Isolation and molecular analysis of SRBSDV isolates infecting rice in Vietnam

Authors

  • Thanh Tran Ngoc 1International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India And Agricultural Genetic Institute, Hanoi, Vietnam
  • Hoang Anh Ta Plant Protection Research Institute, Hanoi, Vietnam
  • Vinh Do Nang Agricultural Genetic Institute, Hanoi, Vietnam
  • Neeti Sanan-Mishra Plant Molecular Biology Group International Center for Genetic Engineering and Biotechnology, New Delhi

Keywords:

Fijivirus, Vietnam, Rice, Sequence analysis, Evolution

Abstract

Southern rice black-streaked dwarf virus (SRBSDV) is a newly described member of the sub-group 2 in the genus Fijivirus, family Reoviridae. SRBSDV genome has 10 segments encoding 13 opened reading frames (ORFs). In this study we cloned and sequenced 6 ORFs from SRBSDV isolates collected from 3 provinces in Vietnam. The sequence analysis suggested that SRBSDV populations in North Vietnam are closely related to those in South China. The viral isolates from Nghe-An and Quang-Tri provinces of Vietnam were closer to each other as compared to the Quang-Ngai province suggesting that the virus seems to be evolving while spreading in a southern direction.  Moreover the analysis revealed the presence of DNA binding domains in gene 5.2 indicating that it may be playing an important role in the viral life cycle.

Author Biographies

Thanh Tran Ngoc, 1International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India And Agricultural Genetic Institute, Hanoi, Vietnam

Plant Molecular Biology Group
International Center for Genetic Engineering and Biotechnology, New Delhi

Hoang Anh Ta, Plant Protection Research Institute, Hanoi, Vietnam

Plant Protection Research Institute,

Hanoi, Vietnam

Vinh Do Nang, Agricultural Genetic Institute, Hanoi, Vietnam

Agricultural Genetic Institute,

Hanoi, Vietnam

Neeti Sanan-Mishra, Plant Molecular Biology Group International Center for Genetic Engineering and Biotechnology, New Delhi

Staff Research Scientist

Plant Molecular Biology Group

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Published

2013-12-13

How to Cite

Ngoc, T. T., Ta, H. A., Nang, V. D., & Sanan-Mishra, N. (2013). Isolation and molecular analysis of SRBSDV isolates infecting rice in Vietnam. Asian Journal of Agriculture and Food Sciences, 1(5). Retrieved from https://www.ajouronline.com/index.php/AJAFS/article/view/609

Issue

Vol. 1 No. 5 (2013): December 2013

Section

Articles

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