callogenic explants were revealed to be pertinent. This postulates that the pertinentness seems to vary as a function of the analysis. Genotypes L120-A2 and L126-A3 expressed the highest callogenesis and flowering level compared to 4 others hybrids. They are half sibs with IMC67 as a common male parent. The line whose the equation is written Z = - 41.365 + 0.907*Nivflo discriminated the 3 clusters identified in the proportion of 100%. It allowed not only the describing of clusters, but also the predicting of the cluster of belonging of a new individual from its flowering level values. The aforementioned hybrids could be used to produce cocoa aroma, theobromin and cocoa butter from cell suspensions in bioreactors for biotechnology purposes. Such cultures should be achieved when flowering and fructification are high.
Whitlock B. A., Bayer C., Baum D. A. Phylogenetic relationships and floral evolution of the Byttnerioideae
(â€³Sterculiacaeaâ€³ or Malvaceae s.l.) based on sequences of the chloroplast gene, ndhF. John V; Freudenstein Editeur.
System. Bot.. Volume 26 .Pp. 420-437, 2001.
ICCO. Yearly report 2004 / 2005. Copyright Â© 2006. The International Cocoa Organization (ICCO). Commonwealth
House, 1-19 New Oxford Street. Londres WC1A-1NU, Royaume-Uni. Pp. 12, 2005.
Anonyme, Rapport annuel de la zone franc. Banque de France. EurosystÃ¨me. Pp. 279, 2004.
ICCO. CÃ©lÃ©bration du cacao. Organisation Internationale du Cacao (ICCO). PubliÃ© par K. P. Parteners in Publishing
Ltd. ImprimÃ© et reliÃ© par Interprint, (Malte). Pp. 115, 2000.
Mossu G. Le cacaoyer. Editeurs Lienhart & Cie, Aubenas dâ€™ArdÃ¨che, France. ACCT-CTCAR. Pp. 159, 1990.
Ngoran J. A. K. AmÃ©lioration de la production du cacaoyer. Rapport Annuel dâ€™ActivitÃ©s 1989, IRCC â€“ CÃ´te
dâ€™Ivoire. Pp. 36-50, 1988.
Kofler, L., Croissance et dÃ©veloppement des plantes. Gauthier Villar, Paris. Pp 234, 1969.
Tan C.L. and Furtek D.B. Development of an in vitro regeneration system for Theobroma cacao from mature
tissues. Plant Sci., Vol. 164. Pp. 407-412, 2003.
Issali A. E., TraorÃ© A., Ngoran J. A. K., Koffi K. E., SangarÃ© A., Relationship between some phenological
parameters and somatic embryogenesis in Theobroma cacao L., J. of Crop Sci. and Biotech.
Vol. 11. Pp. 23-30, 2009.
Issali A. E., Traore A., Koffi K. E., Nâ€™goran K. A. J., SangarÃ©. Characterization of callogenic and
embryogenic of some genotypes of cocoa (Theobroma cacao L.,) under selection in CÃ´te dâ€™Ivoire, Biotech.
Vol. 7. Pp. 51-58, 2008.
Issali A. E. Climat, phÃ©nologie, gÃ©nÃ©tique et embryogenÃ¨se somatique du cacaoyer. Publication de ma thÃ¨se
de Doctorat dâ€™UniversitÃ©. Editions Universitaires EuropÃ©ennes (EUE), Parution 4 fÃ©vrier 2011. Pp. 168, 2011a.
Issali A. E., Traore A., Minyaka E., Allou D., Sangare A., Diarrassouba N., LÃ©kadou T. T., Toure M.,
Resemblance between somatic embryo-derived plantlets and plantlets bearing cotyledons of Theobroma cacao L., in
relation to their growth in the greenhouse using the typology. Am. J. Biotechnol. Mol. Sci, Vol. 1. Pp. 17-29, 2011b.
Issali A. E. Climate, phenology, genetics and embryogenesis in Theobroma cacao L., Lambert Academic
Publishing (LAP), doctoral dissertation defended at Cocody-Abidjan University, CÃ´te dâ€™Ivoire. Pp. 108-109, 2012.
Lachenaud, P., Eskes, A., Nâ€™Goran, J.A.K., Clement, D., Kebe , I., Tahi, M., Cilas, C. Premier cycle de
sÃ©lection recurrente en CÃ´te dâ€™Ivoire et choix des gÃ©niteurs du second cycle. In: Proceedings of the 13 th
International Cocoa Research Conference, 9â€“14 October 2000, Kota Kinabalu, Sabah, Malaysia, Pp. 9â€“14, 2001.
Maximova S. N., Alemanno L., Young A., Ferriere N., Traore A., and Guiltinan M. J., 2002. Efficiency, genotypic
variability and cellular origin of primary and secondary somatic embryogenesis of Theobroma cacao L., In Vitro
Cell. Dev. Biol. Plant, Vol. 38. Pp 252â€“259, 2002.
Li Z., TraorÃ© A., Maximova S. and Guiltinan, M. J., 1998. Somatic embryogenesis and plant regeneration from
floral explants of cocoa (Theobroma cacao L.) using Thidiazuron, In vitro Cell Dev. Biol. Plant, Vol. 34. Pp. 293-
Heller, R. Esnault, R. Lance, C. Physiologie vÃ©gÃ©tale. Tome 2. DÃ©veloppement. 5e Edition mise Ã jour et
augmentÃ©e. Masson. Paris-Milan-Barcelone. Pp. 315, 1995.
AugÃ© R. Beauchesne G., Bocon-Gibod J., Decourtye L., Digat B., Galandrin J. C., Minier R., Morand J. C., Vidalie
H. La culture in vitro et ses applications horticoles. Edition revue. J. B. BailliÃ¨re. Technique et documentation
(Lavoisier). Pp. 143, 1984
Web site references
http://www.lemoal.org/spss/ : web site proposing solution regarding multivariate analysis.
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