Mycoflora of Wheat (<i>Triticum aestivum L.</i>) at Different Locations in Hail Area- Saudi Arabia

Authors

  • Elham Sharief Dawood Nile Valley University
  • Modhi K. Elshamry

Abstract

600 grain samples collected from wheat fields in Hail area at the northern part of Saudi Arabia was used for this study. Isolation and identification of seed-borne fungi were conducted according to standard tests described by the International Seed Testing Association (ISTA) using YGCA medium. A total of 505 of external mycoflora and 705 of internal mycoflora  were grouped into five fungal genera namely, Aspergillus ; Alternaria  ; Penillium ; Fusarium and Ulocladium spp. were isolated. Comparsion  between  frequencies and relative densities of external and internal  mycoflora was carried out among the species of the predominant genera. Aspergillus flavus and A. niger reaveled high Fr. and RD of external mycoflora (A. flavus Fr.67.5 - 48.2% , RD. 44.3 - 38.3% and  A. niger Fr. 50.8 - 50.8% and RD. 41.4-36.4 respectively). All the species of Ulocladium and Alternaria were predominant as internal mycoflora except Alternaria  alternata . The most predominant species of Ulocladium and Alternaria were U. atrium (Fr 84.2% -75.8 and RD 88.4%-76.6 as internal – external mycoflora respectively)  and Alternaria alternate (Fr 75.5% - 45.2% and RD. 37.2 - 68.8  as external –internal mycoflora respectively).

 

Author Biography

Elham Sharief Dawood, Nile Valley University

Associate professor of microbial biotechnology at department of life science faculty of education nile valley university

References

A. Hussain, A. Saboor, M.A. Khan, A.Q. Mohsin and F. Hassan. Technical efficiency of wheat production in rain-fed areas: a case study of Punjab, Pakistan. Pak. J. Agri. Sci. vol., 49 no. 3, pp. 411- 417, 2012

Anonymous,.Agricultural Statistics of Saudi Arabia. Ministry of Agriculture, 2004 .

I. Niaz. and S. Dawar. Detection of seed borne mycoflora in maize (Zea mays L.). Pak. J. Bot.vol. 41no.1, pp. 443- 45, 2009.

M.V.Wiese. Compendium of wheat diseases (3rd Ed). St. Paul, USA: APS Press, 112 pp. 1998.

M.B. Ilyas, S.A.A. Bokhari, and M.A. Khan. Fungi detected from wheat seeds exhibiting black points symptoms and their control by seed treatment. Pak. J. Phytopathol., vol.10, pp. 86-89, 1998.

A. Rehman, K. Sultana, N. Minhas, M. Gulfraz, G.K. Raja, and Z. Anwar. Study of most prevalent wheat seed-borne mycoflora and its effect on seed nutritional value. Afr. J. Microb. Res.vol. 5 no.25 pp. 4328- 4337, 2011.

SB Mathur, O. Kongsdal .Common Laboratory Seed Health Testing Methods for Detecting Fungi. International Seed Testing Association, Bassersdorf, Switzerland, p. 427 2003

LA Castlebury, DF .Farr .The Genus Tilletia in the United States, Systematic Mycology and Microbiology Laboratory, ARS, USDA., 2011,

R. R. Nelson, and F. A. Haasis, .The perfect Stage of Curvularia lunata. Mycologia. Vol.56 pp. 316- 317, 1964.

J.I. Pitt and A.D.Hocking. Fungi and food spoilage. Chapman and Hall, New York, USA. 593 p, 1997 .

HH .Gonzalez, GA. Molto, A .Pacin, SL. Resnik, MJ .Zelayaand M. Masana. Trichothecenes and mycoflora in wheat harvested in nine locations in Buenos Aires province, Arg. Myco.vol. 165(2), pp. 105-14, 2008.

JL Devore. Probability and Statistics for Engineering and the Sciences.Monterrey, California. Brooks-Cole Publishing Company,vol. 1987, pp. 352-354, 2008.

Statistix Version 1.0.User's Manual. Analytical Software. Borland International Inc. and Fleming Software, 1996.

G. Barros, A. Torres, and S. Chulze. Aspergillus flavus population isolated from soil of Argentina’s peanut-growing region. Sclerotia production and toxigenic profile. J.of Food Sci. and Agr. Vol. 85, pp. 2349-2353, 2005.

M. Bashir, M. A Mani, and, A.S Kutama. Seed-Borne mycoflora of local and improved wheat (Triticum aesativum L.). cultivars in Kano, Nigeria . Bayero J of Pure and App. Sci., vol. 5 no, 2, 101 – 103, 2012.

A.S. Mohammed, and A.S. Kutama. Isolation and identification of fungal mycoflora associated with groundnut (Arachishypogea L.) in different storage facilities. Bioand Env. Sciences J. Trop. vol.41 pp.131-134, 2007.

BW. Horn. Colonization of wounded peanut by soil fungi: selectivity for species from Aspergillus section flavi. Myco. Vol. 23, pp. 425-430, 2005.

A. Habib, ST. Sahi, Javed and N. Ahmad. Prevalence of seed-borne fungi on wheat during storage and its impact on seed germination. Pak. J. Phyt.vol. 23 no.1, 42- 47, 2011.

U.L. Diener, R.J. Cole, RA. Hill. Ann. Rev. Phyto. vol.25, pp 249, 1987 .

. SI .Abdel-Hafez, AH. Moubasher, Shoreit, AA .Ismail. Fungal flo¬ra associated with combine harvester wheat and sorghum dusts from Egypt. J. Basic Microbiol. Vol. 30 no.7, pp. 467-79, 1990

M.L. Abraca,. Bragulat, F.J. Cabanes. App.Env.Micr. vol.60, pp.2650, 1994.

Downloads

Published

2015-06-15

How to Cite

Dawood, E. S., & Elshamry, M. K. (2015). Mycoflora of Wheat (<i>Triticum aestivum L.</i>) at Different Locations in Hail Area- Saudi Arabia. Asian Journal of Agriculture and Food Sciences, 3(3). Retrieved from https://www.ajouronline.com/index.php/AJAFS/article/view/2617

Issue

Vol. 3 No. 3 (2015): June 2015

Section

Articles

License

  • Papers must be submitted on the understanding that they have not been published elsewhere (except in the form of an abstract or as part of a published lecture, review, or thesis) and are not currently under consideration by another journal published by any other publisher.
  • It is also the authors responsibility to ensure that the articles emanating from a particular source are submitted with the necessary approval.
  • The authors warrant that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required.
  • The authors ensure that all the references carefully and they are accurate in the text as well as in the list of references (and vice versa).
  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
  • The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.