Bioconversion of Banana Pseudostem Fiber to Ethanol: Optimization of Acid Pretreatment Conditions and Fermentation Yeast Selection

Authors

  • Chien-Yih Lin
  • Meng-Tzu Peng
  • Yi-Chen Tsai
  • Shwu-Jene Tsai
  • Tsung-Yen Wu
  • Shiuan-Yuh Chien
  • Henry J Tsai Asia University, Taiwan

Keywords:

Banana Pseudostem, Bioethanol, Pretreatment, Fermentation.

Abstract

The banana (Musa sapientum) pseudostem is a massive agricultural leftover in Taiwan.  Conversion of such agricultural waste into cellulosic alcohol is an appealing idea and the aim of this study. This study optimized conditions for hydrolyzing banana  pseudostem with sulfuric acid during the pretreatment process and selected a best strain of yeast (Saccharomyces cerevisiae) for fiber hydrolysate fermentation.  Hydrolysis catalyzed by sulfuric acid reached a plateau at 5% concentration when measuring reducing sugar yield.  Five % sulfuric acid also demonstrated slightly higher sugar to alcohol conversion rate than 4% sulfuric acid during the subsequent fermentation.  The optimal fiber to acid ratio is 3:10 and 30-60 min boiling is sufficient.  Under 3:10 fiber/acid ratio with 4% sulfuric acid and 1 hr boiling, YA yeast can achieve 46% sugar to alcohol conversion rate.

 

References

J. Hansen, L. Nazarenko, R. Ruedy, M. Sato, J. Willis, A. Del Genio, D. Koch, A. Lacis, K. Lo, S. Menon, T. Novakov, J. Perlwitz, G. Russell, G.A. Schmidt, N. Tausnev, Earth's energy imbalance: confirmation and implications. Science 308, 1431-1435, 2005.

M. Scheffer, V. Brovkin, P.M. Cox, Positive feedback between global warming and atmospheric CO2 concentration inferred from past climate change. Geophys Res Lett 33, L10702, 2006.

Kyoto Protocol, Status of Ratification, United Nations Framework Convention on Climate Change, 2009.

A.J. Ragauskas, C.K. Williams, B.H. Davison, G. Britovsek, J. Cairney, C.A. Eckert, W.J. Frederick Jr, J.P. Hallett, D.J. Leak, C.L. Liotta, J.R. Mielenz, R. Murphy, R. Templer, T. Tschaplinski, The path forward for biofuels and biomaterials. Science 311, 484-489, 2006.

B.A. Simmons, D. Loque, H.W. Blanch, Next-generation biomass feedstocks for biofuel production. Genome Biol 9, 242, 2008.

F. Talebnia, D. Karakashev, I. Angelidaki, Production of bioethanol from wheat straw: An overview on pretreatment, hydrolysis and fermentation. Bioresour Technol 101, 4744-4753, 2010.

L. da Costa Sousa, S.P. Chundawat, V. Balan, B.E. Dale, 'Cradle-to-grave' assessment of existing lignocellulose pretreatment technologies. Curr Opin Biotechnol 20, 339-347, 2009.

G.W. Huber, B.E. Dale, Grassonline at the pump. Sci Am Jul, 52-59, 2009.

S.T. Li, Agricultural extension challenged by ECFA (in Mandarin). Taiw Agr Ext Anth 54, 307-312, 2009.

S. Chittibabu, M.K. Saseetharan, K. Rajendran, M. Santhanamuthu, Optimization of alkali pretreatment and enzymatic hydrolysis of Banana pseudostem for ethanol production by RSM. IEEE-International Conference on Advances in Engineering, Science and Management, 90-94, 2012.

H.S. Oberoi, P..V. Vadlani, L. Saida, S. Bansal, J.D. Hughes, Ethanol production from banana peels using statistically optimized simultaneous saccharification and fermentation process. Waste Manag 31, 1576-1584, 2011.

J.M.L. Reis, Fracture and flexural characterization of natural fiber-reinforced polymer concrete. Constr Build Mater 20, 673-678, 2006.

L.A. Pothan, S. Thomas, Polarity parameters and dynamic mechanical behaviour of chemically modified banana fiber reinforced polyester composites. Compos Sci Technol 63, 1231-1240, 2003.

N. Cordeiro, M.N. Belgacem, I.C. Torres, J.C.V.P. Mourad, Chemical composition and pulping of banana pseudo-stems. Ind Crops Prod 19, 147-154, 2004.

P. Kahar, K. Taku, S. Tanaka, Enzymatic digestion of corncobs pretreated with low strength of sulfuric acid for bioethanol production. J Biosci Bioeng 110, 453-458, 2010.

J. Zhao, L. Xia, Bioconversion of corn stover hydrolysate to ethanol by a recombinant yeast strain. Fuel Process Technol 91, 1807-1811, 2010.

J. Gabhane, S.P. William, A. Gadhe, R. Rath, A.N. Vaidya, S. Wate, Pretreatment of banana agricultural waste for bio-ethanol production: individual and interactive effects of acid and alkali pretreatments with autoclaving, microwave heating and ultrasonication. Waste Manag 34, 498-503, 2014.

I. De Bari, D. Cuna, V. Di Matteo, F. Liuzzi, Bioethanol production from steam-pretreated corn stover through an isomerase mediated process. N Biotechnol. 31, 185-195, 2014.

B. Bals, C. Rogers, M. Jin, V. Balan, B. Dale, Evaluation of ammonia fiber expansion (AFEX) pretreatment for enzymatic hydrolysis of switchgrass harvested in different seasons and locations. Biotechnol Biofuels 3, 1, 2010.

X. Meng, A.J. Ragauskas, Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates. Curr Opin Biotechnol 27C, 150-158, 2014.

G.L. Miller, Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31, 426-428, 1959.

H.J. Tsai, Function of interdomain alpha-helix in human brain hexokinase: covalent linkage and catalytic regulation between N- and C-terminal halves. J Biomed Sci 14, 195-202, 2007.

N. Spano, M. Ciulu, I. Floris, A. Panzanelli, M.I. Pilo, P.C. Piu, S. Salis, G. Sanna, A direct RP-HPLC method for the determination of furanic aldehydes and acids in honey. Talanta 78, 310-314, 2009.

P. Kahar, K. Taku, S. Tanaka, Enzymatic digestion of corncobs pretreatment with low strength of sulfuric acid for bioethanol production. J Biosci Bioeng 110, 453-458, 2010.

B.C. Saha, L.B. Iten, M.A. Cotta, Y.V. Wu, Diluted acid pretreatment, enzymatic saccarification and fermentation of wheat straw to ethanol. Process Biochem 40, 3693-3700, 2005.

L. Canilha, V.T. Santos, G.J. Rocha, J.B. Almeida e Silva, M. Giulietti, S.S. Silva, M.G. Felipe, A. Ferraz, A.M. Milagres, W. Carvalho, A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid. J Ind Microbiol Biotechnol 38, 1467-1475, 2011.

R.C. Kuhad, R. Gupta, Y.P. Khasa, A. Singh, Bioethanol production from Lantana camara (red sage): Pretreatment, saccharification and fermentation. Bioresour Technol 101, 8348-8354, 2010.

C. Martin, B. Alriksson, A. Sjöde, N.O. Nilvebrant, L.J. Jönsson, Dilute sulfuric acid pretreatment of agricultural and agro-industrial residues for ethanol production. Appl Biochem Biotechnol 137-140, 339-352, 2007.

E. Palmqvist, B. Hahn-Hagerdal, Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour Technol 74, 25-33, 2000.

Downloads

Published

2015-08-14

How to Cite

Lin, C.-Y., Peng, M.-T., Tsai, Y.-C., Tsai, S.-J., Wu, T.-Y., Chien, S.-Y., & Tsai, H. J. (2015). Bioconversion of Banana Pseudostem Fiber to Ethanol: Optimization of Acid Pretreatment Conditions and Fermentation Yeast Selection. Asian Journal of Agriculture and Food Sciences, 3(4). Retrieved from https://www.ajouronline.com/index.php/AJAFS/article/view/2763

Issue

Section

Articles