Biochemical Characterization of Alpha-amylase from the Digestive Fluid of Larvae of the Rhino Beetle, Oryctes owariensis


  • Due Ahipo Edmond
  • Bernard Assielou
  • Yolande Dogoré Digbeu
  • Hubert Kouassi Konan
  • Soumaila Dabonné
  • Jean Parfait Eugène N’guessan Kouadio


Oryctes owariensis, alpha-amylase, Enzyme, monomeric protein, Energy production, Starch saccharification


Alpha-amylase was purified from the digestive fluid of larvae of the Rhino Beetle (Oryctes owariensis) by chromatography on ion-exchanges and hydrophobic interaction columns. The preparation was shown to be homogeneous on polyacrylamide gel. The enzyme was purified 260.20-fold to a specific activity of 128.50 (U/ mg of protein) and an overall yield of 26.22 %. SDS-PAGE revealed a single polypeptide of 53.8 ± 1.2 kDa, thus indicating apparent homogeneity of the final enzyme preparation. Gel filtration chromatography showed that the enzyme was a 50.4 ± 2.2 kDa monomeric protein. The purified enzyme exhibited pH and temperature optima at 7.0 and 55°C, respectively. The alpha-amylase was stable at 37 °C and its pH stability was in the range of 6.6-7.6. This enzyme exhibited a high affinity towards soluble starch with Km values of 0.6 ±0.04. The alpha-amylase activities were stimulated by Ca2+, K+, Mg2+ and Na+ and were inhibited by EDTA, Fe2+ and Cu2+. The analysis of hydrolytic products after soluble starch hydrolysis by alpha-amylase revealed that maltose, maltotriose and maltotetraose were the major products. This indicated that this enzyme can be classified as the alpha-amylase (the endoamylase). From a physiological point of view, alpha-amylase play a fundamental role in energy production for this insect larvae. Consequently, the digestive tract of this larva could be a good source of alpha-amylase for starch saccharification.

Author Biography

Due Ahipo Edmond

UFR STA, department of biochemestry


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How to Cite

Edmond, D. A., Assielou, B., Digbeu, Y. D., Konan, H. K., Dabonné, S., & Kouadio, J. P. E. N. (2014). Biochemical Characterization of Alpha-amylase from the Digestive Fluid of Larvae of the Rhino Beetle, Oryctes owariensis. Asian Journal of Applied Sciences, 2(3). Retrieved from




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