Efficacy study of Indigenous Probiotics for Treatment of Acute Diarrhea in Infants: A Double- Blind, Placebo-Controlled Study

Authors

  • Amara Hassan
  • M. Nawaz Chaudhry
  • Barbara Rasco

Keywords:

indigenously isolated probiotics, acute diarrhea, multistrain probiotics, Double- blind, Placebo-controlled clinical trial, recovery time

Abstract

Double- blind, Placebo-controlled clinical trial was conducted to study the efficacy of three indigenous probiotics in the treatment of acute diarrhea in infants. Pateints were catedorized by ages ( birth to 6 months and 6 to 12 months) and observed daily for number of stools per day, vomiting episodes, loss of weight, and recovery days from diarrhea. In first category, the majority of patients were normalized in 16±2.7 days after intaking Lactobacillus-formula, while took 11±1.9 days in the case of Bifido-fromula. Minimum number of days were calculated for the recovery of patients in the Mixed-formula (04±1.2 days). While the controlled group recovered in 21±4 days. In second category,  a significantly late recovery response was observed in the controlled group (28±1.3 days) while Mixed formula again proved to be the best formula with only 08±2.2 recovery days in major cases with significant value of 3.4±1.4 mean number of stool per day, 0.9±0.3mean vomiting episodes per infant, and 0.5±1.1mean loss in weight per infant. The results revealed that these  indigenously isolated probiotics, especially in the mixed multistrain cultures are effective in treatment of acute diarrhea in infants.

References

Joint, F. WHO Expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Córdoba, Vol. 1, No. 4, 2001.

Alam, N. H. and Ashraf, H. Treatment of infectious diarrhea in children. Pediatric Drugs, Vol. 5, No. 3,pp. 151-165, 2003.

van Zanten, S. V. ACP Journal Club: probiotics improve symptoms in adults with the irritable bowel syndrome. Annals of internal medicine, Vol. 153, No.6 , pp. JC3-7 , 2010.

Szajewska, H., Setty, M., Mrukowicz, J. and Guandalini, S. Probiotics in gastrointestinal diseases in children: hard and not-so-hard evidence of efficacy. Journal of pediatric gastroenterology and nutrition, Vol.42,No. 5, pp. 454-475, May 2006.

Silva, M., Jacobus, N., Deneke, C. and Gorbach, S. Antimicrobial substance from a human Lactobacillus strain. Antimicrobial Agents and Chemotherapy, Vol. 31, No. 8, pp. 1231-1233, 1987.

Wilson, K. H. and Perini, F. Role of competition for nutrients in suppression of Clostridium difficile by the colonic microflora. Infect. Immun.,Vol. 56, No. 10, pp. 2610-2614, 1988.

Rigothier, M., Maccario, J. and Gayral, P. Inhibitory activity of saccharomyces yeasts on the adhesion ofEntamoeba histolytica trophozoites to human erythrocytes in vitro. Parasitol. Res., Vol. 80,No. 1, pp. 10-15, 1994.

Szajewska, H. Advances and limitations of evidence-based medicine--impact for probiotics. Annals of nutrition & metabolism, Vol. 57, pp. 6-9, 2010.

Majamaa, H., Isolauri, E., Saxelin, M. and Vesikari, T. Lactic acid bacteria in the treatment of acute rotavirus gastroenteritis. Journal of pediatric gastroenterology and nutrition, Vol. 20,No. 3, pp. 333-338, 1995.

Isolauri, E. Dietary modification of atopic disease: Use of probiotics in the prevention of atopic dermatitis. Current allergy and asthma reports, Vol. 4, No. 4, pp. 270-275, Jul 2004.

Davidson, G. P. and Butler, R. N. Probiotics in pediatric gastrointestinal disorders. Current opinion in pediatrics, Vol. 12, No. 5, pp. 477-481, 2000.

Michail, S. K., Stolfi, A., Johnson, T. and Onady, G. M. Efficacy of probiotics in the treatment of pediatric atopic dermatitis: a meta-analysis of randomized controlled trials. Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 101,No. 5, pp. 508-516, 2008.

D'Souza, A. L., Rajkumar, C., Cooke, J. and Bulpitt, C. J. Probiotics in prevention of antibiotic associated diarrhoea: meta-analysis. Bmj,Vol. 324,No. 7350, pp. 1361, 2002.

Tankanow, R., Ross, M., Ertel, I., Dickinson, D., McCormick, L. and Garfinkel, J. A double-blind, placebo-controlled study of the efficacy of Lactinex in the prophylaxis of amoxicillin-induced diarrhea. The Annals of pharmacotherapy, Vol. 24, No. 4, pp. 382-384, 1990.

Thomas, M. R., Litin, S. C., Osmon, D. R., Corr, A. P., Weaver, A. L. and Lohse, C. M. Lack of Effect of< i> Lactobacillus GG on Antibiotic-Associated Diarrhea: A Randomized, Placebo-Controlled Trial. vol. 76,No. 9, pp. 883-9, 2001.

Chouraqui, J.-P., Van Egroo, L.-D. and Fichot, M.-C. Acidified milk formula supplemented with Bifidobacterium lactis: impact on infant diarrhea in residential care settings. Journal of pediatric gastroenterology and nutrition,vol. 38, No. 3, pp. 288-292, 2004.

Saavedra, J. M. and Yolken, R. H. Re: Clinical efficacy of probiotics: review of the evidence with focus on children. Journal of pediatric gastroenterology and nutrition, Vol. 44,No. 4 , pp. 510-501, 2007.

Masood, M. I., Qadir, M. I., Shirazi, J. H. and Khan, I. U. Beneficial effects of lactic acid bacteria on human beings. Crit. Rev. Microbiol., Vol. 37, No. 1 pp. 91-98, 2011.

Dicks, L. M. and Botes, M. Probiotic lactic acid bacteria in the gastro-intestinal tract: health benefits, safety and mode of action. Beneficial microbes, vol. 1, No. 1, pp. 11-29, 2010.

Allen, S. J., Martinez, E. G., Gregorio, G. V. and Dans, L. F. Probiotics for treating acute infectious diarrhoea. Cochrane database of systematic reviews, vol. 11, pp. CD003048, 2010.

Roller, M., Clune, Y., Collins, K., Rechkemmer, G. and Watzl, B. Consumption of prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis has minor effects on selected immune parameters in polypectomised and colon cancer patients. The British journal of nutrition, Vol. 97, No. 4, pp. 676-684, 2007.

Roller, M., Rechkemmer, G. and Watzl, B. Prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis modulates intestinal immune functions in rats. The journal of Nutrition, vol. 134, No. 1, pp. 153-156, 2004.

Moro, G., Minoli, I., Mosca, M., Fanaro, S., Jelinek, J., Stahl, B. and Boehm, G. Dosage-related bifidogenic effects of galacto-and fructooligosaccharides in formula-fed term infants. Journal of pediatric gastroenterology and nutrition, Vol. 34, No. 3, pp. 291-295, 2002.

Ghisolfi, J., Roberfroid, M., Rigo, J., Moro, G. and Polanco, I. Infant formula supplemented with probiotics or prebiotics: never, now, or someday? Journal of pediatric gastroenterology and nutrition, Vol. 35, No. 4, pp. 467-468, 2002.

Watson, D. Immunological functions of the mammary gland and its secretion-comparative review. Australian Journal of Biological Sciences, Vol. 33, No. 4, pp. 403-422, 1980.

Xu, J., Dennehy, P., Keyserling, H., Westerman, L., Wang, Y., Holman, R., Gentsch, J., Glass, R. and Jiang, B. Serum antibody responses in children with rotavirus diarrhea can serve as proxy for protection. Clin. Diagn. Lab. Immunol., Vol. 12,No. 2, pp. 273-279, 2005.

Uruakpa, F., Ismond, M. and Akobundu, E. Colostrum and its benefits: a review. Nutrition Research, Vol. 22, No. 6, pp. 755-767, 2002.

Nagao, A., Nero, F. D., Arslanian, C. and Carneiroâ€Sampaio, M. Elevated Levels and Different Repertoire Profile of Colostral Antiâ€LPS Antibodies May Have a Significant Role in Compensating Newborn Immunity. Scand. J. Immunol., Vol. 53,No. 6, pp., 602-609, 2001.

Downloads

Published

2014-06-21

How to Cite

Hassan, A., Chaudhry, M. N., & Rasco, B. (2014). Efficacy study of Indigenous Probiotics for Treatment of Acute Diarrhea in Infants: A Double- Blind, Placebo-Controlled Study. Asian Journal of Applied Sciences, 2(3). Retrieved from https://www.ajouronline.com/index.php/AJAS/article/view/1227

Issue

Section

Articles