Recent Advance on the Methods for Rapid Detection of Foodborne Pathogens


  • Shijun Fu
  • Shijin Guo
  • Sanyang Gao
  • Zhiqiang Shen


Rapid detection and identification of foodborne pathogens is an important prerequisite for timely and effective control and prevention of pathogen spread and food poisoning. Culture-based methods are too time-consuming for high-throughput testing and too tedious for analysis of samples with multiple organisms and provide little clinical information regarding the pathogen. With continued development of novel molecular-based technologies for rapid, high-throughput detection of foodborne pathogenic bacteria, a number of unique approaches and variations on existing techniques are currently on the market or are being implemented that offer ease of use, reliability and low cost. In this paper, the most frequently used assays for foodborne pathogens, including immunomagnetic separation, DNA-hybridization, capillary zone electromigration, conventional PCR, multiplex PCR, real-time PCR, DNA microarray, LAMP and biosensors were reviewed.



Alarcón B, García-Cañas V, “Simultaneous and sensitive detection of three food-borne pathogens by multiplex PCR, capillary gel electrophoresis and laser induced fluorescenceâ€, Journal of Agricultural and Food Chemistry, vol. 52, pp. 7180-7186, 2004.

Al-Khaldi SF, Martin SA, “DNA microarray technology used for studying foodborne pathogens and microbial habitats: minireviewâ€, Journal of AOAC International, vol. 85, no.4 , pp. 906-910, 2002.

Castro-Puyana M, García-Cañas V, “Recent advances in the application of capillary electromigration methods for food analysis and Foodomicsâ€, Electrophoresis, vol. 33, no.1, pp. 147-167, 2012.

Chen S, Wang F, “Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplificationâ€, Applied Environmental Microbiology, vol. 77, no. 12, pp. 4008-4016, 2011.

do Nascimento C, Ferreira de Albuquerque Junior R, “Use of the DNA Checkerboard hybridization method for detection and quantitation of Candida species in oral microbiotaâ€, Canadian Journal of Microbiology, vol. 55, no. 5, 622-626, 2009.

Donhauser SC, Niessner R, “Sensitive quantification of Escherichia coli O157:H7, Salmonella enterica, and Campylobacter jejuni by combining stopped polymerase chain reaction with chemiluminescence flow-through DNA microarray analysisâ€, Analytical Chemistry, vol. 83, no.8, pp. 3153-3160, 2011.

Fu Z, Rogelj S, “Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR. International Journal of Food Microbiology, vol. 99, no.1, pp. 47-57, 2005.

García-Cañas V, Cifuentes A, “Recent advances in the application of capillary electromigration methods for food analysis and Foodomicsâ€, Electrophoresis, vol. 35, no. 1, pp. 147-169, 2014.

Gardner SN, Jaing CJ, “A microbial detection array (MDA) for viral and bacterial detectionâ€, BMC Genomics, vol. 11, pp. 668, 2010.

Gordillo R, Córdoba JJ, “Development of PCR assays for detection of Escherichia coli O157:H7 in meat productsâ€, Meat Science, vol. 88, no. 4, pp. 767-773, 2011.

Gracias KS, McKillip JL, “A review of conventional detection and enumeration methods for pathogenic bacteria in foodâ€, Canadian Journal of Microbiology, vol. 50, no. , pp.883-890, 2004.

Hara-Kudo Y, Yoshino M, “Loop-mediated isothermal amplification for the rapid detection of Salmonellaâ€, FEMS Microbiology Letters, vol. 253, no. 1, pp. 155-161, 2005.

Herrero M, García-Cañas V, “Recent advances in the application of capillary electromigration methods for food analysis and foodomicsâ€, Electrophoresis, vol. 31, no. 1, pp. 205-228, 2010.

Hill WE, “The polymerase chain reaction: applications for the detection of foodborne pathogensâ€, Critical Reviews in Food Science and Nutrition, vol. 36, no. 1-2, pp. 123-173, 1996.

Kim JS, Lee GG, “A novel multiplex PCR assay for rapid and simultaneous detection of five pathogenic bacteria: Escherichia coli O157:H7, Salmonella, Staphylococcus aureus, Listeria monocytogenes, and Vibrio parahaemolyticusâ€, Journal of Food Protection, vol. 70, no. 7, pp. 1656-1662, 2007.

Kostrzynska M, Bachand A, “Application of DNA microarray technology for detection, identification, and characterization of foodborne pathogensâ€, Canadian Journal of Microbiology, vol. 52, no. , pp. 1-8, 2006.

Lee SH, Jung BY, “A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meatsâ€, Journal of Veterinary Science, vol. 10, no. 1, pp. 43-51, 2009.

Miller ND, Davidson PM, “Real-time reverse-transcriptase PCR for Salmonella Typhimurium detection from lettuce and tomatoesâ€, LWT-Food Science and Technology, vol. 44, pp. 1088-1097, 2011.

Naravaneni R, Kaiser J, “Rapid detection of foodborne pathogens by using molecular techniquesâ€, International Journal of Medical Microbiology, vol. 54, pp. 51-54, 2005.

O’Grady J, Sedano-Balbás S, “Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic targetâ€, Food Microbiology, vol. 25, no. 1, pp. 75-84, 2008.

Olsen KN, Brockmann E, “Quantification of Leuconostoc populations in mixed dairy starter cultures using fluorescence in situ hybridizationâ€, Journal of Applied Microbiology, vol. 103, no. 4, pp. 855-863, 2007.

Park SH, Hanning I, “Multiplex PCR assay for the detection and quantification of Campylobacter spp., Escherichia coli O157:H7, and Salmonella serotypes in water samplesâ€, FEMS Microbiology Letter, vol. 316, no. 1, pp. 7-15, 2011.

Rabanes HR, Guidote JrM, “Capillary electrophoresis of natural products: Highlights of the last five years (2006-2010)â€, Electrophoresis, vol. 33, no. 1, pp. 180-195, 2012.

Rasooly A, Herold KE, “Food microbial pathogen detection and analysis using DNA microarray technologiesâ€, Foodborne Pathogens Dis, vol. 25, no. 4, pp. 531-550, 2008.

Ravindranath SP, Wang YL, “SERS driven cross-platform based multiplex pathogen detectionâ€, Sensors and Actuators B, vol. 152, no. , pp. 183-190, 2011.

Rijpens NP, Herman LM, “Molecular methods for identification and detection of bacterial food pathogensâ€, Journal of AOAC International, vol. 85, pp. 984-995, 2002.

Rijpens NP, Herman LM, “Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCRâ€, International Journal of Food Microbiology, vol. 46, no. 1, pp. 37-44, 1999.

Riyaz-Ul-Hassan S, Verma V, “Rapid detection of Salmonella by polymerase chain reactionâ€, Molecular and Cellular Probes, vol. 18, no. 5, pp. 333-339, 2004.

Salam F, Tothill IE, “Detection of Salmonella typhimurium using an electrochemical immunosensorâ€, Biosen Bioelectron vol. 24, no. 8, pp. 2630-2636, 2009.

Sergeev N, Herold KE, “Microarray analysis of microbial pathogens. In: Encyclopedia of Rapid Microbiological Methodsâ€, Volume 3, DHI Publishing, River Grove, IL, 2006.

Skjerve E, Olsvik Ø, “Immunomagnetic separation of Salmonella from foodsâ€, International Journal of Food Microbiology, vol. 14, no. 1, pp. 11-17, 1991.

Sockett PN, “The economic implications of human Salmonella infectionâ€, Journal of Applied Bacteriology, vol.71, no. 4, pp. 289-295, 1991.

Spano G, Beneduce L, “Real-time PCR for the detection of Escherichia coli O157:H7 in dairy and cattle wastewaterâ€, Letters in Applied Microbiology, vol. 40, no. 3, pp. 164-171, 2005.

Su HC, Ma Q, “Gold nanoparticles as colorimetric sensor: A case study on E. coli O157:H7 as a model for Gram-negative bacteriaâ€, Sensors and Actuators B, vol. 161, pp. 298-303, 2012.

Techathuvanan C, Draughon FA, “Loop-mediated isothermal amplification (LAMP) for the rapid and sensitive detection of Salmonella Typhimurium from porkâ€, Journal of Food Science, vol. 75, no. 3, pp. 165-172, 2010.

Touron A, Berthe T, “Detection of Salmonella in environmental water and sediment by a nested-multiplex polymerase chain reaction assayâ€, Research in Microbiology, vol. 156, no. 4, pp. 541-553, 2005.

Trevanich S, Tiyapongpattana S, “Application of an optimized 18-h method involving one step culturing and single primer-based PCR assay for detection of Salmonella spp. in foodsâ€, Food Control , vol. 21, no. 5, pp. 593-598, 2010.

Villamizar RA, Maroto A, “Fast detection of Salmonella infants with carbon nanotube field effect transistorsâ€, Biosensors and Bioelectronics, vol. 24, no. 2, pp. 279-283, 2008.

Wang H, Li Y, “Rapid, sensitive, and simultaneous detection of three foodborne pathogens using magnetic nanobead-based immunoseparation and quantum dot-based multiplex immunoassayâ€, Journal of Food Protection, vol. 74, no. 12, pp. 2039-2047, 2011.

Wang M, Cao B, “Detection of Enterobacter sakazakii and other pathogens associated with infant formula powder by use of a DNA microarrayâ€, Journal of Clinical Microbiology, vol. 47, no. 10, pp. 3178-3184, 2009.

Wang XW, Zhang L, “Development and application of an oligonucleotide microarray for the detection of foodborne bacterial pathogensâ€, Applied Microbiology and Biotechnology, vol. 76, no. 1, pp. 225-233, 2007.




How to Cite

Fu, S., Guo, S., Gao, S., & Shen, Z. (2014). Recent Advance on the Methods for Rapid Detection of Foodborne Pathogens. Asian Journal of Agriculture and Food Sciences, 2(5). Retrieved from