A Potential Elicitor of Green Alga (Ulva lactuca) and Commercial Algae Products against Late Blight Disease of Solanum tuberosum L.
Keywords:
Algae, Potato, Late blight, ElicitorAbstract
The phenomenon of systemic acquired resistance has attracted attention as a new strategy for controlling plant disease. The resistance was evident as a reduction in disease incidence compared with infected control. The objective of this research was to determine the efficacy of green alga (Ulva lactuca) extract and some Commercial algae products for management of late blight disease of two varieties of potatoes (Lady pulford and Burren) under field conditions. Also, we investigated the ability of these algae to elicit induced resistance in potato against late blight disease caused by Phytophthora infestans, through, their effects on biochemical changes which lead to induction of plant defense against the pathogen. A negative correlation between the disease severity and the content of total soluble polyphenols , this correlation reached to 50% in Burren variety while it was 67% in the variety Lady pulford. Negative correlation between the disease severity index and total protein was found also and the correlation reached to 80% in the variety Lady pulford. The same trend was also noticed in PPO and RNase. So, Lady Pulford is more tolerant to the disease than Burren. Finally, we could say the use of algae extract might help to overcome the pathogen infection by increasing levels of defense-related enzymes and phenolic and protein substances.References
FAO, FAOSTAT, FAO Statistical Databases. http://faostat.fao.org. 2012.
FAO, FAOSTATdata, http://faostat.fao.org. 2014.
El-Sirafy, Z. M., Abbady, K. A., El-Ghamry, A. M. and El-Dissoky, R. A. Potato Yield Quality, Quantity and Profitabilty as Affected by Soil and Foliar Potassium Application.Research Journal of Agriculture and Biological Sciences. 4(6): 912-922. 2008.
El-Mougy, N. S. Effect of some essential oils for limiting early blight (alternariasolani) development in potato field.Journal of Plant Protection Research. 49, 1: 57-62. 2009.
Abd-EL-Khair, H. and Haggag, W.M. Application of some Egyptian medicinal plant extracts against potato late and early blights. Res. J. Agric. Biol. Sci., 3(3): 166–175. 2007.
Kurzawińska, H. and Mazur, S. The usefulness of chitosan and Pythium oligandrum in potato tuber protection against Helminthosporium solani. Folia Hortic., 20(2): 67–74. 2008.
Cwalina-Ambroziak, B. and Trojak, A. Effectiveness of selected fungicides in potato protection against Phytophthora infestans and Alternaria spp. Pol. J. Nat. Sc., 26(4): 275–284. 2011.
Soytong, K. and Ratanacherdchai, K. Application of myco fungicide to control late blight of potato.Journal of Agricultural Technology. 1 (1): 19-32. 2005.
Rahman, M. M., Dey, T. K., Ali, M. A., Khalequzzaman, K. M. and Hussain, M. A. Control of late blight disease of potato by using new fungicides. Int. J. Sustain. Crop Prod. 3(2):10-15. 2008.
Ahmed, S. M. Impact of foliar applied fungicides on late blight disease, yield and yield components of three varieties of potatoes. Journal of Applied Sciences Research. 6 (8): 994 – 1001. 2010.
Mohana, D. C., Prasad, P., Vijaykumar, V., and Raveesha, K. A. Plant extracts effect on Seed-borne pathogenic fungi from seeds of paddy grown in southern India. Journal of Plant Protectection Research, 51(2): 101-106. 2011.
Demirel, Z., Yilmaz-Koz, F., Karabay-Yavasoglu, U., Ozdemir, G., and Sukatar, A. Antimicrobial and antioxidant activity of brown algae from the Aegean Sea. Journal of Serbian Chemical Society, 74 (6): 619-628. 2009.
Jiménez, E., Dorta, F., Medina, C., RamÃrez, C., RamÃrez, I. and Cortés, H. Anti-Phytopathogenic Activities of Macro-Algae Extracts. Mar.Drug 9:739-756. 2011.
Dhargalkar, V.K. and Pereira, N. Seaweed: promising plant of the millennium. Science and Culture 71, 60–66. 2005.
Kong, F., Mao, Y., Cui, F., Zhang, X. and Gao, Z. Morphology and Molecular Identification of Ulva Forming Green Tides in Qingdao China. J Ocean University of China 10: 73-79. 2011.
Mandal, S., N. Mallick and A. Mitra, Salicylic acid- induced resistance to Fusarium oxysporumf. sp.lycopersici in tomato. Plant Physiology and Biochemistry, 47, 642-649. 2009.
Abd-El-Kareem, F. Induced Resistance in Bean Plants Against Root Rot and Alternaria Leaf Spot Diseases Using Biotic and Abiotic Inducers under Field Conditions. Research Journal of Agriculture and Biological Sciences, 3(6): 767-774. 2007.
Sarwar, N., Hayat-Zahid, C.H., Ikramul, H.A.Q. and Jamil, F.F. Induction of systemic resistance in chickpea against Fusarium wilt by seed treatment with Salicylic acid and bion. Pakistan Journal of Botany, 37(4):989-995. 2005.
Brimner, T.A. and Boland, G. J. A review of the non-target effect of fungi used to biologically control plant diseases. Agriculture, Ecosystem and environment. 100, 3-16. 2003.
Klarzynski, O., Plesse, B., Joubert, J.M., Yvin,J.C., Kopp,M., Kloareg, M. and Fritig,B. Linear b-1,3 Glucans Are Elicitors of Defense Responses in Tobacco. Plant Physiology,Vol. 124, pp. 1027–1037, 2000.
Mercier, L., Lafitte, C., Borderies, G., Briand, X., Esquerré-Tugayé, M. T. and Fournier, J. The algal polysaccharide carrageenans can act as an elicitor of plant defence. New Phytologist, 149, 43–51. 2001.
Aziz, A., Poinssot, B., Daire, X., Adrian, M., Bezier, A. and Lambert, B. Laminarin elicits defense responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola. Molecular Plant-Microbe Interactions, 16, 1118–1128. 2003.
Klarzynski, O., Descamps, V., Plesse, B.,Yvin, J. C., Kloareg, B. and Fritig, B. Sulfated fucan oligosaccharides elicit defense responses in tobacco and local and systemic resistance against tobacco mosaic virus. Molecular Plant- Microbe Interactions, 16, 115–122. 2003.
Cluzet, S. C., Torregrosa, C., Jacquet, C., Lafitte, J., Fournier, L., merci, S., Salamagne, S., Briand,X., Esquerre-Tugaye, M. T. and Dumas, B. Gene expression profiling and protection of Medicago Truncatula against a fungal infection in response to an elicitor from green algae Ulva spp. Plant Cell and Environment. 27, 917–928. 2004.
Jaulneau, V., Lafitte, C., Jacquet, C., Fournier, S., Salamagne, S., riand, X., Esquerr´e-Tugay´e, M. T., Dumas, B. Ulvan, asulfated polysaccharide from green algae, activates plant immunity through the jasmonic acid signaling pathway. Journal of Biomedicine and Biotechnology, 1-11. 2010. (ID 525291).
Lahaye, M. and Robic, A. Structure and functional properties of ulvan, a polysaccharide from green seaweeds. Bio macromolecules. 8:1765–1774. 2007.
Robic, A., Gaillard, C., Sassi, J. F., Lerat, Y. and Lahaye, M. Ultrastructure of ulvan: a polysaccharide from green seaweeds. Biopolymers, 91, 652–664. 2009a.
Robic, A., Sassi, J. F., Dion, P., Lerat, Y. and Lahaye, M. Seasonal variability of physicochemical and rheological properties of ulvan in two Ulva species (Chlorophyta) from the brittany coast. Journal of Phycology, 45, 962–970. 2009b.
Paulert, R., Ebbinghaus, D., Urlass, C. and Moerschbacher, B. M. Priming of the oxidative burst in rice and wheat cell cultures by ulvan, a polysaccharide from green macroalgae, and enhanced resistance against powdery mildew in wheat and barley plants. Plant Pathology, 59, 634–642. 2010.
Sbaihat, L., Takeyama, K., Koga, T., Takemoto, D., and Kawakita, K. Induced Resistance in Solanum lycopersicum by Algal Elicitor Extracted from Sargassum fusiforme. The Scientific World Journal. 2015.
Jaulneau,V ., Lafitte,C., Corio-Costet, M-F., Stadnik,M.J., Salamagne,S.J ., Briand ,X., Esquerré-Tugayé M.T.and Dumas, B. An Ulva armoricana extract protects plants against three powdery mildew pathogens. Eur J Plant Pathol ,131:393–401. 2011.
Moenne, A. Composition and Method to Stimulate Growth and Defense against Pathogens in Plants. 12,666,700. US Patent. 2009.
Araujo, L. and Stadnik, M.J. Cultivar-specific and ulvan-induced resistance of apple plants to Glomerella leaf spot are associated with enhanced activity of peroxidases. Acta Scientiarum. Agronomy. Maringá, V. 35, n. 3, p. 287-293. 2013.
El Modafar,C., Elgadda,M., El Boutachfaiti,R., Abouraicha,E., Zehhar,N., Petit,E., El Alaoui-Talibi,Z., Courtois,B and Courtois,J. Induction of natural defense accompanied by salicylic acid-dependant systemic acquired resistance in tomato seedlings in response to bioelicitors isolated from green algae. Scientia Horticulturae, Vol 138, p 55-63. 2012.
Peres, J. C. F., De Carvalho, L.R., Gonçalez1, L., Berian, L.O.S. and Joana D´arc Felicio,J. Evaluation of antifungal activity of seaweed. Ciênc. agrotec., Lavras, V 36, n. 3, p. 294-299. 2012.
Jayaraman, J., Jeff, N. and Zamir, P. Commercial extract from the brown seaweed Ascophyllum nodosum reduces fungal diseases in greenhouse cucumber. Journal of Applied Phycology, 23: 353-361. 2011.
Vera, J., Castro,J., Gonzalez, A. and Moenne, A. Seaweed Polysaccharides and Derived Oligosaccharides Stimulate Defense Responses and Protection Against Pathogens in Plants. Mar Drugs. (12):2514-25. 2011.
Jayaraj, J., Wan, A., Rahman, M. and Punja, Z.K., Seaweed extract reduces foliar fungal diseases on carrot. Crop Protection, Vol 27,10, 1360-1366. 2008.
De-Ascensao, A.F.R.D.C. and Dubrey, I. A. Soluble and wall-bound phenolic polymers in Musa acuminataroots exposed to elicitors from Fusariumoxysporumf.sp. cubens. Phytochemistry, 63, 679-686. 2003.
El- Khallal, S. M. Induction and Modulation of Resistance in Tomato Plants Against Fusarium Wilt Disease by Bioagent Fungi (Arbuscular Mycorrhiza) And/or Hormonal Elicitors (Jasmonic Acid& Salicylic Acid): 1- Changes in Growth, Some Metabolic Activities and Endogenous Hormones Related to Defence Mechanism. Australian Journal of Basic and Applied Sciences, 1(4): 691-705. 2007.
She-ze Z., Fan, Z. and Bao-zhenl, H. S. Enhancement of Phenylalanine Ammonia Lyase, Polyphenoloxidase, and Peroxidase in Cucumber Seedlings by Bemisiatabaci (Gennadius) (Hemiptera: Aleyrodidae) Infestation. Agricultural Sciences in China, 7(1), 82-87. 2008
Chérif, M., Arfaoui, A. and Rhaiem, A. Phenolic compounds and their role in bio-control and resistance of chickpea to fungal pathogenic attacks. Tunisian Journal of Plant Protection, 2: 7-21. 2007.
Thipyapong, P., Hunt, M. D. and Steffens, J. C. Antisense ownregulation of polyphenol oxidase results in enhanced disease susceptibility. Planta, 220, 105-117. 2004.
Mohammadi, M. and Karr, A. â -1,3-glucanase and chitinase activities in soybean root nodules. J. Plant Physiol., 159: 245-256. 2002.
Sin darovska, Y. R., Guzyk, O. I., Yuzvenko, L. V., Demchenko, O. A., Didenko, L. F., Grynevy, O. I. and Ya.Spivak. Ribonuclease activity of buck wheat plant (Fagopyrumesculentum) cultivars with different sensitivities to buckwheat burn virus. Ukr.Biochem. J. Vol. 86, N 3. 2014.
Sangaev, S. S., Kochetov, A. V., Ibragimova, S. S. Physiological role of extracellular ribonucleases of higher plants. Russ. J. Genetics: Appl. Res. 1, N 1. – P. 44–50. 2011.
Jaag, H. M. and Nagy, P. D. Host transcription factor Rpb11p affects tombusvirus replication and recombination via regulating the accumulation of viral replication proteins. Virology. 368(2):388-404. 2007.
Abou-ElWafa G. M. M. Sc. Thesis, Faculty of Science, Mansoura University, Mansoura (Egypt). 2005.
Flora, G. and Rani, S. M. V. An approach towards control of blast by foliar application of seaweed concentrate. Science Research Reporter 2(3): 213-217. 2012.
Zambonelli, A., Zechini D`Aulerio, A., Bianchi, A. and Albasini, A. Effects of essential oils on phytopathogenic fungi in vitro. J. Phytopathology 144, 491-494. 1996.
Harlapur, S. I., Kulkarni, M. S., Wali, M. C., and Srikantkulkarni, H. Evaluation of plant extracts, bio-agents and fungicides against Exserohilum turcicum causing Turcicum leaf blight of Maize. Journal of Agricultural Science, 20(3):541-544. 2007.
Finney D. J. Probit analysis, 3rd Edn. Cambridge University Press, Cambridge, UK, pp 1–333. 1971.
Cohen, Y., Gisi, U. and Mosinger, E. Systemic resistance of potato plants against Phytophthora infestans induced by unsaturated fatty acids. Physiol. Mol. Pl. Pathol., 38: 255-263. 1991.
Cohen, Y. Local and systemic control of Phytophthora infestans in tomato plants by DL 3 amino-n- butanoic acids. Phytopathology. 84, 55–59. 1994.
Slinkard, K. and Singleton, V.L. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and methods. American Journal of Enology and Viticulture, 28: 49-55. 1997.
Bradford, M. M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry 72, 248-254. 1976.
Dixon, R.A. Plant cell culture, a practical approach IRLPRESS. Oxford. Washington DC.1-235. 1985.
Broesch, S. Cholormetric assay of phenol oxidase.Bull.Sac.Chem.Biol.36:711-713. 1954.
Maranville, J.W. and Paulsen, M.P. Alternation of protein composition of corn (Zea Mays L.) seedling during moisture stress. Crop science, 120: 660-663. 1972.
Anonymous. SPSS Software Inc. Version 14.0 Statistical Package for Social Science. Chicago. IL, USA. 2005.
Abdel-Kader, M. M. and El-Mougy, N. S. (2013). Bioagents and Commercial Algae Products as Integrated Biocide Treatments for Controlling Root Rot Diseases of Some Vegetables under Protected Cultivation System. Journal of Marine Biology, 1-10.
Ambroziak, C., B. , Głosek-Sobieraj, M. and Kowalska,E. The effect of plant growth regulators on the incidence and severity of potato diseases. Pol. J. Natur. Sc., 30(1): 5–20. 2015.
Hammerschmidt, R. Phenols and plant-pathogen interactions: The saga continues. Physiol Mol Plant Pathol., 66: 77-78. 2005.
Abo-Elyousr, K.A.M, Hashem, M. and Ali, E.H. Integrated control of cotton root rot disease by mixing fungal biocontrol agents and resistance inducers. Crop Protection, 28, 295 – 301. 2009.
Ragab, M. M., Saber, M. M., El-Morsy, S. A. and Abd El-Aziz, A. R. M. Induction of Systemic Resistance Against Root Rot of Basil Using Some Chemical Inducers. Egypt. J. Phytopathol., 37, 1: 59-70. 2009.
Vimala, R. and Suriachandraselvan, M. Induced resistance in bhendi against powdery mildew by foliar application of salicylic acid. Journal of Biopesticides, 2(1): 111-114. 2009.
Garcia-Mina, J.M, Goicoechea, N. and Aguirreolea, J. Alleviation of Verticillium wilt in pepper (Capsicum annuum L) by using the organic amendment COA H ofnatural origin. Sci. Hortic. 101, 23–37. 2004
Ashok Kumar, N., Vanlalzarzova, B., Sridhar, S. and Baluswami, M., Effect of liquid seaweed fertilizer of Sargassum wightii grev. on the growth and biochemical content of green gram (Vigna radiata (L.) R. wilczek). Recent Research in Science and Technology, 4(4): 40-45. 2012.
Schroder, M., Hahlbrock, K. and Kombrink, E. Temporal and spatial patterns of β-1, 3-glucanase and chitinase induction in potato leave Infected by Phytophthora infestans. Plant J., 2: 161–172. 1992.
Wang, X., El Hadrami, A., Adam, L.R. and Daayf, F. Local and distal gene expression of pr-1 and pr-5 in potato leaves inoculated with isolates from the old (US-1) and the new (US-8) genotypes of Phytophthora infestans (Mont.) de Bary. Environ. Exp. Bot., 1–11. 2005.
Veronese, P., Crino, P., Tucci, M., Colucci, F., Yun, D.J., Hasegawa, M.P., Bressan, R.A. and Saccardo, F. Pathogenesis-related proteins for the control of fungal diseases of tomato. Genetics and Breeding for Crop Quality and Resistance Proceedings of the XV-EUCARPIA Congress, pp: 15–24. Viterbo, Italy, September, 20-25. 1999.
Melo, G. A., Shimizu, M. M. and Mazzafera, P. Polyphenoloxidase activity in coffee leaves and its role in resistance against the coffee leaf miner and coffee leaf rust. Phytochemistry. 67, 277–285. 2006.
Hassan, M. E. M., Abd El-Rahman, S. S., El-Abbasi, I. H. and Mikhail, M.S. Changes in peroxidase activity due to induced resistance against faba bean chocolate spot disease. Egypt. J. Phytopathol., 35: 35-48. 2007.
Thipyapong, P., Steffens, J.C. Tomato polyphenol oxidase (PPO): differential response of the PPO F promoter to injuries and wound signals. Plant Physiol 115:409–418. 1997.
Chirkov,S. N. , Il’ina, A. V. N., Surgucheva, A., Letunova, E. V. , Varitsev, Y. A., Tatarinova, N. Y. and Varlamov, V. P. Effect of Chitosan on Systemic Viral Infection and Some Defense Responses in Potato Plants, russian journal of plant physiology, Vol. 48, No. 6. 2001.
Downloads
Published
How to Cite
Issue
Section
License
- Papers must be submitted on the understanding that they have not been published elsewhere (except in the form of an abstract or as part of a published lecture, review, or thesis) and are not currently under consideration by another journal published by any other publisher.
- It is also the authors responsibility to ensure that the articles emanating from a particular source are submitted with the necessary approval.
- The authors warrant that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required.
- The authors ensure that all the references carefully and they are accurate in the text as well as in the list of references (and vice versa).
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.