Virulence Factors and Prevalence of Udder´s Pathogens in Dairy Cows during the Peripartal Period

Authors

  • Zuzana Farkašová Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy, Košice, Slovakia
  • František Zigo Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy, Košice, Slovakia
  • Zuzana Lacková Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy, Košice, Slovakia

DOI:

https://doi.org/10.24203/ajafs.v9i6.6816

Keywords:

Dairy Cows, Peripartal Period Mastitis, Biofilm, DNAse

Abstract

Data presented in this study described the prevalence of udder pathogens and presence of virulence factors in staphylococci isolated from mastitis in dairy cows. The practical part of study was realized in five different dairy herds of Slovak spotted cattle breed located in Eastern Slovakia with conventional (non-organic) farming. At the beginning of lactation (during first two months after calving) were comprehensively investigated 960 cows from monitored herds. The comprehensive examination included clinical examination, sensory analysis of milk from fore stripping of each udder quarter, followed by assessment of the California mastitis test (CMT) and laboratory analyses of bacteria pathogens. Screening the health status of the mammary gland revealed that 314 cows (32.7%) had positive CMT score (1-3) for one or more quarters. Out of 230 infected milk samples, representing 24.0% of all dairy cows examined, were the most commonly isolated staphylococci (59.1% of positive findings), followed by E. coli (11.3%), streptococci Str. uberis (9.1%), Str. agalactiae (3.4%), and enterococci (6.1%). From 136 isolates of coagulase negative stafylococci (98 isolates) and S. aureus (38 isolates) were detected some virulence factors such as production of hemolysins (lysines ß and δ), gelatinase, biofilm, and hydrolyze of DNA. Isolated S. aureus, S. chromogenes and S. warneri had the most numerous representation of detected virulence factors, as demonstrated by the increased incidence of clinical forms of mastitis compared to less virulent strains.

References

Čobirka M, Tančin V, Slama P. 2020.” Epidemiology and Classification of Mastitis”. Animals. vol. 10, p. 2212.

orti S, Sicher D, Regli W, Stephan R. 2003. “Current data on antibiotic resistance of the most important bovine mastitis pathogens in Switzerland”. Schweiz Arch. Tierheilkd. vol. 145, pp. 571-575.

Dufour S, Labrie J, Jacques M. 2019. “The Mastitis Pathogens Culture Collection”. Microbiol Resour Announc. vol. 8, no. 15: e00133-19.

El-Diasty, M., Talaat, H., Atwa, S., Elbaz, E., Eissa, M. 2019. Occurrence of Coagulase-negative Staphylococcal mastitis in dairy cows. Mansoura Veterinary Medical Journal. vol. 23. p. 35-39. DOI: 10.35943/mvmj.2019.23.207

Fredheim E.G.A, Klingenberg C, Rodhe H, Frankenberger S, Gaustad P, Flaegstad T, Sollid J.E. 2009. “Biofilm formation by Staphylococcus haemolyticus”. J. Clin Microbiol. vol. 47, pp. 1172-80.

Fox L.K, Gay J.M. 1993.” Contagious mastitis”. The Veterinary clinics of North America. Food Animal. Vol. 9, pp. 475-87.

Gentilini E, Denamiel G, Betancor A, Rebuelto M, Fermepin M.R, De Torrest RA. 2002. “Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis in Argentina”. J. Dairy Sci., vol. 85, pp. 1913-1917.

Haveri M, Roslöf A, Pyörälä S. 2007. “Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics”. J Appl Microbiol. vol. 103, pp. 993-1000.

Hiko A. 2019. “Isolation, DNase-cross-Coagulase test and antimi-crobial resistance test on Staphylococcus along beef abattoir line in Addis Ababa Ethiopia”. Ethiop. Vet. J., vol. 23, no.1, pp. 90 - 110.

Holko I, Tančin V, Vršková M, Tvarožková K. 2019. “Prevalence and antimicrobial susceptibility of udder pathogens isolated from dairy cows in Slovakia”. Journal of Dairy Research. pp. 1 – 4.

Idriss S.E, Tančin V, Foltýs V, Kirchnerová K, Tančinová D, Vršková M. 2013. “Relationship between mastitis causative pathogens and somatic cell count in milk of dairy cows”. Slovak Journal of Food Sciences, vol. 7, no. 1, pp. 207-212.

Isaac P, Bohl L.P, Breser M.L, Orellano M.S, Conesa A, Ferrero M.A, Porporatto C. 2017. “Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens”. Vet Microbiol. vol. 207, pp. 259-266.

Kabrt M. (2013). “Aplikovaná statistika: Test chí-kvadrát nezávislosti v kontingenční tabulce”. [online]. Dostupné z: http://www.milankabrt.cz/testNezavislosti/

Marques V.F, da Motta C.C, Soares B.S, de Melo D.A, de Oliveira C.S.H, Coelho I.S, Santos H.B, de Souza M.M.S. 2017. “Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis”. Brazilian Journal of Microbiology. vol. 48, no 1, pp. 118-124.

Melchior M.B, Vaarkamp H, Fink-Gremmels J. 2006. “Comparative assessment of the antimicrobial susceptibility of Staphylococcus aureus isolates from bovine mastitis in biofilm versus planktonic culture”. Journal of Veterinary Medicine B, Infectious Diseases and Veterinary Public Health, vol. 53, pp. 326–332.

Moniri R, Dastehgoli K, Akramian A. 2007. “Increasing resistant CNS in bovine clinical mastitis”s. Pakistan J Biolog Sci. vol. 10, no. 15, pp. 2465-2469.

Monistero V, Graber H.U, Pollera C, et al. 2018. Staphylococcus aureus Isolates from Bovine Mastitis in Eight Countries: Genotypes, Detection of Genes Encoding Different Toxins and Other Virulence Genes. Toxins (Basel). Vol. 10, no. 6, p. 247.

Nascimento J.S, Fugundes P.C, Brito A.V, Dos Santos K.R, Bastos M.C. 2005. “Production of bacteriocins by coagulase-negative staphylococci involved in bovine mastitis”. Veterinary Microbiology, vol. 106, pp. 61-71.

National mastitis council. 2001. National Mastitis Council Recommended Mastitis Control Program. Natl. Mastitis Counc. Ann. Mtg. Proc., Reno, NV. Natl. Mastitis Council, Inc., Verona, WI. 408 p. ISBN 978-0-309-06997-7.

Notcovich S, DeNicolo G, Flint S.H, et al. 2018. “Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand”. Vet Sci. vol. 5, no. 1, p. 8.

Otto M. 2004. “Virulence factors of the coagulase-negative staphylococci”. Front. Biosci. vol. 9, pp. 841-863.

Otto M. 2018. “Staphylococcal Biofilms”. Microbiol Spectr. vol. 6, no. 4, p. 1-26.

Pyörälä S, Taponen S. 2009. “Coagulase-negative staphylococci - Emerging mastitis pathogens. Veterinary Microbiology, vol. 134, no. 1-2, p. 3-8. https://doi.org/10.1016/j.vetmic.2008.09.015

Supre K, Haesebrouck F, Zadoks R.N, Vaneechoutte M, Piepers S, De Vliegher S. 2011. “Some coagulase-negative Staphylococcus species affect udder health more than others”. J. Dairy Sci., vol. 94, pp. 2329-2340.

Tančin V. 2013. “Somatic cell counts in milk of dairy cows under practical conditions”. Slovak Journal of Animal Science, vol. 46, no. 1, pp. 31-34.

Tančin V, Mikláš Š, Čobirka M, Uhrinčať M, Mačuhová L. 2020. “Factors affecting raw milk quality of dairy cows under practical conditions”. Slovak Journal of Food Sciences, vol. 14, pp. 744–749.

Tvarožková K, Tančin V, Holko I., Uhrinčať M, Mačuhová L. 2019. “MASTITIS IN EWES: somatic cell counts, pathogens and antibiotic resistance”. Journal of Microbiology, Biotechnology and Food Sciences. vol. 9, no. 3, pp. 661-670

Tvarožková K, Tančin V, Uhrinčať M, Hleba L, Mačuhová L. 2020. “Mastitis pathogens and somatic cell count in ewes milk”. Slovak Journal of Food Sciences Vol. 14, pp. 164-169.

Vasiľ M, Elečko J, Zigo F, Farkašová Z. 2012. “Occurrence of some pathogenity factors in coagulase negative staphylococci isolated from mastitis milk in dairy cows”. Slovak Journal of Food Sciences. vol. 6, no. 2, pp. 60-63.

Vasiľ M, Farkašová Z, Elecko J, Illek J, Zigo F. 2017. “Comparison of biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis strains isolated from sheep milk using three diagnostic methods”. Polish J. of Vet. Sci. Vol. 20, pp. 795-801.

Vinodkumar K., Neetha, N, Ashok S, Suchithra S, Justin D, Radhik S. 2017. “Genotypic and phenotypic β-lactam resistance and presence of PVL gene in Staphylococci from dry bovine udder”. PLOS ONE. vol. 12. e0187277.

Zigo F, Sasáková N, Gregová G, Výrostková J, Ondrašovičová S. 2020. “Effects of Using an Alternative Bedding Composition on the Levels of Indicator Microorganisms and Mammary Health in Dairy Farm Conditions”. Agriculture, vol. 10, p. 245.

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Published

2021-12-31

How to Cite

Farkašová, Z. ., Zigo, F., & Lacková, Z. . (2021). Virulence Factors and Prevalence of Udder´s Pathogens in Dairy Cows during the Peripartal Period. Asian Journal of Agriculture and Food Sciences, 9(6). https://doi.org/10.24203/ajafs.v9i6.6816

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