Studies on Cryopreservation of Human Oocytes using Verification Technique

El Ghareeb A., Hamdi H., Mansour R., Abdel-Fatah M.

Abstract


This study aims to extend the applications of oocytes cryopreservation into the clinical practice of ART. Using the vitrification method applied on 676 human oocytes divided into four groups based on their maturation stage, presence of corona cells and the time to start cryopreservation. Group (A) includes 177 MII oocytes, fully denuded and vitrified 3-5 hours after pick up, (B) includes 194 GV/MI oocytes fully denuded and vitrified 3-5 hours after pick up, (C) contains 103 oocytes vitrified 24 hours from retrieval at the MII stage and fully denuded and (D) contains 202 oocytes vitrified with the corona cells at the MII stage 3-5 hours after pick up. Survivability is determined one hour after thawing then the survived oocytes undergo ICSI procedure. Group (A), after thawing 161 oocytes (91%) survived, 111 (69%)  got fertilized, and only 38 (34%) developed into blastocysts. Group (B), 167 oocytes (86%) survived then 60 (36%) matured to MII, and 40 (67 %) got fertilized, then 11 embryos (28%) developed into blastocyst. Group C, 67 oocytes survived (65%), 44 got fertilized (66%) and 8 embryos (18%) reached blastocyst stage. Group D, 188 oocytes survived (93%), 126 got fertilized (67%) and 39 embryos developed into blastocyst (31%). The vitrification method of oocytes is very successful in preserving viability and fertilization .The presence or removal of the corona cells before vitrification didn't affect the results. Delaying vitrification 24 hours significantly lowered them. Vitrifying GV oocytes resulted in a reasonable survival rate; however the fertilization rate was very low.


Keywords


Oocytes cryopreservation, vitrification, IVF, ICSI.

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References


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