Bioconversion of Banana Pseudostem Fiber to Ethanol: Optimization of Acid Pretreatment Conditions and Fermentation Yeast Selection
Keywords:Banana Pseudostem, Bioethanol, Pretreatment, Fermentation.
The banana (Musa sapientum) pseudostem is a massive agricultural leftover in Taiwan.Â Conversion of such agricultural waste into cellulosic alcohol is an appealing idea and the aim of this study. This study optimized conditions for hydrolyzing banana Â pseudostem with sulfuric acid during the pretreatment process and selected a best strain of yeast (Saccharomyces cerevisiae) for fiber hydrolysate fermentation. Â Hydrolysis catalyzed by sulfuric acid reached a plateau at 5% concentration when measuring reducing sugar yield. Â Five % sulfuric acid also demonstrated slightly higher sugar to alcohol conversion rate than 4% sulfuric acid during the subsequent fermentation.Â The optimal fiber to acid ratio is 3:10 and 30-60 min boiling is sufficient.Â Under 3:10 fiber/acid ratio with 4% sulfuric acid and 1 hr boiling, YA yeast can achieve 46% sugar to alcohol conversion rate.
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