Molecular Characterization of <i>Fusarium oxysporum</i> isolates from Egypt using IGS Sequencing
Keywords:Fusarium oxysporum, intergenic spacer region IGS, sequencing, tandem repeats
Twenty isolates of Fusarium oxysporum were collected from different regions of Egypt and characterized using intergenic spacer (IGS) of the ribosomal DNA gene. The identified isolates were compared using sequences analyses on formae speciales level. The length of the amplified IGS region using universal primers CNL12 and CNS1 were about 2.6 kb in all F. oxysporum isolates. For specificity, oligonucleotide primers PNFo and PN22 produced a single DNA fragment of about 1.7 kb for each of the twenty isolates. Sequence cluster analysis using the neighbor-joining method showed that the twenty isolates divided into two clusters, cluster 1 contains isolates from El Fayoum and the remaining isolates on the other cluster. On the whole, there was no clear correlation between geographic origin or plant host and the sequence of IGS region. The results demonstrated the important role for utilization the IGS region as a molecular marker for the rapid and reliable diagnosis, identification and characterization of F. oxysporum pathogen in solanaceous crops in Egypt.
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