Optimization of Culture Conditions for the Highest Lipid Production from some Oleaginous Fungi for Biodiesel Preparation

Authors

  • Thanaa Hamed Ali Microbial Chemistry Dept., Genetic Engineering and Biotechnology Division, National Research Center, Tahrir St., 12622 Dokki, Cairo
  • Dina H. El-Ghonemy

Keywords:

Biodiesel, Lipid, Oleaginous fungi, Trichoderma viride, optimization

Abstract

Ten different fungal strains belonging to filamentous fungi were evaluated for their abilities to produce lipid. An oleaginous fungus strain Trichoderma viride-314 was grown the fastest and gave comparable high lipid content up to 20 %. Culture conditions were optimized for oil production by the above fungus in the medium supplemented  with  different  carbon  sources,  different  concentrations  of  the  best carbon source, different pH, temperature and incubation period. The experimental results  showed  that PD liquid  nutrient  medium  supported  relatively lipid accumulation with 50 g L-1  for dextrose concentration at the fifth day of growth.

 

Neither yeast extract nor sugar cane molasses supplement exhibits any significant effects on lipid production.   In addition, 28°C, 5.0, static conditions were the favorable for growth  temperature,  pH  value  of  cultivation  medium,  respectively.  The  GC/Ms analysis indicated that the n-hexane lipid fraction of T. viride was mainly composed of

30.01% Palmitoleic acid (C16:1), 23% Linoleic Acid (C18:2), 13% Linolenic acid (C18:3)  and  about  8%  Erucic  acid  (C22:1).  Furthermore,  the  GC  analysis  also showed that the total saturated fatty acids represented 8.3% while the value of the total unsaturated was 81.74%. These results giving possibility to use such this fungus strain to produce SCO in a low cost medium from economic point of view.

References

• Adachi D, Hama S, Numata T, Nakashima K, Ogino C, Fukuda H, Kondo A. 2011. Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production. Bioresource Technology. 102(12): 6723-6729.

• Amanullah A, McFarlane CM, Emery AN, Nienow AW. 2001. Scale-down model to simulate spatial pH variations in large-scale bioreactors. Biotechnol Bioeng.73:390–399.

• Azocar L, Ciudad G, Heipieper HJ, Navia R. 2010. Biotechnological processes for biodiesel production using alternative oils. Applied Microbiology and Biotechnology. 88(3): 621-636.

• Carlile MJ, Watkinson SC, Graham WG. 2001. The fungi. 2nd ed. San Diego (California): Academic Press.

• Chatzifragkou A, Fakas S, Galiotou-Panayotou M, Aggelis G, Papanicolaou S. 2011. Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi. Eur J Lipid Sci Technol. 112(9): 1048-1057. .

• Chen XF, Huang C, Xiong L, Chen X, Chen Y, Maa LL. 2012. Oil production on waste waters after butanol fermentation by oleaginous yeast Trichosporon coremiiforme. Bioresour Technol. 118:594–597.

• Chisti Y. 2007. Biodiesel from microalgae, Biotechnol. Adv. 25:294–306.

• Christie WW, Han X. 2010. Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis (4th edition), 446 pages (Oily Press, Bridgwater, U.K. and Woodhead Publishing Ltd, Cambridge, U.K.)

• Chtzifragkou S, Fakas M, Galiotou-Panayotou M, Komaitis G, Aggelis, Papanikolaou S. 2010. Commercial sugars as substrates for lipid accumulation by Cunninghamella echinulata and Mortierella isabellina fungi. Eur J Lipid Sci Technol. 112:1048–1057.

• Devi P, D'souza L, Kamat T, Rodrigues C, Naik CG. 2009. Batch culture fermentation of Penicillium chrysogenum and a report on the isolation, purification, identification and antibiotic activity of citrinin. Indian J Mar Sci. 38(1): 38.

• Difco, Manual. (1972). "Difco Manual of dehydrated culture, media and reagents" 9th ED.:245. Difco Laboratories, Detroit, Michigan, USA.

• Economou CN, Aggelis G, Pavlou S, Vayenas DV. 2011. Single cell oil production from rice hulls hydrolysate. Biores Technol. 102:9737–9742.

• Halim R, Danquah MK, Webley PA. 2012. Extraction of oil from microalgaen for biodiesel production: areview. Biotechnology Advances. 30:709–732.

• Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A. 2008. Microalgal triacylglycerols as feedstock for biofuel production: perspectives and advances. Plant J. 54: 621–639.

• Khot M, Kamat S, Zinjarde S, Pant A, Chopade B, RaviKumar A. 2012. Single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel. Microbial Cell Factories. 11:1-13.

• Kirrolia A, Bishnoi NR, Sing R. 2012. Effect of shaking, incubation temperature, salinity and media composition on growth traits of green microalgae Chlorococcum sp. J. Algal Biomass Utln . 3 (3):46–53.

• Kumar SP, Banerjee R. 2013. Optimization of lipid enriched biomass production from oleaginous fungus using response surface methodology Indian Journal of Experimental Biology. 51: 979-983.

• Li CH, Cervantes M, Springer DJ, Boekhout T, Ruiz-Vazquez RM, Torres-Martinez SR, Heitman J, Lee SC. 2011. Sporangiospore size dimorphism is linked to virulence of Mucor circinelloides. PLoS Pathogen. 7(6):e1002086.

• Lilly VG, Barnett HL. 1951. Physiology of fungi. New York: McGraw Hill Book Company Inc. 464.

• Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M. 2009. Biodiesel production from oleaginous microorganisms. Renewable Energy. 34 (1):1-5.

• Mishra S K, Suh WI, Farooq W, Moon M, Shrivastav A, Park M. S. Ji-Won Yang .2014. Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method. Bioresource Technology. 155:330–333.

• Pant D, Adholeya A. 2009. Nitrogen removal from biomethanated spentwash using hydroponic treatment followed by fungal decolorization. Env Eng Sci. 26:559–65.

• Pant D, Adholeya A. 2010. Development of a novel fungal consortium for the treatment of molasses distillery wastewater. Environmentalist. 30:178–182.

• Park WS, Murphy PA, Glatz BA. 1990. Lipid metabolism and cell composition of the oleaginous yeast Apiotrichum curvatum grown at different carbon to nitrogen ratios. Can J Microbiol. 36:318–26.

• Peng W, Huang C, Chen X, Xiong L, Chen X, Chen Y. MaL. 2013. Microbial conversion of waste water from butanol fermentation to microbial oil by oleaginous yeast Trichosporon dermatis. Ren Energy. 55:31–34.

• Ramanaiah SV, Venkata Mohan S, Sarma PN. 2007. Adsorptive removal of fluoride from aqueous phase using waste fungus (Pleurotus ostreatus 1804) biosorbent: kinetics evaluation. Ecol Eng. 31:47–56.

• Ramos MJ, Fernández CM, Casas A, Rodríguez L, Perez Ã. 2009. Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour Technol. 100:261–268.

• Ratledge C. 2004. Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie. 86(11):807–815.

• Sakuradani E, Ando A, Ogawa J, Shimizu S. 2009. Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding. Appl Microbiol Biotechnol. 84(1):1-10.

• Somashekar D, Venkateshwaran G, Srividya C, Krishnanand, Sambaiah K, Lok esh BR. 2001. Efficacy of extraction methods for lipid and fatty acid composition from fungal cultures. World J Microbiol Biotechnol . 17:317–320.

• Subramaniam R, Dufreche S, Zappi M, Bajpai R. 2010. Microbial lipids from renewable resources: production and characterization. J Ind Microbiol Biotechnol. 37:1271–1287.

• Venkata Subhash G, Venkata Mohan S. 2011. Biodiesel production from isolated oleaginous fungi Aspergillus sp. using corncob waste liquor as a substrate. Bioresour Technol. 102:9286–9290.

• Venkata Subhash G, Venkata Mohan S. 2014. Lipid accumulation for biodiesel production by oleaginous fungus Aspergillus awamori: Influence of critical factors. Fuel 116: 509–515.

• Vicente G, Bautista LF, Rodriguez R, Gutierrez FJ, Sadaba I, Ruiz-Vazquez RM, Torres-Martinez S, Garre V. 2009. Biodiesel production from biomass of an oleaginous fungus. Biochem Eng J. 48:22–27.

• Vicente GM, Martínez J, Aracil A. 2004. Integrated biodiesel production: a comparison of different homogeneous catalysts systems, Bioresour. Technol. 92:297–305.

• Wynn JP, Hamid AA, Li Y, Ratledge C. 2001. Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina. Microbiology. 147(10):2857-2864.

• Yousuf A, Sannino F, Addorisio V, Pirozzi D. 2010. Microbial conversion of olive oil mill waste waters into lipids suitable for biodiesel production. J Agri Food Chem. 58: 8630–8635.

• Zinoviev S, Müller-Langer F, Das P, Bertero N, Fornasiero P, Kaltschmitt M, Centi G, Miertus S. 2010. Next-generation biofuels: Survey of emerging technologies and sustainability issues. Chemistry and sustainability, energy and materials. 3(10):1106-1133.

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Published

2014-10-15

How to Cite

Ali, T. H., & El-Ghonemy, D. H. (2014). Optimization of Culture Conditions for the Highest Lipid Production from some Oleaginous Fungi for Biodiesel Preparation. Asian Journal of Applied Sciences, 2(5). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/1762

Issue

Vol. 2 No. 5 (2014): October 2014

Section

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