Changes on Antioxidant Metabolites and Enzymes in Soybean Inoculated and Treated with Fungicides

María Caridad Nápoles, Luis Miguel Mazorra, Carlos Bártoli, Gustavo González-Anta

Abstract


Nodules formed in roots of leguminous plants, as result of their interaction with rizobia bacteria may generate oxidative stress, due to high respiratory level and the reducing conditions required to fix nitrogen and the leghemoglobinautoxidation process. To avoid oxidative stress nodules need to maintain a high antioxidant activity and therefore they could synthesize antioxidant metabolites which would react with reactive oxygen species (ROS) during symbiosis and biological nitrogen fixation. However, the knowledge about the antioxidant system in soybean plants inoculated with induced Bradyrhizobiumjaponicum is limited. In this work, it was demonstrated that a treatment of soybean seeds with rhizobium bacteria induces nodules formation and this induction was linked with a higher oxidized state of ascorbic acid. Also, it was observed that the use of fungicidefavors nodulation but provokes a further oxidation of ascorbic acid. However, the observed increase of ascorbic acid-recycling enzymes could not be enough to counteract ascorbic acid oxidation. Taken together, our results suggest that an oxidative stress could occur during nodulation and that infected soybean plants could have a degree of tolerance to oxidation.


Keywords


Ascorbic acid, nodulation, antioxidants, soybean

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References


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