Effect of Extraction Methods on Chemical and Physical Properties of <i>Aloe Vera</i> (<i>Aloe Barbadensis</i> Miller) Polysaccharides Fraction: Liguid Gel and Powders
Keywords:Aloe vera, extraction methods, acemannan, protein, polysaccharides, sugar
The aim of this study was to evaluate the influence of extraction methods on chemical and physical properties of Aloe vera polysaccharides. The study was conducted on two commercial products: Aloe vera powder and an extract liquid of whole leaf. The kinetics of hydrolysis is carried out on the Aloe vera products. Hot, cold extraction with water and boiled ethanol extraction were carried out to obtain polysaccharide fractions (A1, A2, A3, and A4). The molecular weights of each fraction were determined. Proteins, galacturonic acid and sugars were quantified. Results showed that approximately, 25% of sugars were present in Aloe vera powder. The best extraction method were cold extraction (pH 5.3, 25Â°C, 4h) which showed the higher extraction yields (69.4Â±0.1%) in polysaccharide (Poly) A, than other extraction methods. Interestly, results showed a decrease of molecular weights, molecular number, and protein contents from 150 to 30 kDa, from 97 to 29 kDa and from 4.9Â±0.1 to 0.00% respectively with polysaccharides fractionment methods. Moreover, the total sugar content increases in polysaccharide fraction: 29.2Â±0.1%, 76.6Â±0.1% and 93.4Â±0.4% for Poly A, A1 and A2 respectively. The highest sugar content were observed in Poly A3 â‰ˆ 97.8Â±1.5% probably glucomannan, with 77.3Â±6.5% of mannose, 18.7Â±2.8% of glucose. The data suggest that the fractionment methods could lead to product the purified polysaccharide which could be use for nutritional, biological and medicinal properties.
Joshi SP (1997). Chemical constituents and biological activity of Aloe barbadensis-A review. Journal of Medicinal and Aromatic plante Science 20: 768-773.
Yagi A, Hine N, Asai M, Nakazawa M, Tateyama Y, Okamura N, Fujioka T, Mihashi k, Shimomura K (1998). Tetrahydroanthracene glucosides in callus tissue from Aloe barbadensis leaves. Phytochemistry 47(7), 1227-1270.
Dagne E, Daniel B, Alvaro V and Ben-Erik V W (2000). Chemistry of Aloe Species. Current Organic Chemistry; 1055- 1078.
Haller JS (1990). A drug for all seasons, medical and pharmacological history of aloe. Bulletin of the New York Academy of Medicine 66:647-659.
Eshun K, He Q (2004). .Aloe vera: A valuable ingredient for the food, pharmaceutique and cosmetique industries-A review.Critical Reviews in food Science and Nutrition 44(2), 91-96.
Chow J., Williamson D.A., Yates K. M., Goux W.J. (2005). Chemical characterization of the immunomodulating polysaccharide of Aloe vera L. Carbohydrate Research 340, 1131â€“1142
Van Wyk B-E.; Smith, G. (1996). Guide to the Aloes of South Africa. Briza Publications, Pretoria, South Africa, 614.
Adesuyi AO, Awosanya OA, Adaramola FB and Omeonu AI (2012). Nutritional and Phytochemical Screening of Aloe barbadensis. Current Research of Journal Biological Science 4(1): 4-9.
Emmanuel Morin (2008). Aloe vera (L.) Brurm.f. : Aspects pharmacologiques et chimiques. ThÃ¨se dâ€™Etat en pharmacie, UniversitÃ© de Nantes, France. 224 pages.
Owoade F.M., Adeoye G.O. (2016). Nutritive value and nutrient uptake of Aloe vera L. Burm F. grown in beach sand as nnfluenced by nutrient application. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) 11 : 37-42
Hassan SW, Umar RA, Ladan MJ, Nyemike P, Wasagu RS. Lawal M, Ebbo AA (2007). Nutritive value, phytochemical and antifungal properties of pergularia tomentosa L. (Asclepiadaceae). International Journal of Pharmacology 4 : 334â€“340
Femenia A., SaÂ´nchez E. S., Simal S., RosselloÂ´C. (1999b). Compositional features of polysaccharides from Aloe vera (Aloe barbadensis Miller) plant tissues. Carbohydrate Polymers 39(2), 109â€“117
Mandal G, Ghosh R, Das A (1983). Characterisation of polysaccharides of Aloe barbadensis Miller: Part IIIâ€”structure of an acidic oligosaccharide. Indian Journal of Chemistry 22B: 890â€“893.
Ni Y, Turner D, Yates KM, Tizard I (2004). Isolation and characterization of structural components of Aloe vera L. leaf pulp. International Immunopharmacology 4: 1745â€“1755
Reynolds T, Dwec AC (1999) Aloe vera leaf gel: a review update. Journal of Ethnopharmacology 68: 3-37
Mandal G, Das A (1980). Structure of the D-galactan isolated from Aloe barbadensis Miller. Carbohydrate Research 86 (2): 247â€“257.
AOAC (1995). Official Methods of analysis of the Association of Official Agricultural Chemist (16th Ed.), edited by P. Cunniff, AOCS International, Gaithersburg, MD.
BIPEA (1976). Recueil des mÃ©thodes d'analyse des communautÃ©s europÃ©ennes. Bureau Interprofessionnel d'Ã‰tudes Analytiques, Gennevilliers. France. 140 p.
Albersheim, P., Nevins, D.J., English, P.D., Karr, A. (1967). â€œA method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatographyâ€. Carbohydrate Research 5, 340-345.
Blakeney AB, Harris PJ, Henry RJ, Stone BA (1983). A simple and rapide preparation of alditol acetates for monosaccharide analysis. Carbohydrate Research 113, 291-299.
Garna H, Mabon N, Wathelet B, Paquot M (2004). New method for hydrolysis and chromatographic analysis of pectin neutral sugar chains. Journal of Agricultural and Food chemistry 52 : 4652- 4659.
Garna H, Mabon N, Nott k, Wathelet B, Paquot M (2006). Kinetic of hydrolysis of pectin galaturonic acid chains and quantification by ionic chromatographiy. Journal of Food chemistry 96: 477- 484.
Bozzi A, Perrin C, Austin S, Arce V F (2006). Quality and authenticity of commercial aloe vera gel powders. Food Chemistry 103(1), 22-30.
Femenia A, Rigby NM, Selvendran RR, Waldron KW (1999a). Investigation of the occurrence of pectic-xylan-xyloglucan complexes in the cell wall of stem tissues. Carbohydrate Polymers 39(2), 151-164.
Di-Scala K, Vega-GÃ¡lvez A, Ah-Hen K, NuÃ±ez-Mancilla Y, Tabilo-Munizaga G, PÃ©rez-Won M, Giovagnoli C. (2013). Chemical and physical properties of aloe vera (Aloe barbadensis Miller) gel stored after high hydrostatic pressure processing. Food Science et Technology 33 (1), 1-8.
MuÃ±oz OM, Leal X, Quitral V and Cardemil L. (2015). Extraction, Characterization and Properties of the Gel of Aloe Vera (Aloe barbadensis Miller) Cultivated in Chile. Medicinal and Aromatic Plants 4 : 199
Femenia A, Garcia-pascual P, Simal S, Rossello C (2003). Effects of heat treatment and dehydration on bioactive polysaccharide acemannan and cell wall polymers from Aloe barbadensis Miller. Carbohydrate Polymers 51(4), 397-405.
Miranda M., Hector M., Katia R., Antonio V.G. (2009). Influence of temperature on the drying kinetic, physicochemical properties, and antioxidant capacity of Aloe vera (Aloe barbadensis Miller) gel. Journal of Food Engineering, v. 91, n. 2. 297-304.
Leung MYK, Liu C, Zhu LF, Hui YZ, Yu B, Fung KP (2004) Chemical and biological characterization of a Polysaccharide biological response modifier from Aloe vera L. var chinensis (Haw) Berg. Glycobiology 14(6): 501-510.
Salinas C (2012). Characterization of fructans in Aloe barbadensis Miller leaves under water stress. PhD thesis Faculty of Science, University of Chile.
Xu CL, Pranovich A, Hemming J, Holmbom B, Albrecht S, et al. (2009) Hydrolytic stability of water-soluble spruce O-acetyl galactoglucomannans. Holzforschung 63: 61-68.
Vega A, Nevenka G, Ampuero C, DÃaz LN, Lemus RM (2005). El Aloe vera (Aloe barbadensis Miller) as a component of functional OOD. Revista chilena de Nutricion 32: 208-214.
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