Optimization of Culture Conditions for the Highest Lipid Production from some Oleaginous Fungi for Biodiesel Preparation
Keywords:Biodiesel, Lipid, Oleaginous fungi, Trichoderma viride, optimization
Ten different fungal strains belonging to filamentous fungi were evaluated for their abilities to produce lipid. An oleaginous fungus strain Trichoderma viride-314 was grown the fastest and gave comparable high lipid content up to 20 %. Culture conditions were optimized for oil production by the above fungus in the medium supplemented Â with Â different Â carbon Â sources, Â different Â concentrations Â ofÂ the Â best carbon source, different pH, temperature and incubation period. The experimental resultsÂ showed Â that PD liquidÂ nutrient Â medium Â supported Â relatively lipid accumulation with 50 g L-1 Â for dextrose concentration at the fifth day of growth.
Neither yeast extract nor sugar cane molasses supplement exhibits any significant effects on lipid production.Â Â In addition, 28Â°C, 5.0, static conditions were the favorable for growth Â temperature, Â pH Â value Â of Â cultivation Â medium, Â respectively. Â The Â GC/Ms analysis indicated that the n-hexane lipid fraction of T. viride was mainly composed of
30.01% Palmitoleic acid (C16:1), 23% Linoleic Acid (C18:2), 13% Linolenic acid (C18:3) Â and Â about Â 8%Â Erucic Â acid Â (C22:1). Â Furthermore, Â theÂ GC Â analysis Â also showed that the total saturated fatty acids represented 8.3% while the value of the total unsaturated was 81.74%. These results giving possibility to use such this fungus strain to produce SCO in a low cost medium from economic point of view.
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