Occurrence of <i>Alfalfa Mosaic Virus </i>(AMV) Infecting Bean Crop in Burdur Province, Turkey


  • H. Çulal Kiliç Süleyman Demirel University, Agriculture Faculty, Plant Protection Department Isparta, Turkey
  • Nejla Yardimci Süleyman Demirel University, Agriculture Faculty, Plant Protection Department Isparta, Turkey


Virus, detection, ELISA, IC-RT-PCR


During surveys conducted in 2012- 2013 growing season, field-grown bean plants with symptoms of mosaic, chlorotic mottling, vein banding, blistering and leaf malformation were observed. A total of 118 leaf samples were collected from various locations in this region. Alfalfa mosaic virus (AMV), was detected in bean, using enzyme-linked immunosorbent assay (ELISA), and immunocapture-reverse-transcription-polymerase chain reaction (IC-RT-PCR) methods. ELISA tests showed that among 118 field samples tested, 7 were infected with AMV. Seven leaf samples that had tested positive in DAS-ELISA were used for IC-RT-PCR. IC-RT-PCR was carried out by using specific primer which amplified a 351 bp fragment of coat protein of AMV in samples. The presence of AMV in the leaf samples was further confirmed IC-RT-PCR using specific primers.


Açıkgöz S. The Identification of viruses on Phaseolus vulgaris L. and their distribution and damages in Erzincan and Erzurum Regions. PhD diss., Agric. Facu., Univ. Atatürk, Erzurum, 1984.

Choi HS, Kim MK, Park JW, Lee SH, Kim KH, Kim JS, Were HK, Choi JK, Takanami Y. First report of the Peanut stripe strain of Bean common mosaic virus (BCMV-PSt) Infecting Mungbean in Korea. Plant Pathology Journal, 22(1), 46-50, 2006.

Choi SK, Choi JK, Park WM, Ryu KH. RT-PCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. Journal of Virological Methods, 83, 67-73, 1999

Clark MF, Adams AN. Characteristic of microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475-483, 1977

Çalışkan S. Current situation, problems and solutions of bean in TR71 region. Turkish Journal of Agriculture- Food Science Technology, 2(2), 60-65, 2014.

Dietzgen RG. Application of PCR in Plant Virology. In: Khan A, Dijkstra J, editors. Plant Viruses as Molecular Pathogens. Chapter 21 New York: JHaworth Press. Inc, 2001.

El-Aal HA, El-Hwat N, El-Hefnawy N, Medany M. Effect of sowing dates, irrigation levels and climate change on yield of common bean (Phaseolus vulgaris L.). American Eurasian Journal of Agricultural and Environment Sciences, 11(1), 79-86, 2011.

Ghorbani SGM, Shahraeena N, Elahinia SA. Distribution and impact of virus associated diseases of common bean (Phaseolus vulgaris L.) in northern Iran. Arch. Phyto. Plant Protection, 43(12), 1183–1189, 2010.

Güzel Ö, Arlı-Sökmen M. Determination of some viruses infecting common bean (Phaseoulus vulgaris L.) and their incidences in seed lots in Samsun Province. The Journal of Turkish Phytopathology, 32( 2): 99-106, 2003

Hassan M, Myrta A. Polak J. Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR. Journal of Virological Methods,133, 124-129. 2006.

Herranz MC, Sanchez-Navarro JA, Sauri A, Mingarro I, Pallas V. Mutational analysis of the RNA-binding domain of the Prunus necrotic ringspot virus (PNRSV) movement protein revealsits requirement for cell-to-cell movement. Virology, 339, 31–41. 2005.

Jaspars EMJ, Bos L. Alfalfa mosaic virus. CMI/AAB Descriptions of Plant viruses. No: 229. 1980

Kaiser WJ, Hannan RM. Additional hosts of alfalfa mosaic virus and its seed transmission in tumble pigweed and bean. Plant Disease Reptr, 59, 770, 1983

Kutluk-Yılmaz ND, Gümüş M, Erkan S. Studies on determination of virus diseases in the seeds of bean from Tokat Province. J. Aegean Univesity, Agriculture Faculty, 39(3), 49-55, 2002

Loebenstein G, Thottappilly G. Virus and virus-like diseases of major crops in developing countries. 840p., Kluwer Academic Publishers: Dordrecht, 2004.

McLaughlin MR. A greenhouse method for aphid inoculation of alfalfa mosaic virus in white clover by co-culture of virus, vector and clover. P. 318. In: Peters DC, Webster JA, Chloubers CS, editors. Aphid plant interaction: populations to molecules. Okla. Agric. Exp. Stn. Stillwater OK, 1991.

Petrovic D, Ignjatov M, Nikolic Z, Vujakovic M, Vasin M, Milosevic M, Taski-Adjukovic, K. Occurrence and distribution of viruses infecting the bean in Serbia. Archives of Biological Science, 62(3), 595-601, 2010

Rowhani A, Manningas MA, Lile LS, Daubert SD, Golino DA. Development of a detection system for viruses of woody plants based on PCR analysis of immobilized virions. Phytopathology, 85, 347–352, 1995.

Saade M, Aparicio F, Sanchez-Navarro JA, Herranz MC, Myrta A, Terlizzi BD, Pallas V. Simultaneous detection of the three ilarviruses affecting stone fruit trees by nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction. Phytopathology, 90(12), 1330-1336, 2000.

Sanchez-Navarro JA, Herranz MC, Pallás V. Cell-to-cell movement of alfalfa-mosaic virus can be mediated by the movement proteins of ilar-, bromo-, cucumo-, tobamo- and comoviruses and does not require virion formation.Virology, 346, 66-73, 2006.

Sanchez-Navarro JA, Aparicio F, Rowhani A, Pallas V. Comparative analysis of ELISA, nonradioactive molecular hybridization and PCR for the detection of Prunus necrotic ringspot virus in herbaceous and prunus hosts. Plant Pathology, 47, 780-786, 1998

Singh SP, Gepts P, Debouck DG.. Races of common bean (Phaseolus vulgaris, Fabaceae). Ecology Botany, 45:379–396, 1991.

Webster CG, Wylie SJ, Jones MGK. Diagnosis of plant viral pathogens. Current Science, 86, 12, 2004

Xu H, Nie J. Identification, characterization, and molecular detection of Alfalfa mosaic virus in Potato. Virology, 96,11, 2006

Zitikaite I, Samuitiene M. Identification and some properties of Alfalfa mosaic alfamovirus isolated from naturally infected tomato crop. Biologija, 54(2), 83-88, 2008




How to Cite

Çulal Kiliç, H., & Yardimci, N. (2015). Occurrence of <i>Alfalfa Mosaic Virus </i>(AMV) Infecting Bean Crop in Burdur Province, Turkey. Asian Journal of Agriculture and Food Sciences, 3(2). Retrieved from https://www.ajouronline.com/index.php/AJAFS/article/view/2520