In vitro Plant Regeneration from Hypocotyl and Cotyledon Explants of BARI SARISHA-13 (Brassica napus L.)
Keywords:BARI-13, in vitro regeneration, PGRs
AbstractThe aim of the present study was to develop an efficient in vitro regeneration system for BARI sarisha-13 (Brassica napus L.) where hypocotyls and cotyledonary petioles obtained from in vitro grown seedlings were used as explants for indirect regeneration. Single and combinatorial effects of plant growth regulators (PGRs) and additives were observed. Varying concentrations of hormones was found to change efficiency of callus, shoot and root formation.Â Increasing 2, 4- dichlorophenoxyacetic acid (2,4-D) from 0.5 mgl-1 to above andÂ 6, benzyl amino purine (BAP) concentration from 2 mg-1 to 3 mg-1 and NAA from 0.1 mg-1 to 0.5 mg-1 decreased callus and shoot formation, respectively. Roots were obtained when naphtalene acetic acid (NAA) was used. Effects of kinetin, AgNO3, casein hydrolysate (CH), proline, indole 3-butyric acid (IBA), etc were also seen in different stages of regeneration. Percentage and photographs of responsive explants was taken after 24 days from inoculation.
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