Antifungal Activity and Seasonal Variation of Green Alga (Ulva lactuca ) Extracts

Rasha E. Selim, Soad M. Ahmed, Saad R. El-Zemity, Sami Sh. Ramses, Yasser T. A. Moustafa

Abstract


The present study was undertaken to explore the inhibitory effect of seaweed extracts of Ulva lactuca (green alga) with five solvents (methanol, Ethanol, Methylene chloride, chloroform and hexane) and water at different concentrations of (100, 200, 250, 500, 1000, 1500, 2000, 4000 8000 ppm) along with control against the mycelial growth of Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Alternaria solani, Phytophthora infestanse and Botrytis cinerea by poison food technique. Result revealed that extract of Methanol showed the highest antifungal activity against all the tested fungi. While water extract showed a moderate antifungal activity against all the tested plant pathogenic fungi. Phytophthora infestans was most sensitive than the other tested fungi to all solvent extracts of Ulva lactuca. The results also showed that the highest antifungal activity was recorded in winter season while the lowest one was in summer. GC-MS analysis of methanolic extract of Ulva lactuca was also recorded.


Keywords


Ulva lactuca (green alga), antifungal activity, seasonal variation, Chemical analyses, Gas chromatography- mass spectrometry

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Mohana, D. C., Prasad, P., Vijaykumar, V., and Raveesha, K. A. (2011). Plant extracts effect on Seed-borne pathogenic fungi from seeds of paddy grown in southern India. Journal of Plant Protectection Research, 51(2): 101-106.

Demirel, Z., Yilmaz-Koz, F., Karabay-Yavasoglu, U., Ozdemir, G., and Sukatar, A. (2009). Antimicrobial and antioxidant activity of brown algae from the Aegean Sea. Journal of Serbian Chemical Society,74 (6): 619-628.

Jiménez, E., Dorta, F., Medina, C., Ramírez, C., Ramírez, I. and Cortés, H. (2011) Anti-Phytopathogenic Activities of Macro-Algae Extracts. Mar.Drug 9:739-756.

Mayer, A. M. S., Rodríguez, A. D., Taglialatela-Scafati, O. and Fusetani, N. (2013). Marine Pharmacology in 2009–2011: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, and Antiviral Activities; Affecting the Immune and Nervous Systems, and other Miscellaneous Mechanisms of Action. Marine Drugs. 11, 2510 – 2573.

Chandía, N. P., and Matsuhiro, B. (2008). Characterization of a fucoidan from Lessonia vadosa (Phaeophyta) and its anticoagulant and elicitor properties. Int. J. Biol. Macromol. 42, 235–240.

Chakraborty, K., Lipton, A. P., Paulraj, R., Chakraborty, R. D. (2010a) Guaiane sesquiterpenes from seaweed Ulva fasciata Delile and their antibacterial properties. Eur J Med Chem 45: 2237-2244.

Samanta, S. (2011). Activity of selected Algal Extracts against two fungal pathogens of Tea: Pestalotiopsis theae and Hypoxylon serepens. M. Sc. Thesis, Faculty of engineering and Technology. Srm University.

Kamble, S. M., Rokde, A. U., and Ashok M. Chavan, A. M. (2012). Antifungal activity of algal extracts against plant pathogenic fungi. International Multidisciplinary Research Journal. 2(3):23-24.

Ambika, S. and Sujatha, K. (2014). Comparative studies on brown, red and green alga seaweed extracts for their antifungal activity against Fusarium oxysporum f.sp. udum in Pigeon pea var. CO (Rg)7 (Cajanus cajan (L.) Mills.). Journal of Biopesticides. 7(2):167-176.

Ambika, S. Sujatha, K. (2015). Antifungal activity of aqueous and ethanol extracts of seaweeds against sugarcane red rot pathogen (Colletotrichum falcatum). Scientific Research and Essays. Vol. 10(6), pp. 232-235.

Kong, F., Mao, Y., Cui, F., Zhang, X. and Gao, Z. (2011) Morphology and Molecular Identification of Ulva Forming Green Tides in Qingdao China. J Ocean University of China 10: 73-79.

Amsler, C. D., (2008) Algal Chemical Ecology. Springer, USA.

Chakraborty, K., Lipton, A.P., Paulraj, R., Viajayan, K. K. (2010b) Antibacterial labdane diterpenoids of Ulva fasciata Delile from south western coast of the Indian Peninsula. Food Chem 119: 1399-1408.

Gamal, A. E., (2010). Biological importance of marine algae. Saudi Pharma J, 18: 1-25.

Messyasz, B. and Rybak, A. (2010) Abiotic factors affecting the development of Ulva sp. (Ulvophyceae, Chlorophyta) in freshwater ecosystems. Aquat Ecol 45: 75-87.

Awad, N. E. (2000) Biologically Active Steroid from the Green Alga Ulva lactuca. Phytother Res 14: 641-643.

Flodin, C. and Whitfield, F. B. (1999) 4-Hydroxybenzoic acid: a likely precursor of 2,4,6-tribromophenol in Ulva lactuca. Phytochem 51: 249-255.

Abou-ElWafa G. M. (2005). M. Sc. Thesis, Faculty of Science, Mansoura University, Mansoura (Egypt).

Hassan, S. and Ghareib, H. (2009) Bioactivity of Ulva lactuca L. acetone extract on germination and growth of lettuce and tomato plants. African Journal of Biotechnology Vol. 8 (16), pp. 3832-3838.

Flora, G. and Rani, S. M. V. (2012). An approach towards control of blast by foliar application of seaweed concentrate. Science Research Reporter 2(3): 213-217.

Zambonelli, A., Zechini D`Aulerio, A., Bianchi, A. and Albasini, A. (1996): Effects of essential oils on phytopathogenic fungi in vitro. J. Phytopathology 144, 491-494.

Harlapur, S. I., Kulkarni, M. S., Wali, M. C., and Srikantkulkarni, H. (2007). Evaluation of plant extracts, bio-agents and fungicides against Exserohilum turcicum causing Turcicum leaf blight of Maize. Journal of Agricultural Science, 20(3):541-544.

Finney D. J. (1971) Probit analysis, 3rd Edn. Cambridge University Press, Cambridge, UK, pp 1–333.

Cluzet, S. C., Torregrosa, C., Jacquet, C., Lafitte, J., Fournier, L., merci, S., Salamagne, S., Briand,X., Esquerre-Tugaye, M. T. and Dumas, B. (2004) . Gene expression profiling and protection of Medicago Truncatula against a fungal infection in response to an elicitor from green algae Ulva spp. Plant Cell and Environment. 27, 917–928.

Aruna, P., Mansuya, P., Sridhar, S., Suresh, J. and Babu, S. (2010). Pharmacognostical and antifungal activity of selected seaweeds from Gulf of Mannar region. Recent Res. Sci. Technol. 2, 115–119.

Amany, D. A., (2013). Evaluation the toxicity of some natural products as pesticides M.SC thesis thesis,faculty of Agric .Damanhur University.

Sahayaraj, K., Rajesh, S., Asha, A. and Rathi, J. M. (2012). Marine algae for the cotton Pest and disease management. In:Conference Proceedings of the International conference on Agriculture, Science and Engineering (ICASE2012), Vol. 1.

Asnad, A. and Tanver, A. (2014) Screening of potential seaweeds against Fusarium species isolated from fruits and vegetables in Baluchistan, Pakistan. International Journal of Biosciences,4 (3): 131-138.

Abd El-Baky, H. H., Farouk, K., Baz, E. L. and Baroty, G. S. E. (2008). Evaluation of marine alga Ulva lactuca as a source of natural preservative ingredient. American- Eurasian J. Agric. Environ. Sci. 3(3):434-444.

Orduña, J. D. Robledoa, J. and and Dawesd, C. J. (2002). Studies on the Tropical Agarophyte Gracilaria cornea J. Agardh (Rhodophyta, Gracilariales) from Yucatán, Mexico. I. Seasonal Physiological and Biochemical Responses. Botanica Marina Vol. 45, pp. 453–458.

Choi, J. S., Ha, Y., Lee, B., Moon, H. E., Cho, K. K. and Choi, I. S. (2014). Seasonal variation of antibacterial activities in the green alga Ulva pertusa kjellman. Journal of Environmental Biology. Vol., 35, 341 – 344.

Siddhanta, A. K., Goswami, A. M., Ramavat, B. K., Mody, K. H. and Mairh, O. P. (2001). Water soluble polysaccharides of marine algal species of Ulva (Ulvales, chlorophyta) pf indian water. Journal of Marine Science. Vol., 30, pp. 166 – 172.

Banerjee, K., Ghosh, R., Homechaudhuri, S. and Mitra, A. (2009). Seasonal variation in the biochemical composition of red seaweed (Catenella repens) from Gangetic delta, northeast coast of India. J. Earth Syst. Sci. 118, No. 5, pp. 497–505.

Arunkumar, K. and Sivakumar, S. R. (2012). Seasonal influence on bioactivity of seaweeds against plant pathogenic bacteria Xanthomonas axonopodis pv. citri (Hasse) Vauterin et al. African Journal of Microbiology Research. Vol. 6(20), pp. 4324-4331.

Plaza, M., Santoyo, S., Jaime, L., Garcia-Blairsy Reina, G., Herrero, M., Se˜norans, F. J. and Iba˜nez, E. (2010).Screening for bioactive compounds from algae. Journal of Pharmaceutical and Biomedical Analysis, 51, 450–455.

El Baroty, G. S., El-Baz, F. K., Abd Elmoein, I., Abd El Baky, H. H., Ali, M. M. and Ibrahim, E.A. ( 2011). Evaluation of the glycolipids of some Egyptian marine algae as a source of bioactive substances. International Research Journal of Pharmacay 2 (3), 165-174.

Sahayaraj, K., Asha, A. C., Rajesh, S. M. and Martin Rathi, J. A. (2014). Qualitative and quantitative profiles of secondary metabolites of chosen Chlorophyta and Ochrophyta from Gulf of Mannor. Cah. Biol. Mar, 55 : 69-76.

Ortiz , J., Romero, N., Robert, P., Araya, J. , Lopez-Herna´ndez, J., Bozzo, C., Navarrete, E., Osorio , A. and Rios, A. (2006). Dietary fiber, amino acid, fatty acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea Antarctica. Food Chemistry, 99, 98–104.

Yaich, H., Garna, H., Besbes, S., Paquot, M., Blecker, C. and Attia, H. (2011). Chemical composition and functional properties of Ulva lactuca seaweed collected in Tunisia Food Chemistry, 128, 895–901.

Khairy, H. M., El-Shafay, S. M. (2013). Seasonal variations in the biochemical composition of some common seaweed species from the coast of Abu Qir Bay, Alexandria, Egypt. OCEANOLOGIA, 55 (2).

Sun, Y., Zhan, Y. C., Sha, Y. and Pei, Y. H. (2007). Norisoprenoids from Ulva lactuca. J Asian Nat Prod Res 9: 321-325.

Liu, M., Hansen, P. and Lin, X., (2011). Bromophenols in Marine Algae and Their Bioactivities. Mar. Drugs 9, 1273-1292.

Silva, M., Vieira, L., Almeida, A. P. and Kijjoa, A. (2013). The Marine Macroalgae of the Genus Ulva: Chemistry, Biological Activities and Potential Applications. Oceanography 1: 101.


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