Antifungal Study of <i>Bacillus Subtilis</i> BR2 on <i>Ganoderma</i> sp., The Cause of Basal Stem Rot Disease in Oil Palm Plants

Authors

  • Dr. Rustam Riau Assessment Institute of Agricultural Technology

Keywords:

Keywords, Bacillus subtilis, palm oil, stem rot, Ganoderma sp.

Abstract

Bacillus subtilis BR2 is antagonistic bacteria has the ability to inhibit the growth of several fungal pathogens such as Fusarium oxysporum, Helminthosporium maydis, Pyricularia oryzae, and Rhizoctonia solani, respectively as cause wilt in watermelon plants, leaf spot in corn plants, blast in rice, and leaf blight of rice plants. The research aims to determine the ability of  B. subtilis BR2 suppress the growth of fungi Ganoderma sp. causing basal stem rot of oil palms. The study was conducted at the level of  in vitro, by testing inhibition of  B. subtilis BR2 on solid and liquid medium. The results showed that isolate of B. subtilis BR2 able to suppress the growth of fungi Ganoderma sp., with percentage of 80% inhibition in a solid medium and 78-97% in a liquid medium. Thus, isolates of  B. subtilis BR2 has antibiosis mechanism in addition to mechanisms other antagonist to inhibit the growth of fungi Ganoderma sp.

 

Author Biography

Dr. Rustam, Riau Assessment Institute of Agricultural Technology

Agriculture

References

Statistics Indonesia. 2015. Plantation area by Province and Crops, Indonesia (000 Ha), 2012-2014*. http://www.bps.go.id/linkTableDinamis/ view/id/838. (30 September 2015).

Agrios GN. 2005. Plant Pathology. Ed ke-5. San Diego: Academic Press.

Darmono TW. 1998. Development and survival of Ganoderma sp. In oil palm tissue. International Oil Palm Conference. Bali, Indonesia: Indonesian Oil Palm Research Institute.

Susanto A. 2011. Ganoderma in oil palm plantations from time to time. National Symposium and Workshop Ganoderma: As Pathogen Raw Material Plant Diseases and Traditional Medicine. Bogor, 2-3 November 2011.

Darmono TW. 1996. Biotechnological approaches to overcome the problems stem rot disease due to attacks by the Palm Oil Ganoderma.Warta Puslit Biotek Perkebunan 1, 17-25.

Naher L, Yusuf UK, Ismail A, Tan SG, Mondal MMA. 2013. Ecological status of Ganoderma and basal stem rot disease of oil palms (Elaeis guineensis Jacq.). AJCS 7 : 1723-1727.

Nur Ain Izzati MZ, Abdullah F. 2008. Disease suppression Ganoderma-infected oil palm seedlings treated with Trichoderma harzianum. Plant Protect. Sci. 44: 101-107.

Rustam. 2011. Potential Bacillus isolates that are chitinolytic as biological agents and the mass production of organic waste. In Proceedings of the National Seminar, Agricultural Technology Innovation Specific Location, Bogor, 19-20 November 2011.

Rustam, Giyanto, Suryo Wiyono, Dwi Andreas Santosa, Slamet Susanto. 2011. Selection and identification of antagonistic bacteria as biological control of rice sheath blight disease. Journal of Agricultural Research 30 (3): 164-171.

Rustam. 2012. Characterization and Identification of Bacteria as Biological Agents to Control the Rice Sheath Blight Disease. Prosiding International Seminar of Rice Technology Innovation for Increasing Production and Conserving Enviroment Under, Global Climate Change, Subang-Indonesia, July 11-12, 2012.

Szczech M, Shoda M. 2005. The influence of Bacillus subtilis RB14-C on the development of Rhizoctonia solani and indigenous microorganisms in the soil. Canadian Journal of Microbiology 51: 405-411.

Wibowo RH. 2011. Controlling attacks stem rot (Ganoderma boninense Pat.) On plant seeds of oil palm (Elaeis guineensis Jacq.) Using bacterial isolates chitinolytic. Thesis of University of North Sumatra.

Herliyana EN, Darmono TW, Minarsih H, Firmansyah MA, Dendang B. 2011. Control of Ganoderma sp attack. (60-80%) in plants sengon as coffee and cocoa plant protector. Journal of Agricultural Sciences Indonesia16: 14-27.

Rustam. 2013a. Anti-fungal efficacy of bioactive compounds from Bacillus subtilis BR2 on the growth of Rhizoctonia solani causing the rice sheath blight disease. In Proceedings of the National Seminar: Accelerating Innovation and Technology Dissemination Towards Independence and Food-Based Local Genetic Resources, Palu, March 18, 2013. Bogor: Center for Technology Assessment and Development of Agriculture.

Liu CH, Chen X, Liu TT, Lian B, Yucheng Go, Caer V, Xue YR, Wang BT. 2007. Study of the antifungal activity of Acinetobacter baumannii of its antifungal components. Appl Microbiol Biotechnol 76: 459-466.

Rustam. 2013b. Phytotoxicity and stability of the anti-fungal bioactive compounds of several isolates of biological agents against the cause of rice sheath blight disease. In Proceedings of the National Seminar: Accelerating Innovation and Technology Dissemination Towards Independence and Food-Based Local Genetic Resources, Palu, March 18, 2013. Bogor: Center for Technology Assessment and Development of Agriculture

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Published

2016-06-15

How to Cite

Rustam, D. (2016). Antifungal Study of <i>Bacillus Subtilis</i> BR2 on <i>Ganoderma</i> sp., The Cause of Basal Stem Rot Disease in Oil Palm Plants. Asian Journal of Engineering and Technology, 4(3). Retrieved from https://www.ajouronline.com/index.php/AJET/article/view/3542

Issue

Vol. 4 No. 3 (2016): June 2016

Section

Articles

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